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Effect of vasoactive intestinal polypeptide (VIP) on glucose and lipid metabolism of isolated rat adipocytes

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Research in Experimental Medicine

Summary

The biologic actions of vasoactive intestinal polypeptide (VIP) on insulin binding, glucose uptake and utilization, and on lipolysis were studied. At concentrations between 10−10 and 10−7 mol/l VIP influenced neither glucose uptake nor glucose incorporation into lipids under basal and insulin-stimulated conditions. This effect was independent of the presence of adenosine deaminase in the incubation medium. At 10−8 mol/l VIP increased insulin binding affinity slightly but not significantly, shifting the ID-50 from 12.4 ng/ml to 10.3 ng/ml, without any change in receptor number. However, VIP showed a marked dose-dependent lipolytic activity with the lowest effective concentration at 10−9 mol/l. At 10−6 mol/l glycerol release increased 7.3-fold as compared to basal lipolysis. In conclusion, VIP did not affect adipose tissue metabolism at physiologic concentrations. In the rare Verner-Morrison syndrome, however, the potent lipolytic activity of VIP may contribute to the metabolic disturbances observed.

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Authors and Affiliations

  1. Bonn—Bad-Godesberg, Federal Republic of Germany

    D. Kaminska (Fellow of the Alexander-von-Humboldt Stiftung)

  2. Abteilung Innere Medizin I, Universitätsklinik Ulm, Steinhövelstr. 9, D-7900, Ulm, Federal Republic of Germany

    H. Hauner, G. Glatting, D. Kaminska (Fellow of the Alexander-von-Humboldt Stiftung) & E. F. Pfeiffer

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  1. H. Hauner
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  2. G. Glatting
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  3. D. Kaminska
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  4. E. F. Pfeiffer
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Hauner, H., Glatting, G., Kaminska, D. et al. Effect of vasoactive intestinal polypeptide (VIP) on glucose and lipid metabolism of isolated rat adipocytes. Res. Exp. Med. 188, 189–195 (1988). https://doi.org/10.1007/BF01852320

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  • DOI: https://doi.org/10.1007/BF01852320

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