Summary
Thirty-six young rats were used to determine the effect of the fluoride on collagen synthesis in healing of fracture.
Eighteen rats received 100 ppm fluoride per day, the other 18 were not given fluoride and were used as controls. Then the tibiae of the 36 rats were successively fractured and the animals killed. That is, the first 14 animals of either group were killed one a day, and then the reamining four animals were killed on days 21, 28, 35, and 45 as the tibiae were fractured.
Collagen synthesis of the callus was examined histochemically and histologically. In the fluoride-treated group, collagen synthesis was found to be defective, while it was normal in the controls.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergman I, Loxley R (1963) Two improved and simplified methods for spectrophotometric determination of hydroxyproline. Anal Chem 35:1961
Bolognani W, Fawcett DW (1976) A textbook of histology, 8th ed. Saunders, Philadelphia, pp 176–178
Eastoe JE (1971) The organic matrix of bone. In: Bourne GH (ed) Biochemistry and physiology of bone. Academic Press, New York, p 81
Firsheim HE, Uristh MR (1972) Enzyme induction, accumulation of collagen and calcification in implants of bone matrix. Clin Orthop 84:263–275
Golub L, Glimcher MJ, Goldhaber P (1968) The effect of sodium fluoride on the rates of synthesis and degradation of bone collagen in tissue culture. Proc Soc Exp Biol Med 129:973–977
Hugh CB (1975) Resumé of fracture healing. Vet Clin North Am (Small Anim Pract) 5:147–156
Indian Council of Medical Research (1967) Microestimation of fluoride. Special report series, no 50. New Delhi
Ketenjian AY, Arsenis C (1975) Morphological and biochemical studies during differentiation and calcification of fracture callus. Clin Orthop 107:266–273
Nichols G Jr, Flanagan B (1966) Osteoporosis — A disorder of bone cell metabolism. Fed Proc 25:922–927
Peck WA, Zipkin I, Whedon GD (1970) Physiological effects of small doses of fluoride. In: WHO Monograph Series, no 59. Fluoride and human health. WHO, Geneva, p 187
Pentinen R (1972) Biochemical studies on fracture healing in the rat. Acta Chir Scand [Suppl] 432:6–32
Reddy GS, Srikantia SG (1971) Effect of dietary calcium, vit. C and protein in development of experimental skeletal fluorosis. Metabolism 20:642–656
Repo RU, Mitchell N (1971) Collagen synthesis in mature articular cartilage of the rabbit. J Bone Jt Surg (Br) 53-B:541–548
Sakamoto M, Sakamoto S, Parsons DB, Glimcher JM (1979) Collagen synthesis in embrionic chick-bone explants. J Bone Jt Surg (Am) 61-A:1042–1052
Steider A, Gedalia I, Schwarz A, Rodan A (1967) Effect of vit. D2 and fluoride on experimental bone fracture healing in rats. J Dental Res 46:675–686
Tietz NW (1970) Fundamentals of clinical chemistry. Saunders, Philadelphia London Toronto, p 188
Udupa KN, Prasad GC (1963) Chemical and histochemical studies on the organic constitutients in fracture repair in rats. J Bone Jt Surg (Br) 45-B:770–779
Uslu B, Bor N, Bozcuk N (1983) Effect of fluoride on fracture healing in the rats. Autoradiographical and histological studies. Hacet Bull Med Surg
Author information
Authors and Affiliations
Additional information
This study was carried out at Hacettepe University, Medical and Surgical Research Institute, Turkey
Rights and permissions
About this article
Cite this article
Uslu, B. Effect of fluoride on collagen synthesis in the rat. Res. Exp. Med. 182, 7–12 (1983). https://doi.org/10.1007/BF01852281
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01852281