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Breakdown of nonmitochondrial aspartate transaminase from pig heart in piglets

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Research in Experimental Medicine

Summary

Distribution of the radioactivity of labeled aspartate transaminase from the soluble fraction of pig heart was studied 8 hrs after intravenous injection in piglets in the tissue and the subcellular fractions.

The radioactivity was high in the total mass of muscle, followed by that in skin and the intestinal organs. Related to the weight of tissue, the radioactivity is high in kidney, lymph nodes, stomach, liver, and small intestine. It is low in fat, muscle and brain. On separation of the subcellular fractions by differential centrifugation it has been found that 60 to 80% of the PBI radioactivity occurs in the 100 000 g supernatant fraction in liver, renal cortex, and small intestine. Among the non-soluble fractions, the PBI radioactivity was significantly higher only in the mitochondrial fraction of the liver. 91% of the protein bound radioactivity in the sera can be precipitated with specific antibody at the time of the exsanguination. The precipitability of protein bound radioactivity with antibody in the 100 000 g supernatant fraction of renal cortex, liver, and small intestine amounted to 84, 81 and 91% resp. almost the same as in the sera.

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  1. Medizinische Poliklinik, Philipps-Universität, D-3550, Marburg, Federal Republic of Germany

    S. Massarrat

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  1. S. Massarrat
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Massarrat, S. Breakdown of nonmitochondrial aspartate transaminase from pig heart in piglets. Res. Exp. Med. 162, 257–266 (1974). https://doi.org/10.1007/BF01851698

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  • DOI: https://doi.org/10.1007/BF01851698

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