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Untersuchungen zur Funktion und Morphologie der Dünndarmschleimhaut der Ratte unter chronischem Glukagoneinfluß

Functional and morphological studies on intestinal mucosa of the rat under chronic glucagon application

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Research in Experimental Medicine

Summary

Chronic application (20 days) of glucagon in pharmacological doses induces mucosal transformation of the hyperregenerative type in the small intestine of the rat. This transformation is characterized by decreased villi, and increased crypt length.

The morphological changes are accompanied by a reduction in glucose absorption in vivo as well as by decreased activities of lactase, sucrase and maltase. The findings demonstrate that hyperglucagonemia is not the cause for hyperplastic mucosal transformation, which is found in the experimental diabetes in the rat.

Zusammenfassung

Chronische Zufuhr von Zinkprotamin-Glukagon in pharmakologischen Dosen bewirkt am Rattendünndarm nach 20 Tagen einen hyperregeneratorischen Schleimhautumbau. Dieser ist gekennzeichnet durch eine Verkleinerung der Zotten sowie der Gesamtzottenoberfläche und durch eine Zunahme der Kryptentiefen. Die Veränderungen werden begleitet von einer Verminderung der in vivo-Resorption der Glukose wie auch der Aktivitäten der Laktase, Saccharase und Maltase.

Die Befunde lassen den Schluß zu, daß die beim experimentellen Diabetes mellitus der Ratte beobachtete Schleimhauthyperplasie des Dünndarms nicht auf eine Hyperglukagonämie zurückzuführen ist.

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Literatur

  1. ASAKURA, H., TSUCHYA, M., WATANABE, J., ENOMITO, J., MORITA, A., MORISHITA, T., FUKUMI, H., OHASHI, M., CASTRO, A., UYLANGO, C.: Electron microscopic study on the jejunal mucosa in human cholera. Gut15, 531–544 (1974)

    PubMed  Google Scholar 

  2. AULSEBROOK, K.A.: Intestinal absorption of glucose and sodium: effect of epinephrine und norepinephrine. Biochem. Biophys. Res. Com.18, 165–169 (1965)

    PubMed  Google Scholar 

  3. BARBEZAT, G.O., GROSSMAN, M.T.: Intestinal secretion by peptides. Science174, 422–424 (1971)

    PubMed  Google Scholar 

  4. BLOCH, R., MENGE, H., LORENZ-MEYER, H., RIECKEN, E.O.: Morphological and biochemical changes of jejunal mucosa in rats with self-filling blind loops without and with bile diversion. In: Intestinal Adaptation. Ed. by H. Dowling, E.O. Riecken. p. 87–91 Stuttgart-New York: Schattauer 1974

    Google Scholar 

  5. BLOOM, S.R.: An enteroglucagon tumour. Gut.13, 520–523 (1972)

    PubMed  Google Scholar 

  6. CASPARY, W., LÜCKE, H.: Effect of chronic glucagon administration on the digestive and absorptive function of rat small intestine in vivo. Res. exp. Med.167, 1–13 (1976)

    Google Scholar 

  7. CLARKE, R.M.: Mucosal architecture and epithelial cell production rate in the small intestine of albino rat. J. Anatom.107, 519–529 (1970)

    Google Scholar 

  8. CRANE, R.K.: An effect of alloxan diabetes on the active transport of sugars by rat small intestine in vitro. Biochem. Biophys. Res. Com.4, 436–440 (1961)

    PubMed  Google Scholar 

  9. CROCKFORD, P.M., HAZZARD, W.R., WILLIAMS, R.H.: Insulin response to glucagon: the opposing effects of diabetes and obesity. Diabetes18, 216–224 (1969)

    PubMed  Google Scholar 

  10. DAHLQVIST, A.: A method for assay intestinal disaccharidases. Analyt. Biochem.7, 18–25 (1964)

    Google Scholar 

  11. DEBNAM, E.S., LEVIN, R.J.: Effects of fasting and semistarvation on the kinetics of active and passive sugar absorption across the small intestine in vivo. J. Physiol.252, 681–700 (1975)

    PubMed  Google Scholar 

  12. DIETSCHY, J.M., WESTERGAARD, H.: The effect of unstirred water layers on various transport processes in the intestine. In: Intestinal absorption and malabsorption. Ed. by T.Z. Csaky. p. 197–206 New York: Raven press 1975

    Google Scholar 

  13. DOMBROWSKI, H., PEMSEL, H.K.: Angiographie bei entzündlichen Dünndarmerkrankungen und beim Meserterialinfarkt. Radiologie14, 415–424 (1974)

    Google Scholar 

  14. FIELD, M.: Intestinal Secretion: Effect of cyclic AMP and its role in cholera. New Engl. J. Med.284, 1137–1144 (1971)

    PubMed  Google Scholar 

  15. GANGAROSA, E.J., BEISEL, W.R., BENYAJATI, C., SPRINZ, H., PIYARATN, P.: The nature of the gastrointestinal lesions in asiatic cholera and its relation to pathogenesis. Am. J. Topic. Med.9, 125–135 (1960)

    Google Scholar 

  16. GLEESON, M.H., BLOOM, S.R., POLAK, J.M., HENRY, K., DOWLING, R.H.: Endocrine tumour in kidney affecting small bowel structure motility and absorptive function. Gut.12, 773–782 (1971)

    PubMed  Google Scholar 

  17. GOTTESBÜREN, H., SCHMITT, E., MENGE, H., BLOCH, R., LORENZ-MEYER, H., RIECKEN, E.O.: Untersuchungen zum Einfluß des Insulins auf die intestinale Resorption beim Menschen. Res. exp. Med.161, 262–271 (1973)

    Google Scholar 

  18. GOTTESBÜREN, H., LEISING, H., MENGE, H., LORENZ-MEYER, H., RIECKEN, E.O.: Einfluß von Glukagon auf die Glukose-, Wasser- und Elektrolytresorption des menschlichen Jejunums. Klin. Wschr.52, 926–929 (1974)

    PubMed  Google Scholar 

  19. HENDRIX, T.R.: Cholera toxin and intestinal transport. In: Intestinal absorption and malabsorption. Ed. by T.Z. Csaky. p. 253–271 New York: Raven press 1975

    Google Scholar 

  20. JERVIS, E.L., LEVIN, R.J.: Anatomic adaptation of the alimentary tract of the rat hyperplasia of chronic alloxan diabetes. Nature, London210, 391–393 (1966)

    Google Scholar 

  21. KETTERER, H., EISENTRAUT, A.M., UNGER, R.H.: Effect upon insulin secretion of physiologic doses of glucagon administered via the portal vein. Diabetes16, 283–288 (1967)

    PubMed  Google Scholar 

  22. LORENZ-MEYER, H., KÖHN, R., RIECKEN, E.O.: Vergleich verschiedener morphometrischer Methoden zur Erfassung der Schleimhautoberfläche des Rattendünndarms und deren Beziehung zur Funktion. Histochemistry49, 123–129 (1976)

    PubMed  Google Scholar 

  23. LORENZ-MEYER, H., THIEL, F., MENGE, H., GOTTESBÜREN, H., RIECKEN, E.O.: Structural and functional studies on the transformation of the intestinal mucosa in rats with experimental diabetes. Res. exp. Med. (im Druck)

  24. LOWRY, O.H., ROSEBROUGH, N.J., FARR, A.L.: Protein measurement with folin phenol reagenz. J. Biol. Chem.193, 265–275 (1951)

    PubMed  Google Scholar 

  25. MENGE, H., BLOCH, R., SCHAUMLÖFFEL, E., RIECKEN, E.O.: Transportstudien, morphologische, morphometrische und histochemische Untersuchungen zum Verhalten der Dünndarmschleimhaut im operativ ausgeschalteten Jejunalabschnitt der Ratte. Z. ges. exp. Med.153, 74–90 (1970)

    Google Scholar 

  26. MILLER, D.L., SCHEDL, H.P.: Nonabsorbed indicators. A comparison of phenolred and insulin C14 and effect of perfusion technique. Gastroenterology62, 48–55 (1972)

    PubMed  Google Scholar 

  27. MÜLLER, W.A., FALOONA, G.R., UNGER, R.H.: The effect of experimental insulin deficiency on glucagon secretion. J. Clin. Invest.50, 1992–1999 (1971)

    PubMed  Google Scholar 

  28. MOORE, E.W., TAVANO, P.J.: The effects of peptide hormones on simultaneous jejunal and ileal water and electrolyte transport in the dog, II. Glucagon: some paradoxical effects. Gastroenterology66, 749 (1974)

    Google Scholar 

  29. NAGLER, R., FORREST, W., SPIRO, H.M.: Effect of glucagon on transfer of glucose utilizing everted sacs of small intestine. Am. J. Physiol.198, 1323–1325 (1960)

    PubMed  Google Scholar 

  30. NECHELEL, H., SPORN, J., WALKER, L.: Effect of glucagon on gastrointestinal motility. Am. J. Gastroenterology45, 34–40 (1966)

    Google Scholar 

  31. OLSEN, W., ROGERS, L.: Jejunal sucrase activity in diabetic rats. J. Lab. Clin. Med.77, 838–842 (1971)

    PubMed  Google Scholar 

  32. PEARSE, A.G.E.: Histochemistry. Theoretical and applied. 2nd ed. London: Churchill 1960

    Google Scholar 

  33. RIECKEN, E.O.: Die normale Dünndarmschleimhaut und ihre Veränderungen bei der einheimischen Sprue. Dt. Med. Wschr.95, 2295–2303 (1970)

    Google Scholar 

  34. RUDO, N.D.: Endogenous hyperglucagonemia as a mediator of intestinal transport in starvation: Studies with antiglucagon antibody. Gastroenterology64, 686 (1973)

    Google Scholar 

  35. RUDO, N.D., ROSENBERG, J.H.: Chronic glucagon administration enhances intestinal transport in rats. Proc. Exp. Biol.142, 521–525 (1973)

    Google Scholar 

  36. SCHEDL, H.P., WILSON, H.D.: Effects of diabetes on intestinal growth and hexose transport in the rat. Am. J. Physiol.220, 1739–1745 (1971)

    PubMed  Google Scholar 

  37. SCHMIDT, F.H.: Die enzymatische Bestimmung der Glukose und Fruktose nebeneinander. Klin. Wschr.39, 1244–1247 (1961)

    PubMed  Google Scholar 

  38. UNGER, R.H.: Glucagon physiology and pathophysiology. New Engl. J. Med.285, 443–449 (1971)

    PubMed  Google Scholar 

  39. VARRO, V., CSERNAY, L.: The effect of intraarterial insulin and glucagon on the glucose metabolism of the small intestine in the dog. Scand. J. Gastroenterology1, 232–237 (1966)

    Google Scholar 

  40. Wissenschaftliche Tabellen. Documenta Geigy, Geigy AG, Pharma Basel 1968

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Authors and Affiliations

  1. Medizinische Universitätsklinik, Mannkopffstraße 1, D-3550, Marburg/Lahn, Germany

    H. Lorenz-Meyer, H. Menge & E. O. Riecken

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  1. H. Lorenz-Meyer
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  2. H. Menge
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  3. E. O. Riecken
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Mit Unterstützung der Deutschen Forschungsgemeinschaft (Ri 139/10)

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Lorenz-Meyer, H., Menge, H. & Riecken, E.O. Untersuchungen zur Funktion und Morphologie der Dünndarmschleimhaut der Ratte unter chronischem Glukagoneinfluß. Res. Exp. Med. 170, 181–192 (1977). https://doi.org/10.1007/BF01851389

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  • DOI: https://doi.org/10.1007/BF01851389

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