Abstract
The characteristics of a strong mouse alloantigen with renal, bone marrow, and lymphoid expression were studied. This antigen is probably identical to that currently designated Ly-6.2. It was defined by the high-titered (1:1000) cytotoxic activity of three different antisera against peripheral lymphocyte target cells from DBA/2, DBA/1, and a variety of other strains. In the F2 and four backcross generations the genetic control of this specificity segregated as a single autosomal dominant gene. In lymphoid tissues the predominant expression was on T cells but 10–30% of B cells were lysed by these antisera. The specificity was expressed strongly in kidney, as shown by sequential absorption, in amounts equal to or greater than the amount in lymphoid tissues. Comparison to the rate of absorption of H-2 by kidney indicated that this antigen may be expressed in amounts comparable to an H-2 antigen in kidney. Immunization with kidney tissue resulted in a strong cytotoxic antibody response. The number of bone marrow cells expressing this antigen (40–50%) was well beyond what could be accounted for by T lymphocytes in bone marrow. In addition, a nonlymphoid tumor, the P815Y mastocytoma, was positive by cytotoxicity and by absorption. The extensive nonlymphoid expression and antigenic strength of Ly-6.2 raises the possibility that this serologically defined lymphocyte alloantigen will have histocompatibility effects when allografts of the appropriate tissues are examined.
Article PDF
Similar content being viewed by others
References
Amos, D.B., Bashir, H., Boyle, W., MacQueen, M., and Tiilikainen, A.: A simple micro cytotoxicity test.Transplantation 7:220–223, 1969
Amos, D.B., Haughton, G., and Spencer, R.A.: A serological analysis and comparison of various preparations of mouse H-2 histocompatibility antigen.Immunology 5:370–381, 1963
Boyse, E.A., Hubbard, L., Stockert, E., and Lamm, M.E.: Improved complementation in the cytotoxic test.Transplantation 10:446–449, 1970
Boyse, E.A., Cantor, H., Shen, F.-W., McKenzie, I.F.C.: Nomenclature for antigens demonstrable on lymphocytes.Immunogenetics 5:189, 1977.
Cantor, H. and Boyse, E.A.: Lymphocytes as models for the study of mammalian cellular differentiation.Immunol. Rev. 33:105–123, 1977
Carpenter, C.B., D'Apice, A.J.F., and Abbas, A.K.: The role of antibodies in the rejection and enhancement of organ allografts.Adv. Immunol. 22:1–65, 1976
Davies, H.ff.S. and Butcher, G.W.: Kidney alloantigens determined by two regions of the rat major histocompatibility complex.Immunogenetics 6:171–181, 1978
Dunn, T.B. and Potter, M.: A transplantable mast cell neoplasm in the mouse.J. Natl. Cancer Inst. 18:587–595, 1957
Frelinger, J.A., Neiberhuber, J.E., David, C.S., and Shreffler, D.C.: Evidence for the expression of la (H-2 associated) antigens on thymus-derived lymphocytes.J. Exp. Med. 140:1273–1284, 1974
Frelinger, J.A. and Murphy, D.B.: A new alloantigen, Ly-8, recognized by C3H anti-AKR serum.Immunogenetics 3:481–487, 1976
Graziano, K.D. and Edidin, M.: Serological quantitation of histocompatibility-2 antigens and the determination of H-2 in adult and fetal organs.In A. Lengerova and M. Vojtíšková (eds.):Immunogenetics of the H-2system; pp. 251–256, Karger, Basel, 1971
McKenzie, I.F.C. and Snell, G.D.: Ly-4.2: A cell membrane alloantigen of murine B lymphocytes. I. Population studies.J. Immunol. 114:848–855, 1975
McKenzie, I.F.C., Cherry, M., and Snell, G.D.: Ly-6.2: A new lymphocyte specificity of peripheral T cells.Immunogenetics 5:25–32, 1977
Murphy, D.B. and Shreffler, D.C.: Cross-reactivity between H-2K and H-2D products. I. Evidence for extensive and reciprocal serological cross-reactivity.J. Exp. Med. 141:374–391, 1975
Paris, A.M.I., Bishop, C., Festenstein, H., and Günther, E.: Non-major histocompatibility system immunogenetic influences in rat renal allograft survivalTransplantation 25:252–254, 1978
Pizzaro, O., Hoecker, G., Rubinstein, P., and Ramos, A.: The distribution in the tissues and the development of H-2 antigens on the mouse.Proc. Natl. Acad. Sci. U.S.A. 47:1900–1907, 1961
Raff, M.C.: Surface antigenic markers for distinguishing T and B lymphocytes in mice.Transplant. Rev. 6:52–80, 1971
Russell, P.S., Chase, C.M., Colvin, R.B., and Plate, J.M.D.: Kidney transplants in mice. An analysis of the immune status of mice bearing long-term H-2 incompatibile transplants.J. Exp. Med. 147:1469–1486, 1978
Sachs, J.A., Huber, B., Pena-Martinez, J., and Festenstein, H.: Genetic studies and effect on skin allograft survival of DBA/2 DAG, Ly, and M-locus antigens.Transplant. Proc. 5:1385–1387, 1973
Sanderson, A.R.: Quantitative titration, kinetic behaviour, and inhibition of cytotoxic mouse isoantisera.Immunology 9:287–300, 1965
Snell, G.D., Dausset, J., and Nathenson, S.:Histocompatibility; pp. 67–90, Academic Press, New York, 1976
Snell, G.D.: Genetic symbols: How do we get good ones?Immunogenetics 5:389–392, 1977
Thoenes, G.H.: Induction of unresponsiveness to organ transplants in congenic strains of rats and other mammals.Adv. Exp. Med. Biol. 64:457–466, 1975
Thoenes, G.H., Urban, G., and Doering, I.: Kidney transplantation between congenic versus standard inbred strains of rats: I. The significance of H-1 and non-H-1 gene differences.Immunogenetics 1:239–253, 1974
Van Rood, J.J., Van Leeuwn, A., and Ploem, J.S.: Simultaneous detection of two cell populations by two-color fluorescence and application to the recognition of B-cell determinants.Nature 262:795–797, 1976
Wigzell, H.: Quantitative titrations of mouse H 2 antibodies using Cr51-labelled target cells.Transplantation 3:423–431, 1965
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Halloran, P.F., Dutton, D., Chance, H. et al. An Ly-like specificity with extensive nonlymphoid expression. Immunogenetics 7, 185–200 (1978). https://doi.org/10.1007/BF01844007
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01844007