Veterinary Research Communications

, Volume 19, Issue 3, pp 179–184 | Cite as

Training-induced modifications in some biochemical defences against free radicals in equine erythrocytes

  • L. Avellini
  • M. Silvestrelli
  • A. Gaiti


Oxidative stress develops when the generation of free radicals exceeds the antioxidant capacity of cells or extracellular fluids. It can also occur as a result of physical exercise, and the pathogenesis of exercise-induced myopathies and haemolysis in horses may be related to changes in lipid peroxidation caused by free radicals. Cells have developed biochemical protection against oxidative stress and, as tissues seem to increase their antioxidant defences under chronic activation, training may be one of the ways of increasing antioxidant defences. Accordingly, we tested some enzymatic antioxidant activities as well as nonenzymatic antioxidants in horses undergoing special training. The results indicated a decrease in both chemical and biochemical defences against free radicals during training. It was deduced that the horses' diet may have been unable to provide the increased need for antioxidant defences resulting from training.


α-toxopherol antioxidant erythrocytes free radicals glutathione peroxidase horse oxidative stress training vitamin E 





enzymic unit


glutathione peroxidase


degree of fluorescence polarization

Phospholipid P

phosphorous content in phospholipids


red blood cell




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  1. Avellini, L., Spaterna, A., Reboldi, P. and Gaiti, A., 1993. Defence mechanisms against free radical-induced damages in sheep, cattle and dog erythrocytes.Comparative Biochemistry and Physiology,106B, 391–394Google Scholar
  2. Barja De Quiroga, G., 1992. Brown fat thermogenesis and exercise: two examples of physiological oxidative stress?Free Radical Biology and Medicine,13, 325–340Google Scholar
  3. Brady, P.S., Ku, P.K. and Ullrey, D.E., 1978. Lack of effect of selenium supplementation on the response of the equine erythrocyte glutathione system and plasma enzymes to exercise.Journal of Animal Science,47, 492–496Google Scholar
  4. Chow, C.K., 1991. Vitamin E and oxidative stress.Free Radical Biology and Medicine,11, 215–232Google Scholar
  5. Combs, G.F. Jr, and Combs, G.F., 1984. The nutritional biochemistry of selenium.Annual Review of Nutrition,26, 257–280Google Scholar
  6. Deuticke, B., Heller, K.B. and Haest, C.W.M., 1986. Leak formation in human erythrocytes by the radical-forming oxidant t-butylhydroperoxide.Biochimica et Biophysica Acta,854, 169–183Google Scholar
  7. Duthie, G.G., Robertson, J.D., Maughan, R.J. and Morrice, P.C., 1990. Blood antioxidant status and erythrocyte lipid peroxidation following distance running.Archives of Biochemistry and Biophysics,282, 78–83Google Scholar
  8. Gatti, C., Noremberg, K., Brunetti, M., Teolato, S., Calderini, G. and Gaiti, A., 1986. Turnover of palmitic and arachidonic acids in the phospholipids from different brain areas of adult and aged rats.Neurochemical Research,11, 241–252Google Scholar
  9. Haenen, G.R.M.M., Tsoi, J.N.L.T.T., Vermeulen, N.P.E., Timmerman, H. and Bast, A., 1987. 4-Hydroxy-2,3-trans-nonenal stimulates microsomal lipid peroxidation by reducing the glutathione-dependent protection.Archives of Biochemistry and Biophysics,259, 449–456Google Scholar
  10. Kagan, V.E., 1988.Lipid peroxidation in biomembranes, (CRC Press, Boca Raton)Google Scholar
  11. Kagan, V.E., Bakalova, R.A., Serbinova, E.E. and Stoytchev, T.S., 1990. Fluorescence measurements of incorporation and hydrolysis of tocopherol and tocopheryl esters in biomembranes.Methods in Enzymology,186, 355–367Google Scholar
  12. Lentz, B.R., 1988. Membrane fluidity from fluorescent anisotropy measurements. In: L.M. Loew (ed.),Spectroscopic Membrane Probes (CRC Press, Boca Raton), 13–41Google Scholar
  13. Litman, B.J. and Barenholz, Y., 1982. Fluorescent probe: diphenylhexatriene.Methods in Enzymology,81, 678–685Google Scholar
  14. Machlin, L.J., 1980.Vitamin E, A Comprehensive Treatise, (Marcel Dekker, New York)Google Scholar
  15. Martin-Rosset, W., 1990.L'alimentation des chevaux. (INRA Press, Paris)Google Scholar
  16. Matsuki, N., Tamura, S., Ono, K., Watari, T., Goitsuka, R., Yamnobe, A., Hiraga, A., Kubo, K., Takagi, S. and Hasegawa, A., 1991. Exercise-induced phospholipid degradation in the equine skeletal muscle and erythrocytes.Journal of Veterinary Medical Sciences,53, 1001–1007Google Scholar
  17. Ono, K., Inui, K., Hasegawa, T., Matsuki, N., Watanabe, H., Takagi, S., Hasegawa, A. and Tomoda, I., 1990. The change of antioxidative enzyme activities in equine erythrocytes following exercise.Japanese Journal of Veterinary Sciences,52, 759–765Google Scholar
  18. Packer, L., 1984. Vitamin E, physical exercise and tissue damage in animals.Medical Biology,62, 105–109Google Scholar
  19. Pigeolet, E. and Remacle, J., 1991. Susceptibility of glutathione peroxidase to proteolysis after oxidative alteration by peroxides and hydroxyl radicals.Free Radical Biology and Medicine,11, 191–195Google Scholar
  20. Shanmugasundaram, K.R., Padmavathi, C., Acharya, S., Vidhyalakshmi, N. and Vijayan, V.K., 1992. Exercise-induced cholesterol depletion and Na+,K+-ATPase in human red cell membrane.Experimental Physiology,77, 933–936Google Scholar
  21. Shinitzky, M. and Barenholz; Y., 1978. Fluidity parameters of lipid regions determined by fluorescence polarization.Biochimica et Biophysica Acta,515, 367–394Google Scholar
  22. Wendel, A., 1981. Glutathione peroxidase.Methods in Enzymology,77, 325–333Google Scholar
  23. Winterbourn, C.C., 1990. Oxidative reactions of haemoglobin.Methods in Enzymology,186, 265–272Google Scholar
  24. Witt, E.H., Reznick, A.Z., Viguie, C.A., Stark Reed, P. and Paker, L., 1992. Exercise, oxidative damage and effect of antoxidant manipulation.Journal of Nutrition,122, 766–773Google Scholar

Copyright information

© Kluwer Academic Publishers bv 1995

Authors and Affiliations

  • L. Avellini
    • 1
  • M. Silvestrelli
    • 2
  • A. Gaiti
    • 1
  1. 1.Istituto di Biochimica e Chimica MedicaUniversità di PerugiaPerugiaItaly
  2. 2.Centro del Cavallo Sportivo, Facoltà di Medicina VeterinariaUniversità di PerugiaPerugiaItaly

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