Summary
The expression of estrogen (ER) and progesterone (PR) receptors was assayed by steroid binding in a series of 95 malignant breast tumors, for which the analysis of chromosome aberrations was performed and allowed the reconstruction of their chromosomal evolution. It was shown that breast tumors undergo a progressive loss of chromosomes, with occasionally one and rarely two endoreduplications. Chromosome losses were often the consequence of rearrangements, and the rate of rearranged chromosomes, which increases progressively, appeared as a possible indicator of tumor progression. The distribution of ER and PR values in the sample of 95 tumors was compared to that of a larger control series of consecutive cases: 598 for ER and 460 for PR. The similarities of the distributions indicated that the sample of 95 tumors was representative of the general population of breast cancers. The levels of ER and PR expression were very strongly and negatively correlated to the rate of rearranged chromosomes, but not to the modal number of chromosomes. However, when tumors having either undergone endoreduplication or not (>50 or <51 chromosomes, respectively) were considered separately, a significant correlation between ER and PR expression and chromosome number was found within each group. Finally, breast cancers were subdivided into 4 stages of cytogenetic evolution, from the least to the most evolved: stage 1: ≤50 chromosomes, <25% rearranged chromosomes; stage 2: >50 chromosomes, <25% rearranged chromosomes; stage 3: ≤50 chromosomes, >25% rearranged chromosomes; stage 4: >50 chromsomes, >25% rearranged chromosomes. The rate of negative or low ER values (<500 fmol/g tissue) was 10% in stage 1; 16% in stage 2; 45% in stage 3; 82% in stage 4. The corresponding rates for negative or low PR values (<500 fmol/g tissue) were 17%; 28%; 60%; and 91%. These data illuminate the probable role of genetic evolution in determining the variations of biological prognostic parameters of breast cancer, such as steroid hormone receptors.
Similar content being viewed by others
References
Dutrillaux B, Gerbault-Seureau M, Remvikos Y, Zafrani B, Prieur M: Breast cancer genetic evolution. I. Data from cytogenetics and DNA content. Breast Cancer Res Treat 19: 245–255, 1991
Benner SE, Clark GM, McGuire WL: Steroid receptors, cellular kinetics and lymph node status as prognostid factors in breast cancer. Am J Med 296: 59–66, 1988
Mason BH, Holdaway IM, Mullins PR, Yee LH, Kay RG: Progesterone and estrogen receptors as prognostic variables in breast cancer. Cancer Res 43: 2985–2990, 1983
Magdelenat H, Pouillart P, Jouve M, Palangié T, Garcia-Giralt E, Bretaudeau B, Asselain B: Progesterone receptor as a more reliable prognostic parameter than estrogen receptor in patients with advanced breast cancer. Breast Cancer Res Treat 2: 195–196, 1982
EORTC Breast Cooperative Group: Revision of the standards for the assessment of hormone receptors in human breast cancer. Eur J Cancer 16: 1513–1515, 1980
Magdelenat H: Forage biopsie et cytoponction pour la détermination des récepteurs hormonaux. Pathologie Biologie 31: 755–760, 1985
Goussard J, Lechevrel C, Martin PM, Roussel G: Détermination des récepteurs d'oestrogènes par anticorps monoclonaux dans 160 tumeurs mammaires: comparaison de la méthode immunoenzymatique avec la méthode au charbon dextran. Bull Cancer 72: 168–182, 1985
Gerbault-Seureau M, Vielh P, Zafrani B, Salmon R, Dutrillaux B: Cytogenetic study of twelve human near diploid breast cancers with chromosomal changes. Ann Génét 30: 138–145, 1987
Dutrillaux B, Couturier J: La pratique de l'analyse chromosomique. Ed Masson Paris, 1981
Dutrillaux B, Gerbault-Seureau M, Zafrani B: Characterization of chromosomal anomalies in human breast cancer. Cancer Genet Cytogenet 49: 203–217, 1990
McGuire WL, Tandon AK, Allred DC, Chamness GC, Clark GM: How to use prognostic factors in axillary node negative breast cancer patients. J Natl Cancer Inst 82: 1006–1015, 1990
Dutrillaux B, Gerbault-Seureau M, Zafrani B, Prieur M: Prolonged G2-phase of breast cancer cells and chromosome damage. Eur J Cancer, in press, 1991
Bichel S, Skovgaard-Poulsen H, Andersen J: Estrogen receptor content and ploidy of human mammary carcinoma. Cancer 50: 1771–1775, 1982
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Magdelenat, H., Gerbault-Seureau, M., Laine-Bidron, C. et al. Genetic evolution of breast cancer: II. relationship with estrogen and progesterone receptor expression. Breast Cancer Res Tr 22, 119–127 (1992). https://doi.org/10.1007/BF01833341
Issue Date:
DOI: https://doi.org/10.1007/BF01833341