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On the Y fluorescence in human male fibroblasts

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Summary

In a system of mitotically synchronized human fibroblast cells of the karyotype 46, XY and 47,XYY, the frequency and the variation in structure of the quinacrine dihydrochloride stained heterochromatic part of the Y chromosome was counted stepwise in regard to the periods of the cell cycle. There was no change of frequency and structure in correlation to these periods, especially no visible uncoiling in relation to the time of DNA synthesis.

Zusammenfassung

In einem System teilungssynchroner Kulturen menschlicher Fibroblasten des Karyotyp 46,XY und 47,XYY wurden Häufigkeiten und Strukturen des mit Quinacrindihydrochlorid gefärbten heterochromatischen Anteils des Y-Chromsoms in Abhängigkeit von den einzelnen Phasen des Zellcyclus fortlaufend beobachtet. Es fand sich keine Veränderung in Korrelation zu den Cyclusphasen, insbesondere keine meßbare Entspiralisation des heteropyknotischen F-body in Verbindung mit dem Zeitraum des DNS-Synthese.

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Authors and Affiliations

  1. Institut für Humangenetik der Philipps-Universität Marburg a. d. L., Marburg, Germany

    Heiner Cramer & Susanne Hansen

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  1. Heiner Cramer
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  2. Susanne Hansen
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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Cramer, H., Hansen, S. On the Y fluorescence in human male fibroblasts. Hum Genet 17, 23–28 (1972). https://doi.org/10.1007/BF01789596

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  • DOI: https://doi.org/10.1007/BF01789596

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