Summary
ARhizobium strain which exhibits high efficiency of starformation has been isolated from the root nodules ofLupinus luteus. Mutants of this strain were induced by repeated treatment of the wild type with nitrous acid. The mutants are marked with one or two auxotrophic characters and with different colors (carotenoid production). Two-, three-, and four-point crosses were performed. The maximum recombination rate is around 10%. A quantitative evaluation of the results of the crosses indicates the existence of a circular linkage map.
The recombination is the result of a conjugation between cells during which most probably always the whole chromosome is transferred.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Czygan, F. Ch., and W.Heumann: Composition and Biogenesis of the Carotenoids inPseudomonas echinoides and some mutants. Arch. Mikrobiol.57, 123–134 (1967).
Demerec, M., E. A.Adelberg, A. J.Clark, and P. E.Hartman: A porposal for a uniform nomenclature in bacterial genetics. Genetics54, 61–76 (1965).
Heumann, W.: Der Sexualzyklus sternbildender Bakterien. Arch. Mikrobiol.24, 362–395 (1956).
—: Kreuzungsversuche, mit sternbildenden Bakterien. Arch. Mikrobiol.36, 244–256 (1960a).
— Versuche zur Rekombination sternbildender Bakterien. Naturwissenschaften47, 330–331 (1960b).
— Die Methodik der Kreuzung sternbildender Bakterien. Biol. Zbl.81, 341–354 (1962a).
— Das genetische Verhalten sternbildender Bakterien. Naturwissenschaften49, 430–431 (1962c).
— Genetische Untersuchung sternbildender Bakterien. Z. Vererbungsl93, 441–452 (1962d).
—, u.Ruth Marx: Feinstruktur und Funktion der Fimbrien, bei dem sternbildenden Bakterium,Pseudomonas echinoides. Arch. Mikrobiol.47, 325–337 (1964).
Jacob, F., et S.Brenner: Sur la régulation de la synthèse du DNA chez les bactéries: l'hypothèse du réplicon. C. R. Acad. Sci. (Paris)256, 298–300 (1963).
——, et S.Brenner, and F.Cuzin: On the regulation of DNA replication in bacteria. Cold Spr. Harb. Symp. quant. Biol.28, 329–348 (1963).
—, and E. L.Wollman: Sexuality and the genetics of bacteria. New York: Academic Press, Inc. 1961.
Kleinkauf, H.: Carotine und Carotinoid-Synthese inRhizobium lupini-Mutanten. Arch. Mikrobiol.53, 154–158 (1966).
Knösel, D.: Prütung von Bakterien auf Fähigkeit zur Sternbildung. Zbl. Bakt., II. Abt.116, 70–100 (1962).
Lederberg, J.: Isolation and characterization of biochemical mutants of bacteria. Meth. med. Res.3, 5–22 (1950).
—, and Esther M.Lederberg: Replica plating and indirect selection of bacterial mutants. J. Bact.63, 399–406 (1952).
—, and E. L.Tatum: Novel genotypes in mixed cultures of biochemical mutants of bacteria. Cold Spr. Harb. Symp. quant. Biol.11, 113–114 (1946).
—, and N. D.Zinder: Concentration of biochemical mutants of bacteria with penicillin. J. Amer. chem. Soc.70, 4267 (1948).
Marx, Ruth, u. W.Heumann: Über Geisselfeinstrukturen und Fimbrien bei zweiPseudomonas-Stämmen. Arch. Mikrobiol.43, 245–254 (1962).
Author information
Authors and Affiliations
Additional information
Communicated byF. Kaudewitz
The research was supported by NSF Grant. No. GB-4287 and NJH Grant No. 1 PO 1 GM 13234.
Rights and permissions
About this article
Cite this article
Heumann, W. Conjugation in starformingRhizobium lupini . Molec. Gen. Genet. 102, 132–144 (1968). https://doi.org/10.1007/BF01789140
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01789140