Skip to main content
Log in

A specialised transducing phage of P22 for which the ability to form plaques is associated with transduction of theproAB region

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The multisite mutantproAB47 has been used to isolate specialised transducing phages of P22 for theproA andproB genes. Several of the preparations contain phages that form small plaques, and many of the properties of these suggest that the small plaques are formed by the transducing phages. Preliminary studies on the transduction of theproAB region by one such phage suggest that it forms fairly stable heterogenotes, both from apro point mutant and fromproAB47. The fact that the latter mutant is missing the phage attachment site results in a delay in integration of the specialised transducing phage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Adams, M. H.: Bacteriophages p. 456. New York: Interscience Publishers Inc. 1959.

    Google Scholar 

  • Benzinger, R.: Phage released fromS. typhimurium lysogenized by P22. Virology18, 643–645 (1962).

    PubMed  Google Scholar 

  • Brenner, S., Horne, R. W.: A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta (Amst.)34, 103–110 (1959).

    Google Scholar 

  • Dawson, G. W. P., Howe, T. G. B.: Transduction ofpro + toSalmonella typhimurium proAB47 by phage P22. J. gen. Microbiol.58, 65–67 (1969).

    PubMed  Google Scholar 

  • Hershey, A. D., Chase, M.: Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. gen. Physiol.36, 39–59 (1952).

    PubMed  Google Scholar 

  • Luria, S. E., Adams, J. N., Ting, R. C.: Transduction of lactose-utilising ability among strains ofE. coli andS. dysenterial and the properties, of the transducing phage particles. Virology12, 348–390 (1960).

    PubMed  Google Scholar 

  • Manly, K., Signer, E. R., Radding, C. M.: Nonessential functions of bacteriophage λ. Virology37, 177–188 (1969).

    PubMed  Google Scholar 

  • Matshushiro, A., Sato, K., Kida, S.: Characteristics of the transducing elements of bacteriophage Ø80. Virology23, 299–306 (1964).

    PubMed  Google Scholar 

  • Miyake, T., Demerec, M.: Proline mutants ofSalmonella typhimurium. Genetics45, 755–762 (1960).

    Google Scholar 

  • Patai Wing, J.: Transduction by P22 in a recombination-deficient mutant ofSalmonella typhimurium. Virology36, 271–276 (1968).

    PubMed  Google Scholar 

  • Rao, R. N., Smith, H. O.: Phage P22 lysogens of aSalmonella typhimurium mutant deleted at the normal prophage attachment site. Virology36, 328–330 (1968).

    PubMed  Google Scholar 

  • Smith, H. O.: Defective phage formation by lysogens of integration deficient phage P22 mutants. Virology34, 203–223 (1968).

    PubMed  Google Scholar 

  • —, Levine, M.: Gene order in prophage P22. Virology27, 229–231 (1965).

    PubMed  Google Scholar 

  • Smith-Keary, P. F.: Restricted transduction by bacteriophage P22 inSalmonella typhimurium. Genet. Res.8, 73–82 (1966).

    PubMed  Google Scholar 

  • Thomas, R.: Lysogeny. Symp. Soc. gen. Microbiol.18, 315–342 (1968).

    Google Scholar 

  • Young, B. G., Hartman, P. E.: Sites of P22 and P221 prophage integration inSalmonella typhimurium. Virology28, 265–270 (1966).

    PubMed  Google Scholar 

  • Yura, T.: Suppressor mutation in purine-requiring mutants ofSalmonella typhimurium. Genetic studies with bacteria. Publ. Carneg. Instn.612, 73–75 (1956).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by P. Starlinger

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jessop, A.P. A specialised transducing phage of P22 for which the ability to form plaques is associated with transduction of theproAB region. Molec. Gen. Genet. 114, 214–222 (1972). https://doi.org/10.1007/BF01788890

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01788890

Keywords

Navigation