Summary
The reconstitution process of an infectious tobacco mosaic virus particle from its RNA and protein consists of two steps, formation of the initial complex and growth of the helical rod, the former is the rate limiting step. The protein aggregate, having about 20–30 S, is needed for the formation of the initial complex with 5′-end of tobacco mosaic virus RNA. The elongation reaction from the initial complex proceeds even under conditions where both the reconstitution reaction and the formation of 20–30 S protein aggregates do not take place. This indicates that the growth of the helical rod proceeds by stepwise additions of protein subunits or 4 S aggregates. A possible model for assembly process of tobacco mosaic virus particle is presented.
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Communicated by H. Ozeki
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Okada, Y., Ohno, T. Assembly mechanism of tobacco mosaic virus particle from its ribonucleic acid and protein. Molec. Gen. Genet. 114, 205–213 (1972). https://doi.org/10.1007/BF01788889
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DOI: https://doi.org/10.1007/BF01788889