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Molecular and General Genetics MGG

, Volume 114, Issue 3, pp 205–213 | Cite as

Assembly mechanism of tobacco mosaic virus particle from its ribonucleic acid and protein

  • Yoshimi Okada
  • Takeshi Ohno
Article

Summary

The reconstitution process of an infectious tobacco mosaic virus particle from its RNA and protein consists of two steps, formation of the initial complex and growth of the helical rod, the former is the rate limiting step. The protein aggregate, having about 20–30 S, is needed for the formation of the initial complex with 5′-end of tobacco mosaic virus RNA. The elongation reaction from the initial complex proceeds even under conditions where both the reconstitution reaction and the formation of 20–30 S protein aggregates do not take place. This indicates that the growth of the helical rod proceeds by stepwise additions of protein subunits or 4 S aggregates. A possible model for assembly process of tobacco mosaic virus particle is presented.

Keywords

Mosaic Virus Virus Particle Assembly Process Tobacco Mosaic Virus Protein Aggregate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Butler, P. J. G., Klug, A.: Assembly of the particle of tobacco mosaic virus from RNA and disks of protein. Nature New Biology229, 47–50 (1971).PubMedGoogle Scholar
  2. Casper, D. L. D.: Assembly and stability of the tobacco mosaic virus particle. Advanc. Protein Chem.18, 37–121 (1963).Google Scholar
  3. Durham, A. C. H., Finch, J. T., Klug, A.: States of aggregation of tobacco mosaic virus protein. Nature New Biology.229, 37–42 (1971).PubMedGoogle Scholar
  4. —, Klug, A.: Polymerization of tobacco mosaic virus protein and its control. Nature New Biology229, 42–46 (1971).PubMedGoogle Scholar
  5. Fraenkel-Conrat, H.: The role of the nucleic acid in the reconstitution of active tobacco mosaic virus. J. Amer. chem. Soc.78, 882–883, (1956).Google Scholar
  6. —: Degradation of tobacco virus with acetic acid. Virology4, 1–4 (1957).PubMedGoogle Scholar
  7. —, Singer, B.: Reconstitution of tobacco mosaic virus. III. Improved methods and the use of mixed nucleic acid. Biochim. biophys. Acta (Amst.)33, 359–370 (1959).Google Scholar
  8. ——: Reconstitution of tobacco mosaic virus. IV. Inhibition by enzymes and other protein and use of polynucleotide. Virology23, 354 (1964).PubMedGoogle Scholar
  9. ——, Tsugita, A.: Purification of viral RNA by means of bentonite. Virology14, 54–58 (1961).PubMedGoogle Scholar
  10. —, Williams, R. C.: Reconstitution of active tobacco mosaic virus from its inactive protein and nucleic acid components. Proc. nat. Acad. Sci. (Wash.)41, 690–698 (1955).Google Scholar
  11. Holoubek, V.: Mixed reconstitution between protein from common tobacco mosaic virus and ribonucleic acid from other strains. Virology18, 401–404 (1962).PubMedGoogle Scholar
  12. Nozu, Y., Okada, Y.: Amino acid sequence of a common Japanese strain of tobacco mosaic virus. J. molec. Biol.35, 643–646 (1968).PubMedGoogle Scholar
  13. —, Tochihara, H., Komuro, Y., Okada, Y.: Chemical and immunological properties of cucumber green mottle mosaic virus, watermelon strain. Virology45, 577–585 (1971).PubMedGoogle Scholar
  14. Ohashi, Y., Ohno, T., Nozu, Y., Okada, Y.: Reconstitution of tobacco mosaic virus in vitro. Inhibition by coat proteins of other strains. Proc. Japan Acad.45, 919–924 (1969).Google Scholar
  15. Ohno, T., Nozu, Y., Okada, Y.: Polar reconstitution of tobacco mosaic virus. Virology44, 510–516 (1971).PubMedGoogle Scholar
  16. Okada, Y., Ohashi, Y., Ohno, T., Nozu, Y.: Sequencial reconstitution of tobacco mosaic virus. Virology42, 243–245 (1970).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Yoshimi Okada
    • 1
  • Takeshi Ohno
    • 1
  1. 1.Institute for Plant Virus ResearchChibaJapan

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