Skip to main content
SpringerLink
Log in

Renal vasodilation to histaminein vitro: Roles of nitric oxide, cyclo-oxygenase products and H2 receptors

  • Published:
Inflammation Research Aims and scope Submit manuscript

Abstract

The aim of this study was to evaluate the roles of nitric oxide (NO) and prostanoids in vasodilation to histamine in the preconstricted isolated perfused rat kidney. Kidneys were excised from Hypnorm/Hypnovelanaesthetised Wistar rats and perfused at constant flowin vitro. Renal perfusion pressure was elevated similarly with methoxamine (3 µM) or modified Krebs Henseleit solution containing high KCl (30 mM) and vasodilation to histamine (10, 30 nmol) and papaverine (30, 100 nmol) was then examined before and during perfusion with the NO synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME, 0.3 mM) or the cyclo-oxygenase inhibitor, indomethacin (10 µM). Furthermore, the vasodilator response to 30 nmol histamine was examined in the presence of the H2 receptor antagonist, ranitidine (0.1–10 µM). Vasodilation to histamine (10, 30 nmol) was found to be unaffected by L-NAME (0.3 mM) or indomethacin (10 µM), while ranitidine (0.1–10 µM) antagonised vasodilation to 30 nmol histamine with an estimated pA2 of 6.67. Vasodilation to histamine in the isolated perfused rat kidney is therefore probably independent of NO and prostanoids and mediated by H2 receptors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Conger JD. Vascular abnormalities in the maintenance of acute renal failure. Circ Shock 1983;11:235–44.

    PubMed  Google Scholar 

  2. Conger JD, Robinette JB, Hammond WS. Differences in vascular reactivity in models of ischaemic acute renal failure. Kidney Int 1991;39:1087–97.

    PubMed  Google Scholar 

  3. Conger JD, Robinette JB, Schrier RW. Smooth muscle calcium and endothelium-derived relaxing factor in the abnormal vascular responses of acute renal failure. J Clin Invest 1988;82:532–7.

    PubMed  Google Scholar 

  4. Matthys E, Patton KMK, Osgood RW, Venkatachalam MA, Stein JH. Alterations in vascular function and morphology in acute ischaemic failure. Kidney Int 1983;23:717–24.

    PubMed  Google Scholar 

  5. Furchgott RF, Zawadzki JV. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 1980;288:373–6.

    PubMed  Google Scholar 

  6. Bhardwaj R, Moore PK. Endothelium-derived relaxing factor and the effects of acetylcholine and histamine on resistance blood vessels. Br J Pharmacol 1988;95:835–43.

    PubMed  Google Scholar 

  7. Palmer RMJ, Ferrige AG, Moncada S. Nitric oxide release accounts for the biologic activity of endothelium-derived relaxing factor. Nature 1987;333:524–6.

    Google Scholar 

  8. Palmer RMJ, Rees DD, Ashton DS, Moncada S. L-arginine is the physiological precursor of the formation of nitric oxide in endothelium-dependent relaxation. Biochem Biophys Res Comm 1988;153:1251–6.

    PubMed  Google Scholar 

  9. Rees DD, Palmer RMJ, Schulz R, Hodson HF, Moncada S. Characterisation of three inhibitors of nitric-oxide synthase in vitro and in vivo. Br J Pharmacol 1990;101:746–52.

    PubMed  Google Scholar 

  10. Baenziger NL, Fogerty FJ, Mertz LF, Chernuta LF. Regulation of histamine-mediated prostacyclin synthesis in cultured human vascular endothelial cells. Cell 1981;24:915–23.

    PubMed  Google Scholar 

  11. McIntyre TM, Zimmerman GA, Satoh K, Prescott M. Cultured endothelial cells synthesize both platelet-activating factor and prostacyclin in response to histamine, bradykinin, and adenosine triphosphate. J Clin Invest 1985;76:271–9.

    PubMed  Google Scholar 

  12. Tenner TE, McCully JP. Characterisation of H2-receptor mediated relaxation in rabbit aortic strips. Eur J Pharmacol 1981;73:293–300.

    Google Scholar 

  13. Casteels R, Suzuki H. The effect of histamine on the smooth muscle cells of the ear artery of the rabbit. Pflügers Arch 1980;387:17–25.

    Google Scholar 

  14. Ercan ZS, Türker RK. Histamine receptors in the isolated rat stomach fundus and rabbit aortic strips. Pharmacol 1977;15:118–26.

    Google Scholar 

  15. Mirbahar KB, Eyre P. Occurrence of inhibitory histamine H2-receptors in bovine pulmonary arteries. Eur J Pharmacol 1982;77:331–4.

    PubMed  Google Scholar 

  16. Nishiitsutsuji-Uwo JM, Ross BD, Krebs HA. Metabolic activities of the isolated perfused rat kidney. Biochem J 1967;103:852–62.

    PubMed  Google Scholar 

  17. Schurek HF, Brecht JP, Lohfert H, Hierholzer K. The basic requirements for the function of the isolated cell free perfused rat kidney. Pflügers Arch 1975;354:349–65.

    Google Scholar 

  18. Linas SL. Mechanism of hyperreninaemia in the potassium-depleted rat. J Clin Invest 1981;68:347–55.

    PubMed  Google Scholar 

  19. Burton GA, MacNeil S, De Jonge A, Haylor J. cGMP release and vasodilation induced by EDRF and ANF in the isolated perfused kidney of the rat. Br J Pharmacol 1990;99:364–8.

    PubMed  Google Scholar 

  20. Lazareno S, Birdsall NJM. Estimation of competitive antagonist affinity from functional inhibition curves using the Gaddum, Schild and Cheng-Prusoff equations. Br J Pharmacol 1993;109:1110–9.

    PubMed  Google Scholar 

  21. Lazareno S, Birdsall NJM. Estimation of antagonist K b from inhibition curves in functional experiments: alternatives to the Cheng-Prusoff equation. Trends Pharmacol Sci 1993;14:237–9.

    PubMed  Google Scholar 

  22. Balfagon G, Galvan R, Marco EJ. Mechanism of the indirect adrenergic effect of histamine in cat cerebral arteries. J Pharm Pharmacol 1984;36:248–52.

    PubMed  Google Scholar 

  23. Adeagbo AS, Malik KU. Endothelium-dependent and BR34915-induced vasodilation in rat isolated perfused mesenteric arteries: role of G-proteins, potassium and calcium currents. Br J Pharmacol 1990;100:427–34.

    PubMed  Google Scholar 

  24. Molleman A, Hoiting B, Duin M, van den Anker J, Melemans A, Den Hertog A. Potassium channels regulated by inositol 1,3,4,5-tetrakisphosphate and internal calcium in DDTI MF-2 smooth muscle cells. J Biol Chem 1991;266:5658–63.

    PubMed  Google Scholar 

  25. Neliat G, Masson F, Gargouil YM. Modulation of the spontaneous transient outward currents by histamine in single vascular smooth muscle cells. Pflugers Arch 1989;414:S186–7.

    PubMed  Google Scholar 

  26. Welch WJ, Wilcox CS, Aisaka K, Gross SS, Griffith OW, Fontoura BMA, Maack T, Levi R. Nitric oxide synthesis from L-arginine modulates renal vascular resistance in isolated perfused and intact rat kidneys. J Cardiovasc Pharmacol 1991;17(S3):S165–8.

    PubMed  Google Scholar 

  27. Radermacher J, Fürstermann U, Frülich C. Endotheliumderived relaxing factor influences renal vascular resistance. Am J Physiol 1992;259:F9–17.

    Google Scholar 

  28. Tolins JP, Palmer RMJ, Moncada S, Raij L. Role of endothelium-derived relaxing factor in regulation of renal haemodynamic responses. Am J Physiol 1990;258:H665–2.

    Google Scholar 

  29. Gulbins E, Hoffend J, Zou AP, Dietrich MS, Schlottmann K, Cavarape A, Steinhausen M. Endothelin and endotheliumderived relaxing factor control of basal renovascular tone in hydronephrotic rat kidneys. J Physiol 1993;469:571–82.

    PubMed  Google Scholar 

  30. Hoffend J, Cavarape A, Endlich K, Steinhausen M. Influence of endothelium-derived relaxing factor on renal microvessels and pressure-dependent vasodilation. Am J Physiol 1993;265:F285–92.

    PubMed  Google Scholar 

  31. Farhat MY, Ramwell PW, Thomas G. Endothelium-mediated effects of N-substituted arginines on the isolated perfused rat kidney. J Pharmac Exp Therap 1990;255:473–7.

    Google Scholar 

  32. Reinhardt D, Ritter E. Hypothermia-induced potentiation of histamine H2-receptor-mediated relaxation and cAMP increases in the isolated mesenteric artery of the rabbit. Agents Actions 1979;9:9–14.

    PubMed  Google Scholar 

  33. Cavanagh RL, Usakewicz JJ, Buyniski JP. A comparison of some of the pharmacological properties of etintidine, a new histamine H2-receptor antagonist, with those of cimetidine, ranitidine and tiotidine. J Pharmac Exp Therap 1983;224: 171–9.

    Google Scholar 

  34. Akar F, Ercan ZS, Türker RK. A marked H1-receptor-mediated vasodilator effect of histamine in the isolated perfused rat-heart. Eur J Pharmacol 1984;97:265–9.

    PubMed  Google Scholar 

  35. Moritoki H, Hosoki E, Ishida Y. Age-related decrease in endothelium-dependent dilator response to histamine in rat mesenteric artery. Eur J Pharmacol 1986;126:61–7.

    PubMed  Google Scholar 

  36. van de Voorde J, Leusen I. Influence of prostaglandin-synthesis inhibitors on carbachol- and histamine-induced vasodilation in perfused rat hindquarters. Plügers Arch 1983;397:290–4.

    Google Scholar 

  37. Arrang JM, Garbag M, Lancelot JC, Lecomte JM, Pollard H, Robba M, Schunack W, Schwartz JC. Highly potent and selective ligands for histamine H3-receptors. Nature 1987;327:117–22.

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. D. W. Laight

    Present address: William Harvey Research Institute, Charterhouse Square, EC1M 6BQ, London, UK

Authors and Affiliations

  1. Hoffman La-Roche Inc., 340 Kingsland St., 07110-1199, New Jersey, USA

    J. F. Waterfall

  2. Pharmacology Group, Bath University, Claverton Down, BA2 7AY, Bath, England

    D. W. Laight & B. Woodward

Authors
  1. D. W. Laight
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Woodward
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. F. Waterfall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Laight, D.W., Woodward, B. & Waterfall, J.F. Renal vasodilation to histaminein vitro: Roles of nitric oxide, cyclo-oxygenase products and H2 receptors. Inflamm Res 44, 116–120 (1995). https://doi.org/10.1007/BF01782021

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01782021

Key words

Search

Navigation