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Nitric oxide, prostaglandins and angiotensin II in the regulation of renal medullary blood flow during volume expansion

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Abstract

Regulation of medullary blood flow (MBF) is essential in maintaining renal function and blood pressure. However, it is unknown whether outer MBF (OMBF) and papillary blood flow (PBF) are regulated independently when extracellular volume (ECV) is enhanced. The aim of this study was to determine whether OMBF and PBF are differently regulated and whether there is an interaction between nitric oxide (NO), prostaglandins (PGs) and angiotensin II (Ang II) in regulating OMBF and PBF when ECV is enhanced. To achieve these goals, OMBF and PBF were measured by laser-Doppler in volume-expanded rats treated with a cyclooxygenase inhibitor (meclofenamate, 3 mg/kg) and/or a NO synthesis inhibitor (L-nitro-arginine methyl ester (L-NAME), 3 μg/kg/min) and/or Ang II (10 ng/kg/min). OMBF was unchanged by NO or PGs synthesis inhibition but decreased by 36 % (P < 0.05) when L-NAME and meclofenamate were infused simultaneously. PBF was similarly reduced by L-NAME (12 %), meclofenamate (17 %) or L-NAME + meclofenamate (19 %). Ang II did not modify OMBF, but it led to a similar decrease (P < 0.05) in OMBF when it was administered to rats with reduced NO (32 %), PGs (36 %) or NO and PGs (37 %) synthesis. In contrast, the fall in PBF induced by Ang II (12 %) was enhanced (P < 0.05) by the simultaneous PGs (30 %) or PGs and NO (31 %) synthesis inhibition but not in L-NAME-treated rats (20 %). This study presents novel findings suggesting that blood flows to the outer medulla and renal papilla are differently regulated and showing that there is a complex interaction between NO, PGs and Ang II in regulating OMBF and PBF when ECV is enhanced.

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Acknowledgments

This study was supported by a grant from the Dirección General de Investigación Científica y Técnica of Ministerio de Economía y Competitividad (BFU2013-49098R).

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, University of Murcia, Murcia, 30100, Spain

    María T. Llinás, Francisca Rodriguez, Juan M. Moreno & F. Javier Salazar

  2. Regional Campus of International Excellence “Mare Nostrum”, Murcia, Spain

    María T. Llinás, Francisca Rodriguez, Juan M. Moreno & F. Javier Salazar

  3. Instituto Murciano de Investigación Biomédica, Murcia, Spain

    María T. Llinás, Francisca Rodriguez, Juan M. Moreno & F. Javier Salazar

  4. Cardiovascular and Metabolic Diseases, MedImmune, Cambridge, UK

    Carol Moreno

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  1. Carol Moreno
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  2. María T. Llinás
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  3. Francisca Rodriguez
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  4. Juan M. Moreno
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  5. F. Javier Salazar
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Correspondence to F. Javier Salazar.

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Moreno, C., Llinás, M.T., Rodriguez, F. et al. Nitric oxide, prostaglandins and angiotensin II in the regulation of renal medullary blood flow during volume expansion. J Physiol Biochem 72, 1–8 (2016). https://doi.org/10.1007/s13105-015-0450-8

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  • DOI: https://doi.org/10.1007/s13105-015-0450-8

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