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Quantification of metabolites from single-voxelin vivo 1H NMR data of normal human brain by means of time-domain data analysis

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Abstract

We present here a combination of time-domain signal analysis procedures for quantification of human brainin vivo 1H NMR spectroscopy (MRS) data. The method is based on a separate removal of a residual water resonance followed by a frequency-selective time-domain line-shape fitting analysis of metabolite signals. Calculation of absolute metabolite concentrations was based on the internal water concentration as a reference. The estimated average metabolite concentrations acquired from six regions of normal human brain with a single-voxel spin-echo technique for theN-acetylaspartate, creatine, and choline-containing compounds were 11.4±1.0,6.5±0.5, and 1.7±0.2 mmol kg−1 wet weight, respectively. The time-domain analyses ofin vivo 1H MRS data from different brain regions with their specific characteristics demonstrate a case in which the use of frequency-domain methods pose serious difficulties.

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Authors and Affiliations

  1. Department of Physical Sciences, NMR Research Group University of Oulu, Oulu, Finland

    Mika Ala-Korpela, Jyrki Keisala & Jukka Jokisaari

  2. Hammersmith Hospital, MR Unit, London, UK

    Mika Ala-Korpela

  3. Department of Clinical Radiology, University Hospital of Kuopio, Kuopio, Finland

    Jussi-Pekka Usenius, Pauli Vainio & Seppo Soimakallio

  4. Division of Biochemistry, CRC Biomedical Magnetic Resonance Research Group, St. George's Hospital Medical School, London, UK

    Aad van den Boogaart

  5. NMR Research Group, Department of Biochemistry and Biotechnology, A.I. Virtanen Institute, University of Kuopio, FIN-70211, Kuopio, Finland

    Jussi-Pekka Usenius & Risto Kauppinen

Authors
  1. Mika Ala-Korpela
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  2. Jussi-Pekka Usenius
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  3. Jyrki Keisala
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  4. Aad van den Boogaart
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  5. Pauli Vainio
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  6. Jukka Jokisaari
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  7. Seppo Soimakallio
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  8. Risto Kauppinen
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Ala-Korpela, M., Usenius, JP., Keisala, J. et al. Quantification of metabolites from single-voxelin vivo 1H NMR data of normal human brain by means of time-domain data analysis. MAGMA 3, 129–136 (1995). https://doi.org/10.1007/BF01771697

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  • DOI: https://doi.org/10.1007/BF01771697

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