Skip to main content
SpringerLink
Log in

The principle of determining relative enzyme activities by comparative kinetic microphotometryin situ

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The principles by which the relative activities of enzymesin situ can be determined by microphotometry are reviewed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • ALTMAN, F. P. (1980) Tissue stabilizer methods in histochemistry. InTrends in Enzyme Histochemistry and Cytochemistry, Ciba Foundation Symposium 73, pp. 81–101. Amsterdam, Oxford, New York: Excerpta Medica.

    Google Scholar 

  • BITENSKY, L. (1980) Microdensitometry. InTrends in Enzyme Histochemistry and Cytochemistry, Ciba Foundation Symposium 73, pp. 181–202. Amsterdam, Oxford, New York: Excerpta Medica.

    Google Scholar 

  • BRANDAU, H. & PETTE, D. (1966) Topische Muster von Enzymen des energieliefernden Stoffwechsels im quergestreiften Muskel.Enzym. Biol. Clin. 6, 123–56.

    Google Scholar 

  • BUCHEGGER, A., NEMETH, P. M., PETTE, D. & REICHMANN, H. (1984) Effects of chronic stimulation on the metabolic heterogeneity of the fibre population in rabbit tibialis anterior muscle.J. Physiol. 350, 109–19.

    Google Scholar 

  • DANGAIN, J., PETTE, D. & VRBOVA, G. (1987) Developmental changes in succinate dehydrogenase activity in muscle fibers from normal and dystrophic mice.Exp. Neurol. 95, 224–34.

    Google Scholar 

  • GOLDSTEIN, D. J. (1981) Errors in microdensitometry.Histochem. J. 13, 251–67.

    Google Scholar 

  • LOWRY, O. H. & PASSONNEAU, J. V. (1972)A Flexible System of Enzymatic Analysis. New York, London: Academic Press.

    Google Scholar 

  • NEMETH, P. M. & PETTE, D. (1981) Succinate dehydrogenase activity in fibres classified by myosin ATPase in three hindlimb muscles of rat.J. Physiol. 320, 73–80.

    Google Scholar 

  • NOLTE, J. & PETTE, D. (1972) Microphotometric determination of enzyme activity in single cells in cryostat sections. I. Application of the gel film technique to microphotometry and studies on the intralobular distribution of succinate dehydrogenase and lactate dehydrogenase activities in rat liver.J. Histochem. Cytochem. 20, 567–76.

    Google Scholar 

  • PETTE, D. (1981) Microphotometric measurement of initial maximum reaction rates in quantitative enzyme histochemistryin situ.Histochem. J. 13, 319–27.

    Google Scholar 

  • PETTE, D. & TYLER, K. R. (1983) Response of succinate dehydrogenase activity in fibres of rabbit tibialis anterior muscle to chronic nerve stimulation.J. Physiol. 338, 1–9.

    Google Scholar 

  • PETTE, D., WASMUND, H. & WIMMER, M. (1979) Principle and method of kinetic microphotometric enzyme activity determinationin situ.Histochemistry 64, 1–10.

    Google Scholar 

  • PETTE, D. & WIMMER, M. (1979) Kinetic microphotometric activity determination in enzyme containing gels and model studies with tissue sections.Histochemistry 64, 11–22.

    Google Scholar 

  • PETTE, D. & WIMMER, M. (1980) Microphotometric determination of enzyme activities in cryostat sections by the gel film technique. InTrends in Enzyme Histochemistry and Cytochemistry, Ciba Foundation Symposium 73, pp. 121–34. Amsterdam, Oxford, New York: Excerpta Medica.

    Google Scholar 

  • REICHMANN, H. (1988) Enzyme activity measured in single muscle fibers in partial cytochrome c oxidase deficiency.Neurology 38, 244–9.

    Google Scholar 

  • REICHMANN, H. & PETTE, D. (1982) A comparative microphotometric study of succinate dehydrogenase activity levels in type I, IIA, and IIB fibres of mammalian and human muscles.Histochemistry 74, 27–41.

    Google Scholar 

  • PETTE, D. & WASMUND, H. (1990) Kinetic measurement of cellular enzyme activities in tissue sections with the use of a specifically designed image analysis system.Histochemistry, in preparation.

  • REICHMANN, H. & PETTE, D. (1984a) Glycerolphosphate oxidase and succinate dehydrogenase activities in IIA and IIB fibres of mouse and rabbit tibialis anterior muscles.Histochemistry 80, 429–33.

    Google Scholar 

  • REICHMANN, H. & PETTE, D. (1984b) Enzyme activities and activity profiles in muscle fibres of dystrophic, immature-normal, and adult-normal B16 mice.Muscle Nerve 7, 121–6.

    Google Scholar 

  • STOWARD, P. J. (1980) Criteria for the validation of quantitative histochemical enzyme techniques. InTrends in Enzyme Histochemistry and Cytochemistry, Ciba Foundation Symposium 73, pp. 11–31. Amsterdam, Oxford, New York: Excerpta Medica.

    Google Scholar 

  • VAN NOORDEN, C. J. F. (1988) Enzyme reaction rate studies in tissue sections.Proc. Roy. Microscop. Soc. 23, 93–7.

    Google Scholar 

  • VAN NOORDEN, C. J. F. & BUTCHER, R. G. (1987) Linearity in dehydrogenase reaction rate studies in tissue sections is affected by loss of endogenous substrates during the reaction.J. Histochem. Cytochem. 35, 1401–4.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750, Konstanz, Germany

    D. Pette

  2. Neurologische Universitätsklinik, Würzburg, Germany

    H. Reichmann

Authors
  1. D. Pette
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Reichmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pette, D., Reichmann, H. The principle of determining relative enzyme activities by comparative kinetic microphotometryin situ . Histochem J 21, 531–534 (1989). https://doi.org/10.1007/BF01753353

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01753353

Keywords

Search

Navigation