Summary
Two procedures to detect and purify covalently closed circular plasmid DNA present inThermus strains from Icelandic hot springs are described in detail. Using these procedures, plasmid DNA was detected in IcelandicThermus strains B1 and B2, and 0.2–0.4 mg of pure plasmid DNA was isolated from a litre of the cultures (8.5–9.0 g wet weight). Strain B1 harboured one plasmid species, pCLK 1; strain B2 harboured two plasmid species, pCLK 2 and pCLK 3.
Résumé
Deux procédés pour la détection et la purification d'ADN plasmidiques circulaires de souches deThermus provenant de sources thermales islandaises sont décrits de façon détaillée. Par ces procédés, de l'ADN plasmidique a été décelé dans les souches islandaises deThermus Bl et B2, et 0.2–0.4 mg d'ADN plasmidique pur a été isolé à partir d'un litre de culture (8.5–9.0 g, poids humide). La souche Bl héberge une suele éspèce de plasmide (pCLK 1); la souche B2 héberge deux éspèces de plasmides (pCLK 2 et pCLK 3).
Resumen
Se describen detalladamente los procedimientos para detectar y purificar DNA-plásmidos covalentemente cerrados que se hallan en cepas deThermus aisladas de manantiales calientes de Islandia. Usando la metodología descrita se detectó DNA plásmico en las cepas islandesas deThermus B1 y B2. Se aisló 0.2–0.4 mg de DNA plásmico puro a partir de un litro de cultivo (8.5–9.0 g de peso fresco). La cepa B1 contenia una especie de plásmido, pCLK 1; mientras que la cepa B2 contenia dos, pCLK 2 y pCLK 3.
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Koh, CL. Detection and purification of plasmids present inThermus strains from Icelandic hot springs. World J Microbiol Biotechnol 1, 77–81 (1985). https://doi.org/10.1007/BF01748156
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DOI: https://doi.org/10.1007/BF01748156