Cancer Immunology, Immunotherapy

, Volume 30, Issue 5, pp 300–306 | Cite as

Tumour-associated proliferative responses in vitro of regional lymph nodes draining solid cancers in man

  • T. Mainou-Fowler
  • O. Eremin
Original articles


The proliferative responses in vitro of tumourdraining lymph node lymphocytes were evaluated against autologous colon and lung carcinoma cells. The reactivity of lymphocytes appeared to be directed against tumour-associated rather than tumour-specific antigens. The lymphocyte reactivity detected was not due to an autologous mixed lymphocyte reaction. Recombinant interleukin-2 augmented the responses detected but not their tumour specificity. Phenotypic characterisation indicated the presence of T suppressor/cytotoxic (TS/C) cells as well as natural killer (NK) cells. Only the latter, however, were active in functional cytotoxicity assays. The inability to generate both tumour-specific proliferation of tumour-draining lymph node lymphocytes and tumour-specific cytotoxic killer cells may be due to the presence of suppressor cells in the regional lymph nodes; preliminary data suggest the presence of such cells.


Carcinoma Lymph Node Carcinoma Cell Natural Killer Killer Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Coombs RRA, Wilson AB, Eremin O, Gurner BW, Haegert DG, Lawson YA, Bright S, Munro AJ (1977). Comparison of the direct antiglobulin rosetting reaction with the mixed antiglobulin rosetting reaction for the detection of immunoglobulin on lymphocytes. J Immunol Methods 18: 45PubMedGoogle Scholar
  2. 2.
    Cordell IL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Dulforel H, Stein H, Mason DV (1984) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAPP) complexes. J Histochem Cytochem 32: 219PubMedGoogle Scholar
  3. 3.
    Eremin O, Plumb D, Coombs RRA (1976) T and B lymphocyte populations in human lymph node, regional tumour lymph node and inflammatory lymph node. Int Arch Allergy Appl Immunol 52: 277PubMedGoogle Scholar
  4. 4.
    Eremin O, Roberts RH, Plumb O, Stephens JP (1980) Human regional tumour lymph nodes: alterations of microarchitecture and lymphocyte subpopulations. Br J Cancer 41: 62PubMedGoogle Scholar
  5. 5.
    Eremin O, Coombs RRA, Ashby J (1981) Lymphocytes infiltrating human breast cancers lack K cell activity and show how levels of NK cell activity. Br J Cancer 44: 166PubMedGoogle Scholar
  6. 6.
    Eremin O, Coombs RRA, Prospero TD, Plumb D (1982) T lymphocytes and B lymphocyte subpopulations infiltrating human mammary carcinomas. J Natl Cancer Inst 69: 1PubMedGoogle Scholar
  7. 7.
    Grimm EA, Vose BM, Chu EW, Wilson DJ, Lotze MT, Rayner AA, Rosenberg SA (1984) The human mixed lymphocyte-tumour cell interaction test. I. Positive autologous lymphocyte proliferative responses can be stimulated by tumour cells as well as by cells from normal tissue. Cancer Immunol Immunother 17: 83PubMedGoogle Scholar
  8. 8.
    Hardt C, Diamantstein T, Wagner H (1985) Signal requirements for the in vitro differentiation of cytotoxic T lymphocytes (CTL): distinct soluble mediators promote preactivation of CTL-precursors, clonal growth and differentiation into cytotoxic effects and cells. Eur J Immunol 15: 472PubMedGoogle Scholar
  9. 9.
    Ichino Y, Ishikawa T (1984) Generation of human cytotoxic T lymphocytes against fresh autologous and allogeneic solid tumors by mixed lymphocyte tumour cell culture with T cell growth factor. Jpn J Cancer Res (Gann) 75: 436Google Scholar
  10. 10.
    Kadoyama C, Kimura H, Yamaguchi Y (1987) Inhibition of cytotoxicity to autologous tumour cells by the regional lymph node cells of patients with primary lung cancers. Jpn J Clin Oncol 17: 19PubMedGoogle Scholar
  11. 11.
    Kimura H, Yamaguchi Y, Fujisawa T (1984) Cytotoxicity of autologous and allogeneic lymphocytes against cultured human lung cancer cells: optimal conditions for the production of cytotoxic lymphocytes. Jpn J Cancer Res (Gann) 75: 1006Google Scholar
  12. 12.
    Kuppner M, Wilkinson S, Casson E, Eremin O (1987) In vitro generation of tumour-specific lymphocyte reactivity to colonic carcinoma cells. Comparison with normal colonic mucosa cells. Cancer Immunol Immunother 25: 209PubMedGoogle Scholar
  13. 13.
    MacDermott RP, Stacey MC (1981) Further characterisation of human autologous mixed leucocyte reaction (MLR). J Immunol 12: 729Google Scholar
  14. 14.
    Mavligit GM, Gutterman JU, McBride CM, Hersh EM (1973) Cell mediated immunity to human solid tumour. In vitro detection by lymphocyte blastogenic responses to cell-associated and solubilised tumour antigens. Natl Cancer Inst Monogr 37: 167PubMedGoogle Scholar
  15. 15.
    Mills CD, North RJ (1985) Lyt1+2 suppressor T cells inhibit the expression of passively transferred antitumour immunity by suppressing the generation of cytotoxic T cells. Transplantation 39: 202PubMedGoogle Scholar
  16. 16.
    Slovin SF, Lackman RD, Ferrone S, Kiely PE, Mastrangelo MJ (1986) Cellular immune response to human sarcomas: cytotoxic T cell clones reactive with autologous sarcomas. I. Development, phenotype and specificity. J Immunol 137: 3042PubMedGoogle Scholar
  17. 17.
    Uchida A, Moore M (1985) Lysis of fresh human tumour cells by autologous tumour-associated lymphocytes: two distinct types of autologous tumour Killer cells induced by co-culture with autologous tumour. Cancer Immunol Immunother 20: 29PubMedGoogle Scholar
  18. 18.
    Uchida A, Moore M, Klein E (1987) Autologous mixed lymphocyte-tumour reaction and autologous mixed lymphocyte reaction. I. Proliferation of two distinct T cell subsets. Int J Cancer 40: 165PubMedGoogle Scholar
  19. 19.
    Vanky FT, Stjernsward J (1979) Lymphocyte stimulation (by autologous tumour biopsy cells). In: Herberman RE, McIntire KR (eds) Immunodiagnosis of cancer, part. 2. Marcel Dekker Inc, New York, pp 998Google Scholar
  20. 20.
    Vanky FT, Stjernsward J, Nilsonne U, Sundblad R (1973) Differences in the tumour-associated reactivity of blood lymphocytes and draining lymph node cells in sacroma patients. J Natl Cancer Inst 51: 17PubMedGoogle Scholar
  21. 21.
    Vose BM, Bonnard GD (1982) Human tumour antigens defined by cytotoxicity and proliferative responses of cultured lymphoid cells. Nature 296: 359PubMedGoogle Scholar
  22. 22.
    Vose BM, Howell A (1983) Cultured human anti-tumour T cells and their potential for therapy. In: Herberman RE (ed) Basic and clinical tumour immunology. Martinus Nijhoff, Boston, Mass, pp 129Google Scholar
  23. 23.
    Vose BM, Vanky FT, Klein E (1977) Human tumour-lymphocyte interaction in vitro. V. Comparison of the reactivity of tumour-infiltrating, blood and lymph node lymphocytes with autologous tumour cells. Int J Cancer 20: 895PubMedGoogle Scholar
  24. 24.
    Vose BM, Gallagher P, Moore M, Schofield PF (1981) Specific and non-specific lymphocyte cytotoxicity in colon carcinoma. Br J Cancer 44: 846PubMedGoogle Scholar
  25. 25.
    Vose BM, Ferguson R, Moore M (1982) Mitogen responsiveness and inhibitory activity of mesenteric lymph node cells. Conditioned medium containing T cell growth factor reverses suppressor function. Cancer Immunol Immunother 13: 105PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • T. Mainou-Fowler
    • 1
  • O. Eremin
    • 1
  1. 1.Department of SurgeryUniversity of Aberdeen, University Medical BuildingsForesterhill, AberdeenUK

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