Skip to main content
SpringerLink
Log in

Polyribosomes and messenger RNA from RAT liver mitochondria

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Rat liver mitochondrial polyribosomes were isolated free from cytoplasmic ribonucleoprotein contaminations in a number of criteria (sedimentation and buoyant density patterns, ribosomal RNA composition). Heterogeneous poly A containing RNA from mitochondrial polysomes was purified by two-stage cellulose chromatography. This RNA was in vitro labelled with125I up to specific activity ~106–107 cts.min−1.µg −1 and used for hybridization experiments with separate complementary strands of mitochondrial DNA and nuclear DNA fragments. The proportions of mitochondrial poly A containing RNA that is complementary to heavy and light strands of mtDNA were respectively 31.5% and 8.3%. Besides, a significant RNA fraction was complementary to unique sequences of nuclear DNA (2–3 copies per haploid genome). The hybrids that were formed possessed a high Tm indicative of a perfect base pairing. A dual intracellular origin of mitochondrial messenger RNA is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ojala, D. and Attardi, G., 1972. J. Mol. Biol. 65, 273–290.

    Google Scholar 

  2. Perlman, S., Abelson, H. T. and Penman, S., 1973. Proc. Nat. Acad. Sci. USA, 70, 350–353.

    Google Scholar 

  3. Gaitskhoki, V. S., Kisselev, O. I. and Klimov, N. A., 1973. FEBS Letters 37, 260–263.

    Google Scholar 

  4. Avadhani, N. C., Battula, N. and Rutman, R. J., 1973. Biochemistry, 12, 4122–4129.

    Google Scholar 

  5. Ojala, D. and Attardi, G., 1974. J. Mol. Biol., 88, 205–221.

    Google Scholar 

  6. Malkin, L. I., 1971. Biochemistry 10, 4752–4756.

    Google Scholar 

  7. Spirin, A. S., Belitsina, N. V. and Lerman, M. I., 1965. J Mol. Biol. 11, 611–615.

    Google Scholar 

  8. Perry, R., LaTorre, J., Kelley, D. E. and Greenberg, J. R., 1972. Biochim. Biophys. Acta 262, 220–226.

    Google Scholar 

  9. Schutz, G., Beato, M. and Feigelson, N., 1972. Biochem. Biophys. Res. Communs 49, 680–684.

    Google Scholar 

  10. Rudloff, R., Bauer, W. and Winograd, J., 1967. Proc. Nat. Acad. Sci. USA 57, 1514–1517.

    Google Scholar 

  11. David, I., 1972. J. Mol. Biol. 63, 207–216.

    Google Scholar 

  12. Scherberg, S. and Refotoff, S., S., 1973. Nature New Biol. 242, 142–144.

    Google Scholar 

  13. Gaitskhoki, V. S., Melnikova, M. P., Rakhimbekova, L. S. and Turovski V. S., 1969. Biokhimiya 34, 633–642.

    Google Scholar 

  14. Perlman, S. and Penman, S., 1970. Nature 227, 133–138.

    Google Scholar 

  15. Gaitskhoki, V. S., Kisselev, O. I., Klimov, N. A., Monakhov, N. K., Mukha, G. V., Schwortzman, A. L. and Neifakh, S. A., 1974. FEBS Letters 43, 151–154.

    Google Scholar 

  16. Kisselev, O. I., Gaitskhoki, V. S. and Klimov, N. A., 1975. Mol. Biol. Repts 2, 143–149.

    Google Scholar 

  17. Aloni, I. and Attardi, G., 1971. J. Mol. Biol. 55, 271–276.

    Google Scholar 

  18. Bishop, J. O., Pemberton, R. and Baglioni, C., 1972. Nature New Biol. 235, 231–234.

    Google Scholar 

  19. Kellems, R., Allison, V. and Butow, R., 1974. J. Biol. Chem. 249, 3297–3303.

    Google Scholar 

  20. Gaitskhoki, V. S., Kisselev, O. I. and Shaposhnikov, Ja. D., 1973. Molekularnaya Biologia 7, 417–423.

    Google Scholar 

  21. Gaitskhoki, V. S. and Kisselev, O. I., 1974. Molekularnaya Biologia 8, 660–665.

    Google Scholar 

  22. Avadhani, N., Lewis, F. and Rutman, R., 1974. Biochemistry 13, 4638–4645.

    Google Scholar 

  23. Swanson, R., 1971. Nature 231, 31–34.

    Google Scholar 

  24. Gaitskhoki, V. S., Kisselev, O. I. and Neifakh, S. A., 1973. FEBS Letters 31, 93–96.

    Google Scholar 

  25. Kisselev, O. I., Gaitskhoki, V. S. and Neifakh, S. A., 1975. Mol. Cell. Biochem. 6, 149–152.

    Google Scholar 

  26. Dimitriadis, G. J. and Georgatsos, J. G., 1974. FEBS Letters 46, 96–100.

    Google Scholar 

  27. David, I. and Blackler, A., 1972. Develop. Biol. 29, 152–161.

    Google Scholar 

  28. Tabak, H., Borst, P. and Tabak, A., 1973. Biochim. Biophys. Acta, 294, 184–191.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Biochemical Genetics, Institute of Experimental Medicine, Academy of Medical Sciences, 197022, Leningrad, USSR

    V. S. Gaitskhoki, O. I. Kisselev & N. A. Klimov

Authors
  1. V. S. Gaitskhoki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. I. Kisselev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. A. Klimov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gaitskhoki, V.S., Kisselev, O.I. & Klimov, N.A. Polyribosomes and messenger RNA from RAT liver mitochondria. Mol Cell Biochem 14, 101–108 (1977). https://doi.org/10.1007/BF01734172

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01734172

Keywords

Search

Navigation