Summary
Some properties of a submitochondrial cell-free system for protein synthesis are described. The system was prepared from rat liver mitochondria lysed with Triton X-100, and the lysate was characterized by a linear rate of [14C]amino acid incorporation for 15–20 min with subsequent decline in activity. The incorporation reaction was inhibited by chloramphenicol and was in-sensitive to cycloheximide. Poly(U) addition stimulated [14C]phenylalanine incorporation by the preincubated submitochondrial system. Upon the addition of 7.5S mRNA that was iso-lated from mitochondria the major translation product was identified as a hydrophobic poly-peptide which in some properties (solubility in chloroform-methanol mixture) was similar to one of polypeptides synthesized by the sub-mitochondrial system on endogeneous mRNAs.
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References
Constantino, P. and Attardi, G., 1975. J. Mol. Biol. 96, 291–298.
Schatz, G. and Mason, T. L., 1974. Ann. Rev. Biochem. 43, 51–87.
Blossey, H. C. and Küntzel, H., 1972. FEBS Letters 24, 335–338.
Küntzel, H. and Blossey, H. C., 1974. Eur. J. Biochem. 47, 165–171.
Halbreich, A., Franco, A. D., Groudinsky, O., Cosson, J. and Slonimski, P. P., 1975. Biochem. Biophys. Res. Communs 64, 1286–1292.
Tzagoloff, A., Rubin, M. S. and Sierra, M. F., 1973. Biochim. Biophys. Acta 301, 71–104.
Avadhani, N. G., Lynch, M. J. and Buetow, D., 1971. Exp. Cell. Res. 69, 226–228.
Gaitskhoki, V. S., Kisselev, O. I., Klimov, N. A., Monakhov, N. K., Mukha, G. V., Schwartzman, A. L. and Neifakh, S. A., 1974. FEBS Letters 43, 151–154.
Greenwalt, J. W. and Schnaitman, C., 1970. J. Cell Biol. 46, 173–185.
Rustum, J. M., Massaro, E. J. and Barnard, E. A., 1971. Biochemistry 10, 3509–3514.
Peacock, A. C. and Dingman, C. W., 1967. Biochemistry 6, 1818–1824.
Molloy, G. R. and Darnell, J. E., 1973. Biochemistry 12, 2324–2330.
Adelman, M., Sabatini, D. and Blobel, G., 1973. J. Cell Biol. 56, 206–229.
Gaitskhoki V. S., Golubkov, V. I., Grabovskaya, K. B., Kisselev, O. I., Totolyan, A. A. and Neifakh, S. A., 1974. Doklady Akademii Nauk SSSR, 215, 1487–1490.
Kuzela, S., Kolarov, J. and Krempasky, V., 1973. Biochem. Biophys. Res. Communs 54, 9–16.
Sierra, M. F. and Tzagoloff, A., 1973. Proc. Nat. Acad. Sci. USA 70, 3155–3159.
Ojala, D. and Attardi, G., 1974. J. Mol. Biol. 88, 205–219.
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Kisselev, O.I., Gaitskhoki, V.S. & Neifakh, S.A. On the cell-free system of protein synthesis from mammalian mitochondria. Mol Cell Biochem 14, 91–96 (1977). https://doi.org/10.1007/BF01734170
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DOI: https://doi.org/10.1007/BF01734170