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Glycolytic metabolism in cultured cells of the nervous system IV. The effects of thiamine deficiency on thiamine levels, metabolites and thiamine-dependent enzymes of the C-6 glioma and C-1300 neuroblastoma cell lines.

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Summary

C-6 glioma and C-1300 neuroblastoma cells were cultured in thiamine deficient and control media. Thiamine levels, transketolase and pyruvate decarboxylase activities, and high energy phosphate metabolites were all measured in deficient and control cells. Thiamine levels in the deficient cells were found to be below the level of detectability. Pyruvate decarboxylase activity was more susceptible to thiamine deficiency in both cell lines than transketolase. In spite of the large decrease in pyruvate decarboxylase activity, high energy phosphate metabolites were not decreased in either cell line. These data indicate that C-6 glioma and C-1300 neuroblastoma cells have the capacity to maintain normal energy metabolites in the presence of large changes in thiamine levels and thiamine dependent enzyme activity.

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Author notes

  1. Joan P. Schwartz

    Present address: Laboratory of Preclinical Pharmacology, NIMH, St. Elizabeth's Hospital, 20032, Washington, DC, USA

Authors and Affiliations

  1. Section on Cellular Neurochemistry, Laboratory of Neuropathology and Neuroanatomical Sciences, National Institute of Neurological and Communicative Disorders and Stroke, National Institute of Health, 20014, Bethesda, MD, USA

    Joan P. Schwartz

  2. Department of Anatomy, The University of Vermont Medical School, 05401, Burlington, VT, USA

    David W. McCandless

  3. Section on Cellular Neurochemistry, LNNS, NINCDS, NIH, 20014, Bethesda, MD, USA

    David W. McCandless

Authors
  1. Joan P. Schwartz
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  2. David W. McCandless
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Supported in part by USPHS grant AA 01391.

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Schwartz, J.P., McCandless, D.W. Glycolytic metabolism in cultured cells of the nervous system IV. The effects of thiamine deficiency on thiamine levels, metabolites and thiamine-dependent enzymes of the C-6 glioma and C-1300 neuroblastoma cell lines.. Mol Cell Biochem 13, 49–53 (1976). https://doi.org/10.1007/BF01732395

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  • DOI: https://doi.org/10.1007/BF01732395

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