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Some properties of RAT liver mitochondrial RNA polymerase

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Summary

A rapid method suitable for purifying large amounts of mitochondria from rat liver using isopycnic zonal centrifugation is described. The RNA polymerase isolated from the purified mitochrondria was found associated with one peak when resolved by DEAE Sephadex chromatography. The enzyme was next fractionated on a phosphocellulose column followed by glycerol gradient centrifugation. A 600-fold purification was achieved when the enzyme was finally filtered through agarose gel. This final enzyme fraction consisted of one polypeptide chain as shown by polyacrylamide gel electrophoresis profiles. The enzyme has a greater preference for poly [d(A-T)] templates than for rat liver mitochondrial DNA. Inhibition of the enzyme activity required high concentrations of the inhibitors. The resistance of the enzyme toα-amanitin indicated that there was no contamination from nuclear RNA polymerase II. The conclusion is drawn that the mitochondrial RNA polymerase activity is associated with a single polypeptide.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, Department of Natural Sciences, The University of Michigan, 48128, Dearborn, Michigan, USA

    Christopher J. Chetsanga, Jay I. Novetsky & Michael J. Dimino

  2. Department of Research, Sinai Hospital of Detroit, 48235, Detroit, Michigan, USA

    Michael J. Dimino

Authors
  1. Christopher J. Chetsanga
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  2. Jay I. Novetsky
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  3. Michael J. Dimino
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Additional information

This work was supported in part by Institutional Grant IN-4ON to the University of Michigan from the American Cancer Society, Grant 360133 from the Rackham School of Graduate Studies and Grant 121010 from the Institute of Science and Technology of the University of Michigan, Grant 340879 from the Michigan Heart Association, and Grants AMO5474 and RRO5641 from the National Institutes of Health.

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Chetsanga, C.J., Novetsky, J.I. & Dimino, M.J. Some properties of RAT liver mitochondrial RNA polymerase. Mol Cell Biochem 13, 147–156 (1976). https://doi.org/10.1007/BF01731777

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