Skip to main content
SpringerLink
Log in

Spindle membranes and calcium sequestration during meiosis ofDysdercus intermedius (Heteroptera)

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

The fate of intracellular membranes stained by the osmium ferricyanide (OsFeCN) procedure was followed from premeiotic interphase to interkinesis inDysdercus intermedius. During diakinesis the centrioles forming primary cilia attach temporarily with their proximal ends to the nuclear envelope which is stretched from pole to pole. Breakdown of the nuclear envelope is preceded by deep indentations with microtubules from growing asters. Vesicles of smooth endoplasmic reticulum which accumulate gradually in the course of prophase contribute to the ensheathment of the chromosomes with membranes. When the nuclear envelope breaks down, the polar parts of the formerly perinuclear membranes follow the ingrowth of the spindle microtubules towards the cell equator where the seven bivalents are arranged in a circle with the X1X2 sex chromosomes in the centre. The metaphase I spindle thus contains longitudinally oriented membranes between the poles, membranous envelopes around all chromosomes and radial connections from the autosomes to the sex chromosomes in the centre. At anaphase the homologues leave their common sheath and a microtubular stembody surrounded by membranes appears between the receding dyads. In the interkinetic nucleus the gonosomes are separated from the autosomes by a common membranous sheath which may be instrumental in their joint assignment to only one pole in the second meiotic division. Calcium sequestering sites visualized by oxalate precipitation are the Golgi lamellae and vesicles derived from them that surround the whole spindle body.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allan VJ, Kreis TE (1986) A microtubule-binding protein associated with membranes of the Golgi apparatus. J Cell Biol 103:2229–2239

    Google Scholar 

  • Baumann O, Walz B (1989) Topography of Ca2+-sequestering endoplasmic reticulum in photoreceptors and pigmented glial cells in the compound eye of the honeybeen drone. Cell Tissue Res 255:511–522

    Google Scholar 

  • Coulon J, Arsanto JP, Thouveny Y (1986) Striated ciliary rootgolgi association in branchial crown epithelial cells ofOwenia. Visualization of Ca2+-binding sites and ATPase activities. Protoplasma 130:108–119

    Google Scholar 

  • Fabiato A, Fabiato F (1978) Calcium-induced release of calcium from the sarcoplasmic reticulum of skinned cells from adult human, dog, cat, rabbit, rat, and frog hearts and from fetal and new-born rat ventricles. Ann NY Acad Sci 307:491–522

    Google Scholar 

  • Fiskum G (1985) Intracellular levels and distribution of Ca2+ in digitonin permeabilized cells. Cell Calcium 6:25–37

    Google Scholar 

  • Forbes MS, Plantholt BA, Sperelakis N (1977) Cytochemical staining procedures selective for sarcotubular systems of muscle: modifications and applications. J Ultrastruct Res 60:306–327

    Google Scholar 

  • Forer A, Gupta L, Hall TA (1980) Electron probe x-ray microanalysis of calcium and other elements in meiotic spindles, in frozen sections of spermatocytes from crane fly testes. Exp Cell Res 126:217–226

    Google Scholar 

  • Grover AK (1985) Ca-pumps in smooth muscle: one in plasma membrane and another in endoplasmic reticulum. Cell Calcium 6:227–236

    Google Scholar 

  • Hafner M, Petzelt C (1987) Inhibition of mitosis by an antibody to the mitotic calcium transport system. Nature 330:264–266

    Google Scholar 

  • Harris P (1975) The role of membranes in the organization of the mitotic apparatus. Exp Cell Res 94:409–425

    Google Scholar 

  • Hasselbach W (1978) Reversibility of the sarcoplasmic reticulum Ca pump. Biochim Biophys Acta 515:23–53

    Google Scholar 

  • Hepler PK (1980) Membranes in the mitotic apparatus of barley cells. J Cell Biol 86:490–499

    Google Scholar 

  • Hepler PK (1981) The structure of the endoplasmic reticulum revealed by osmium tetroxide-potassium ferricyanide staining. Eur J Cell Biol 26:102–110

    Google Scholar 

  • Hepler PK, Wick SM, Wolniak SM (1981) The structure and role of membranes in the mitotic apparatus. In: Schweiger H-G (ed) Int Cell Biol. Springer-Verlag, Heidelberg

    Google Scholar 

  • Izant JG (1983) The role of calcium ions during mitosis. Calcium participates in the anaphase trigger. Chromosoma 88:1–10

    Google Scholar 

  • Karnovsky MJ (1971) Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. Abstracts Am Soc Cell Biol, New Orleans, p 146

    Google Scholar 

  • Kiehart DP (1981) Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system. J Cell Biol 88:604–617

    Google Scholar 

  • Kiehart DP, Inoué S (1976) Local depolymerization of spindle microtubules by microinjection of calcium ions. J Cell Biol 70:230a

    Google Scholar 

  • Lee C, Chen LB (1988) Dynamic behavior of endoplasmic reticulum in living cells. Cell 54:37–46

    Google Scholar 

  • Mazia D, Petzelt C, Williams RO, Meza I (1972) A Ca2+-activated ATPase in the mitotic apparatus of the sea urchin egg (isolated by a new method). Exp Cell Res 70:325–332

    Google Scholar 

  • McGraw CF, Somlyo AV, Blaustein MP (1980) Localization of calcium in presynaptic nerve terminals. J Cell Biol 85:228–241

    Google Scholar 

  • McIntosh JR, Porter ME (1989) Enzymes for microtubule-dependent motility. J Biol Chem 264:6001–6004

    Google Scholar 

  • Mollenhauer HH, Droleskey RE (1980) Some specific staining reactions of potassium ferricyanide in cells of guinea pig testes. J Ultrastruct Res 72:385–391

    Google Scholar 

  • Motzko D, Ruthmann A (1984) Spindle membranes in mitosis and meiosis of the heteropteran insectDysdercus intermedius. A study of the interrelationship of spindle architecture and the kinetic organization of chromosomes. Eur J Cell Biol 33:205–216

    Google Scholar 

  • Neiss WF (1984) Electron staining of the cell surface coat by osmium-low ferrocyanide. Histochemistry 80:231–242

    Google Scholar 

  • Northover AM (1985) The release of membrane-associated calcium from rabbit neutrophils by fixatives. Implications for the use of antimonate staining to localize calcium. Histochemical J 17:443–452

    Google Scholar 

  • Paweletz N (1974) Elektronenmikroskopische Untersuchungen an frühen Stadien der Mitose bei HeLa-Zellen. Cytobiologie 9:368–390

    Google Scholar 

  • Paweletz N, Lang U (1988) Fine structural studies of early mitotic stages in untreated and nocodazole-treated HeLa cells. Eur J Cell Biol 47:334–345

    Google Scholar 

  • Petzelt C (1972) Ca2+-activated ATPase during the cell cycle of the sea urchinStrongylocentrotus purpuratus. Exp Cell Res 70:333–339

    Google Scholar 

  • Petzelt C (1979) Biochemistry of the mitotic spindle. Int Rev Cytol 60:53–92

    Google Scholar 

  • Petzelt C, Hafner M, Mazia D, Sawin KW (1987) Microtubules and Ca2+-sequestering membranes in the mitotic apparatus, isolated by a new method. Eur J Cell Biol 45:268–273

    Google Scholar 

  • Pickett-Heaps JD, Tippit DH (1980) Light and electron microscopic observations on cell division in two large pennate diatoms,Hantzschia andNitzschia. II. Ultrastructure. Eur J Cell Biol 21:12–27

    Google Scholar 

  • Ruthmann A, Dahlberg R (1976) Pairing and segregation of the sex chromosomes in X1X2-males ofDysdercus intermedius with a note on the kinetic organization of heteropteran chromosomes. Chromosoma 54:89–97

    Google Scholar 

  • Ruthmann A, Permantier Y (1973) Spindel und Kinetochoren in der Mitose und Meiose der BaumwollwanzeDysdercus intermedius (Heteroptera). Chromosoma 41:271–288

    Google Scholar 

  • Salmon ED, Segall RR (1980) Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus). J Cell Biol 86:355–365

    Google Scholar 

  • Schnepf E, Hausmann K, Herth W (1982) The osmium tetroxidepotassium ferrocyanide (OsFeCN) staining technique for electron microscopy: a critical evaluation using ciliates, algae, mosses, and higher plants. Histochemistry 76:261–271

    Google Scholar 

  • Simson JAV, Spicer SS (1975) Selective subcellular localization of cations with variants of the potassium pyroantimonate technique. J Histochem Cytochem 23:575–598

    Google Scholar 

  • Trotta EE, De Meis L (1975) ATP-dependent calcium accumulation in brain microsomes. Enhancement by phosphate and oxalate. Biochim Biophys Acta 394:239–247

    Google Scholar 

  • Ungar F, Piscopo J, Letizia J, Holtzman E (1984) Uptake of calcium by the endoplasmic reticulum of the frog photoreceptor. J Cell Biol 98:1645–1655

    Google Scholar 

  • Walz B (1982a) Ca2+-sequestering smooth endoplasmic reticulum in an invertebrate photoreceptor. II. Its properties as revealed by microphotometric measurements. J Cell Biol 93:849–859

    Google Scholar 

  • Walz B (1982b) Ca2+-sequestering smooth endoplasmic reticulum in an invertebrate photoreceptor. I. Intracellular topography as revealed by OsFeCN staining and in situ Ca2+ accumulation. J Cell Biol 93:839–848

    Google Scholar 

  • Weisenberg RC (1972) Microtubule formation in vitro in solutions containing low calcium concentrations. Science 177:1104–1105

    Google Scholar 

  • White DJ, Mazurkiewicz JE, Barnett RJ (1979) A chemical mechanism for tissue staining by tetroxide-ferrocyanide mixtures. J Histochem Cytochem 27:1084–1091

    Google Scholar 

  • Wick SM, Hepler PK (1980) Localization of Ca2+-containing antimonate precipitates during mitosis. J Cell Biol 86:500–513

    Google Scholar 

  • Wise D, Wolniak SM (1984) A calcium-rich intraspindle membrane system in spermatocytes of wolf spiders. Chromosoma 90:156–161

    Google Scholar 

  • Wolniak SM (1988) The regulation of mitotic spindle function. Biochem Cell Biol 66:490–514

    Google Scholar 

  • Wolniak SM, Hepler PK, Jackson WT (1980) Detection of the membrane calcium distribution mitosis inHaemanthus endosperm with chlorotetracycline. J Cell Biol 87:23–32

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lehrstuhl für Zellmorphologie, Ruhr-Universität Bochum, Postfach 102148, D-4630, Bochum 1, Germany

    D. Motzko & A. Ruthmann

Authors
  1. D. Motzko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Ruthmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Motzko, D., Ruthmann, A. Spindle membranes and calcium sequestration during meiosis ofDysdercus intermedius (Heteroptera). Chromosoma 99, 212–222 (1990). https://doi.org/10.1007/BF01731132

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01731132

Keywords

Search

Navigation