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Annals of Hematology

, Volume 68, Issue 3, pp 125–132 | Cite as

Carbohydrate-dependent binding of human myeloid leukemia cell lines to neoglycoenzymes, matrix-immobilized neoglycoproteins, and bone marrow stromal cell layers

  • S. Gabius
  • R. Wawotzny
  • U. Martin
  • S. Wilholm
  • H. -J. Gabius
Original Article

Summary

The presence of sugar receptors on human myeloid leukemia cells was comparatively assessed by a highly sensitive binding assay, employing a panel of 14 types of neoglycoenzymes (chemically glycosylatedEscherichia coli β-galactosidase). The selected carbohydrate ligands mainly encompass common components of natural glycoconjugates as mono- or disaccharides. The monocytoid cells of the THP-1 line, the very young myeloblasts and the myeloblasts of the lines KG-1a and KG-1, the promyelocytes of the HL-60 line, and the early myeloblasts/erythroblasts of the K-562 line displayed a nonuniform pattern of specific binding with quantitative differences at a fixed, nonsaturating concentration of the probes. Scatchard analysis in four cases corroborated the indication of cell-type-related differences between the various cell lines. To test whether the detectable cellular sugar-binding sites can mediate adhesion to glycoligands, a rather simple model matrix of nitrocellulose-immobilized neoglycoproteins was first used. In comparison to the carbohydrate-free carrier protein significant cell adhesion was observed primarily with neoglycoproteins that exposed galactose,N-acetylgalactosamine,N-acetylglucosamine, mannose, and fucose moieties among the 11 tested types of carbohydrate residue. Subsequently, human bone marrow stromal cell layers were tested as a model matrix with increased levels of physiological relevance and complexity. Mixtures of carbohydrate and neoglycoprotein were employed as inhibitors of an interaction via lectins between the stromal and the tumor cells. The carbohydrate-dependent alterations of this parameter revealed cell-type-associated properties. Tumor cell binding was significantly decreased for not more than two lines with the effective sugars, namelyN-acetylgalactosamine, mannose, fucose, and sialic acid.

Key words

Leukemia Bone marrow Cell adhesion Neoglycoprotein Lectin 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • S. Gabius
    • 1
  • R. Wawotzny
    • 1
  • U. Martin
    • 1
  • S. Wilholm
    • 1
  • H. -J. Gabius
    • 1
  1. 1.Institut für Physiologische Chemie, Tierärztliche FakultätLudwig-Maximilians-UniversitätMunichGermany

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