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Carbohydrate-dependent binding of human myeloid leukemia cell lines to neoglycoenzymes, matrix-immobilized neoglycoproteins, and bone marrow stromal cell layers

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Summary

The presence of sugar receptors on human myeloid leukemia cells was comparatively assessed by a highly sensitive binding assay, employing a panel of 14 types of neoglycoenzymes (chemically glycosylatedEscherichia coli β-galactosidase). The selected carbohydrate ligands mainly encompass common components of natural glycoconjugates as mono- or disaccharides. The monocytoid cells of the THP-1 line, the very young myeloblasts and the myeloblasts of the lines KG-1a and KG-1, the promyelocytes of the HL-60 line, and the early myeloblasts/erythroblasts of the K-562 line displayed a nonuniform pattern of specific binding with quantitative differences at a fixed, nonsaturating concentration of the probes. Scatchard analysis in four cases corroborated the indication of cell-type-related differences between the various cell lines. To test whether the detectable cellular sugar-binding sites can mediate adhesion to glycoligands, a rather simple model matrix of nitrocellulose-immobilized neoglycoproteins was first used. In comparison to the carbohydrate-free carrier protein significant cell adhesion was observed primarily with neoglycoproteins that exposed galactose,N-acetylgalactosamine,N-acetylglucosamine, mannose, and fucose moieties among the 11 tested types of carbohydrate residue. Subsequently, human bone marrow stromal cell layers were tested as a model matrix with increased levels of physiological relevance and complexity. Mixtures of carbohydrate and neoglycoprotein were employed as inhibitors of an interaction via lectins between the stromal and the tumor cells. The carbohydrate-dependent alterations of this parameter revealed cell-type-associated properties. Tumor cell binding was significantly decreased for not more than two lines with the effective sugars, namelyN-acetylgalactosamine, mannose, fucose, and sialic acid.

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References

  1. Allen HJ (1986) Binding specificity of a human leucocyte carbohydrate-binding protein. Immunol Invest 15:379–392

    Google Scholar 

  2. Brandley BK, Schnaar RL (1986) Cell-surface carbohydrates in cell recognition and responses. J Leukocyte Biol 40:97–111

    Google Scholar 

  3. Crocker PR, Morris L, Gordon S (1988) Novel cell surface adhesion receptors involved in interactions between stromal macrophages and hematopoietic cells. J Cell Sci [Suppl] 9:185–206

    Google Scholar 

  4. DiCorletto PE, De La Motte CA (1989) Role of cell surface carbohydrate moieties in monocytic cell adhesion to endothelium in vitro. J Immunol 143:3666–3672

    Google Scholar 

  5. Forbes JT, Bretthauer RK, Oeltmann TN (1981) Mannose-6-, fructose-1-, and fructose-6-phosphates inhibit human natural killer cell-mediated cytotoxicity. Proc Natl Acad Sci USA 78:5797–5801

    Google Scholar 

  6. Fukuda M (1985) Cell surface glycoconjugates as onco-differentiation markers in hematopoietic cells. Biochim Biophys Acta 780:119–150

    Google Scholar 

  7. Gabius HJ (1987) Endogenous lectins in tumors and the immune system. Cancer Invest 5:39–46

    Google Scholar 

  8. Gabius HJ (1991) Detection and functions of mammalian lectins — with emphasis on membrane lectins. Biochim Biophys Acta 1071:1–18

    Google Scholar 

  9. Gabius HJ, Bardosi A (1991) Neoglycoproteins as tools in glycohistochemistry. Progr Histochem Cytochem 22:1–72

    Google Scholar 

  10. Gabius S, Gabius HJ (1990) Sugar receptors of the stromal cell layer in human long-term bone marrow cultures: their presence, modulatory responses to changes in the microenvironment and potential role in cell adhesion. Blut 61:232–239

    Google Scholar 

  11. Gabius HJ, Gabius S (eds) (1993) Lectins and glycobiology. Springer, Berlin Heidelberg New York

    Google Scholar 

  12. Gabius S, Hellmann KP, Hellmann T, Brinck U, Gabius HJ (1989) Neoglycoenzymes: a versatile tool for lectin detection in solid-phase assays and glycohistochemistry. Anal Biochem 182:447–451

    Google Scholar 

  13. Gabius S, Hellmann KP, Ciesiolka T, Nagel GA, Gabius HJ (1989) Lineage- and differentiation-dependent alterations in the expression of receptors for glycoconjugates (lectins) in different human hematopoietic cell lines and low-grade lymphomas. Blut 59:165–170

    Google Scholar 

  14. Gabius S, Schirrmacher V, Franz H, Joshi SS, Gabius HJ (1990) Analysis of cell surface sugar receptor expression by neoglycoenzyme binding and adhesion to plastic-immobilized neoglycoproteins for related weakly and strongly metastatic cell lines of murine tumor model systems. Int J Cancer 46:500–507

    Google Scholar 

  15. Gabius S, Yamazaki N, Hanewacker W, Gabius HJ (1990) Regulation of distribution, amount and ligand affinity of sugar receptors in human colon carcinoma cells by treatment with sodium butyrate, retinoic acid and phorbol ester. Anti-cancer Res 10:1005–1012

    Google Scholar 

  16. Gabius S, Wawotzny R, Wilholm S, Martin U, Wörmann B, Gabius HJ (1993) Adhesion of human lymphoid cell lines to immobilized carbohydrates and to bone-marrow stromal cell layers by surface sugar receptors. Int J Cancer 54:1017–1021

    Google Scholar 

  17. Gahmberg CG, Andersson LC (1982) Surface glycoproteins of malignant human leukocytes. Biochim Biophys Acta 780:119–150

    Google Scholar 

  18. Godsave SF, Harrison FL, Chesterton CJ (1981) Molecular properties of a galaptin and its activity in normal and leukemic erythroid tissue. J Supramolec Struct 17:223–230

    Google Scholar 

  19. Greenberg BR, Woo L, Garewal HS (1988) Effects of bone marrow fibroblastic cells and fibroblastic-conditioned medium on HL-60 and KG-1. Leukemia Res 12:61–66

    Google Scholar 

  20. Grillon C, Monsigny M, Kieda C (1990) Cell surface lectins of human granulocytes: their expression is modulated by mono-nuclear cells and granulocyte/macrophage colony-stimulating factor. Glycobiology 1:33–38

    Google Scholar 

  21. Guarnaccia SP, Schnaar RL (1982) Hepatocyte adhesion to immobilized carbohydrates. I. Sugar recognition is followed by energy-dependent strengthening. J Biol Chem 257:14288–14292

    Google Scholar 

  22. Hakomori SI (1989) Aberrant glycosylation in tumors and tumor-associated carbohydrate antigens. Adv Cancer Res 52:257–331

    Google Scholar 

  23. Hardy CL, Omoto E, Tavassoli M (1990) Measurement of homing receptors on the surface of leukemic and non-leukemic cell lines. Pathobiology 58:179–184

    Google Scholar 

  24. Hoppe CA, Lee YC (1982) Stimulation of mannose-binding activity in the rabbit alveolar macrophage by simple sugars. J Biol Chem 257:12831–12834

    Google Scholar 

  25. Issekutz TB (1992) Lymphocyte homing to sites of inflammation. Curr Opinion Immunol 4:287–293

    Google Scholar 

  26. Janusz MJ, Austen KF, Czop JK (1986) Isolation of soluble yeast β-glucans that inhibit human monocyte phagocytosis mediated by β-glucan receptors. J Immunol 137:3270–3276

    Google Scholar 

  27. Kamenov B, Longenecker BM (1990) Significance of acute leukemia-bone marrow stromal cell adherence and its molecular nature. Period Biol 92:94–95

    Google Scholar 

  28. Kamenov B, Kieran MW, Barrington-Leigh J, Longenecker BM (1984) Homing receptors as functional markers for classification, prognosis, and therapy of leukemias and lymphomas. Proc Soc Exp Biol Med 177:211–219

    Google Scholar 

  29. Kawaguchi K, Kohlenschmidt M, Roseman S, Lee YC (1981) Differential uptake ofd-galactosyl- andd-glucosyl-neoglycoproteins by isolated rat hepatocytes. J Biol Chem 256:2230–2234

    Google Scholar 

  30. Koeffler HP (1983) Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62:709–721

    Google Scholar 

  31. Koeffler HP, Golde DW (1980) Human myeloid leukemia cell lines: a review. Blood 56:344–350

    Google Scholar 

  32. Lehrman MA, Haltiwanger RS, Hill RL (1986) The binding of fucose-containing glycoproteins by hepatic lectins. J Biol Chem 261:7426–7432

    Google Scholar 

  33. Lotan R, Lotan D, Carralero DM (1989) Modulation of galactoside-binding lectins in tumor cells by differentiation-inducing agents. Cancer Lett 48:115–122

    Google Scholar 

  34. Loughrey HC, Choi SL, Cullis PR, Bally MB (1990) Optimized procedures for the coupling of proteins to liposomes. J Immunol Methods 132:25–35

    Google Scholar 

  35. Luikart SD, Maniglia CA, Furcht LT, McCarthy JB, Oegema TR (1990) A heparan sulfate-containing fraction of bone marrow stroma induces maturation of HL-60 cells in vitro. Cancer Res 50:3781–3785

    Google Scholar 

  36. Matsuoka T, Tavassoli M (1989) Purification and partial characterization of membrane-homing receptors in two cloned murine hemopoietic progenitor cell lines. J Biol Chem 264:20193–20198

    Google Scholar 

  37. Monsigny M, Roche AC, Midoux P (1984) Uptake of neoglycoproteins via membrane lectin(s) of L1210 cells evidenced by quantitative flow cytofluorometry and drug targeting. Biol Cell 51:187–196

    Google Scholar 

  38. Nojiri H, Kitagawa S, Nakamura M, Kirito K, Enomoto Y, Saito M (1988) Neolacto-series gangliosides induce granulocytic differentiation of human promyelocytic leukemia cell line HL-60. J Biol Chem 263:7443–7446

    Google Scholar 

  39. Ohkawa H, Harigaya K (1987) Effect of direct cell-to-cell interaction between the KM-102 clonal human marrow stromal cell line and the HL-60 myeloid leukemic cell line on the differentiation and proliferation of the HL-60 line. Cancer Res 47:2879–2882

    Google Scholar 

  40. Ossenkoppele GJ, Denkers I, Wijermans P, Huijgens PC, Nauta JJP, Beelen RJH, Langenhuijsen MMAC (1990) Differentiation induction of HL-60 cells in a long-term bone marrow culture of acute myeloid leukemia. Leukemia Res 14:611–616

    Google Scholar 

  41. Paietta E, Gallagher R, Wiernik PH, Stockert RJ (1988) A membrane-bound lectin responsive to monocytic maturation in the promyelocytic leukemia cell line HL-60. Cancer Res 48:280–287

    Google Scholar 

  42. Reed RG (1990) Spurious cell surface receptors: inadequate correction for saturable, nonspecific binding mimics receptor binding. Anal Biochem 185:160–163

    Google Scholar 

  43. Scatchard G (1949) The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51:660–672

    Google Scholar 

  44. Schölzel C, Löwenberg B (1985) Stimulation of proliferation and differentiation of acute myeloid leukemia cells on a bone marrow stroma in culture. Exp Hematol 13:664–669

    Google Scholar 

  45. Stanford DR, Starkey JR, Magnuson JA (1986) The role of tumor cell surface carbohydrate in experimental metastasis. Int J Cancer 37:435–444

    Google Scholar 

  46. Takata I, Chida K, Gordon MR, Myrvik QN, Ricardo MJ, Jucera LS (1987) L-Fucose, D-mannose, L-galactose and their BSA-conjugates stimulate macrophage migration. J Leukocyte Biol 41:248–256

    Google Scholar 

  47. Takano R, Nose M, Kanno H, Nishihira T, Hiraizumi S, Kobata A, Kyogoku M (1990) Recognition of N-glycosidic carbohydrates on esophageal carcinoma cells by macrophage cell line THP-1. Am J Pathol 137:393–401

    Google Scholar 

  48. Tavassoli M, Hardy CL (1990) Molecular basis of homing of intravenously transplanted stem cells to the marrow. Blood 76:1059–1070

    Google Scholar 

  49. Zipori D, Sasson T, Frenkel A (1981) Myelopoiesis in the presence of stromal cells from mouse bone marrow. I. Monosaccharides regulate colony formation. Exp Hematol 9:656–663

    Google Scholar 

  50. Zuhrie SR, Pearson JD, Wickramasinghe SN (1988) Haemoglobin synthesis in K562 erythroleukemia cells is affected by intimate contact with monolayers of various human cell types. Leukemia Res 12:567–574

    Google Scholar 

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Tierärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstrasse 13, D-80539, Munich, Germany

    S. Gabius, R. Wawotzny, U. Martin, S. Wilholm & H. -J. Gabius

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  1. S. Gabius
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  2. R. Wawotzny
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  3. U. Martin
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  4. S. Wilholm
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  5. H. -J. Gabius
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Gabius, S., Wawotzny, R., Martin, U. et al. Carbohydrate-dependent binding of human myeloid leukemia cell lines to neoglycoenzymes, matrix-immobilized neoglycoproteins, and bone marrow stromal cell layers. Ann Hematol 68, 125–132 (1994). https://doi.org/10.1007/BF01727416

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  • DOI: https://doi.org/10.1007/BF01727416

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