Summary
We set up a laser nephelometric assay for the quantitation of serum amyloid A (SAA) in human plasma. Therefore monospecific antibodies were raised in sheep and used in parallel to measure SAA concentrations by nephelometry and also by radial immunodiffusion, an assay usually applied for determination of SAA. The nephelometric method is precise, simple and unlike radial immunodiffusion results are obtained within an hour. The antigen concentrations determined both by laser nephelometry and radial immunodiffusion correlated highly (r=0.98). As plasma SAA concentrations were reported to be a possible marker of kidney allograft rejection, the assay was applied to measure SAA concentrations in patients after kidney transplantation. Data were compared with clinical and other biochemical parameters. The period after kidney transplantation is reported for two cases, where SAA plasma concentrations were helpful in diagnosing allograft rejection. The rapid availability of the SAA plasma concentrations by nephelometry makes them a possible additional tool to decide quickly upon antirejection therapy.
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Abbreviations
- HDL:
-
high density lipoprotein
- IEF:
-
isoelectric focusing
- RID:
-
radial immunodiffusion
- SAA:
-
serum amyloid A
- SDS:
-
sodium dodecylsulfate
References
Benditt EP, Eriksen N (1977) Amyloid protein SAA is associated with high density lipoprotein from human serum. Proc Natl Acad Sci USA 74:4025–4028
Benson MD, Cohen AS (1979) Serum amyloid A protein in amyloidosis, rheumatic, and neoplastic diseases. Arthritis Rheum 22(1):36–42
Cham BE, Knowles BM (1976) A solvant system for deplipidation of plasma or serum without protein precipitation. J Lipid Res 7:176–181
Chambers RE, Whitcher JT (1983) Quantitative radial immunodiffusion for serum amyloid-A protein. J Immunol Methods 59:95–103
Godenir NL, Jeenah MS, Coetzee GA, van der Westhuysen DR, Strachnan AF, de Beer FC (1985) Standardization of the quantitation of serum amyloid A protein (SAA) in human serum. J Immunol Methods 83:217–225
Havel RJ, Eder HA, Bragdon J (1955) The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 34:1345–1353
Marcel YE, Bergsetz M, Nestruck AC (1979) Preparative isoelectric focusing of apolipoprotein C and E from human very low density lipoproteins. Biochim Biophys Acta 573:175–183
Maury CPJ, Teppo AM, Eklund B, Ahonen J (1983) Serum amyloid A protein: a sensitive indicator of renal allograft rejection in humans. Transplant 36:501–504
Maury CPJ, Höckerstedt K, Teppo AM, Lautenschlager I, Scheinin TM (1984) Changes in serum amyloid A protein and beta-2-Microglobulin in association with liver allograft rejection. Transplant 38:551–553
Maury CPJ, Teppo AM, Ahonen J von Willebrand E (1984a) Measurement of serum amyloid A protein concentrations as test of renal allograft rejection in patients with initially nonfunctioning grafts. Brit Med J 288:360–361
Maury CPJ (1985) Comparative study of serum amyloid A protein and C-reactive protein in disease. Clin Sci 68:233–238
Maury CPJ, Teppo AM, Ahonen J, von Willebrand E (1986) Evaluation of serum amyloid A protein as a marker of allograft rejection in patients with initially nonfunctioning renal transplants. Transplant Proc XVIII (1):86–87
Maury CPJ, Höckerstedt K, Lautenschlager I and Scheinin TM (1987) Monitoring of high density lipoprotein-associated amyloid A protein after liver transplantation. Transplant Proc XIX (5):3825–3826
Radola BJ (1974) Isoelectric focusing in layers of granulated gels II. Preparative isoelectric focusing. Biochim Biophys Acta 386:181–195
Raynes JG, Cooper EH (1983) Comparison of serum amyloid A protein and C-reactive protein concentrations in cancer and non-malignant disease. J Clin Pathol 36:798–803
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Parts of this work were reported at the 21st Annual Meeting of the European Society for Clinical Investigation, Copenhagen, March 1987
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Hocke, G., Ebel, H., Bittner, K. et al. A rapid laser immunonephelometric assay for serum amyloid A (SAA) and its application to the diagnosis of kidney allograft rejection. Klin Wochenschr 67, 447–451 (1989). https://doi.org/10.1007/BF01725141
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DOI: https://doi.org/10.1007/BF01725141