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Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains

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Summary

We have sequenced gene 5 encoding NSP1 for three human, two porcine, two bovine, one feline, and five equine rotavirus strains, and compared the nucleotide and deduced amino acid sequences with the published sequences for other various strains. Subgroup I human strains L26, 69M, and DS-1 were found to have a similar NSP1 sequence despite their different G serotypes, VP4 genotypes, and RNA patterns. The NSP1 sequence of the human strain K8 showed a high degree of homology to those of porcine strains OSU and YM. A high degree of homology was found among three equine strains (H2, FI-14, and FI23), but they differed from the other equine strains L338 and H1. The strain H1 was similar to the porcine strains. The feline strain Cat2 showed a high homology to bovine strains UK, RF, and A44. Thus, species-specific and interspecies relatedness of NSP1 sequences among human, porcine, bovine, feline and equine rotaviruses was found. Overall genomic relatedness of strains L26 and YM to various human and animal strains was also examined by RNA-RNA hybridization assay. The present and previous hybridization results showed that there is a good correlation in most strains between overall genomic property (or genogroup) and NSP1 sequence homology.

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Authors and Affiliations

  1. Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan

    K. Kojima, K. Taniguchi & N. Kobayashi

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  1. K. Kojima
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  2. K. Taniguchi
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  3. N. Kobayashi
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Additional information

Sequence data from this article have been deposited with the GSDB, DDBJ, EMBL and NCBI Data Libraries under Accession Nos. D38150 (strain L26), D38151 (strain 69M), D38152 (strain K8), D38153 (strain OSU), D38154 (strain YM), D38155 (strain FI-14), D38156 (strain FI23), D38157 (strain H2), D38158 (strain L338), U23726 (strain A44), D38148 (strain A5-13), U23727 (strain Cat2), and U23728 (strain H1).

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Kojima, K., Taniguchi, K. & Kobayashi, N. Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains. Archives of Virology 141, 1–12 (1996). https://doi.org/10.1007/BF01718584

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  • DOI: https://doi.org/10.1007/BF01718584

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