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Characterization of Toronto virus capsid protein expressed in baculovirus

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Summary

Toronto virus (TV), previously called “minireovirus,” a human calicivirus classified as genogroup 2 and phylogenetic type P2-A, was originally described in association with diarrhea in children. The second open reading frame, encoding the capsid protein of TV24, was expressed in a baculovirus recombinant. The recombinant baculovirus produced a protein (rTV) with an apparent molecular mass of 58 kDa that self-assembled into virus-like particles ∼ 30 nm in diameter with a density of 1.29 g/ml. Antigenic and immunogenic characteristics of these particles were determined by protein immunoblot, immunoprecipitation, and enzyme immunoassay. Seroconversion to the rTV protein was detected in 6 of 8 (75%) patients from a recent outbreak of gastroenteritis associated with a virus of similar phylogenetic type. These results confirm and extend the previous reports of the expression of the Norwalk and Mexico virus capsid proteins.

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Authors and Affiliations

  1. Viral Gastroenteritis Section, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

    J. P. G. Leite, T. Ando, J. S. Noel, B. Jiang, R. I. Glass & S. S. Monroe

  2. Department of Virology, Oswaldo Curz Institute, Rio de Janeiro, Brazil

    J. P. G. Leite

  3. Molecular Pathology and Ultrastructure Activity, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

    C. D. Humphrey

  4. Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

    J. F. Lew & K. Y. Green

Authors
  1. J. P. G. Leite
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  2. T. Ando
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  3. J. S. Noel
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  4. B. Jiang
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  5. C. D. Humphrey
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  6. J. F. Lew
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  7. K. Y. Green
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  8. R. I. Glass
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  9. S. S. Monroe
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Leite, J.P.G., Ando, T., Noel, J.S. et al. Characterization of Toronto virus capsid protein expressed in baculovirus. Archives of Virology 141, 865–875 (1996). https://doi.org/10.1007/BF01718161

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  • DOI: https://doi.org/10.1007/BF01718161

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