Abstract
Over many years, the baculovirus expression vector system (BEVS) has grown in popularity as a platform for expressing recombinant proteins. In addition to high levels of expression, the recombinant protein is near authentic as the insect cell is capable of posttranslational modifications such as glycosylation, oligomerization, disulfide bond formation, acylation, and phosphorylation.
The BEVS method has been particularly successfully employed to produce virus-like particles (VLP) from a variety of enveloped and non-enveloped viruses. VLPs are recombinant viral structural proteins which assemble spontaneously into a virus-like structure which is structurally similar to the authentic virus but devoid of any genomic material, providing a safe choice compared to the risk associated with the handling of live virus. Currently, many experimental vaccine prototypes have adopted the BEVS as their choice production VLP based platform as well as for the development of diagnostics for viral diseases. Following immunization of laboratory animals, insect cell generated VLPs have produced antigen-specific antibody consistent with a protective capacity equivalent to commercially available live or inactivated vaccines.
In this chapter we provide a detailed protocol for the purification of VLPs of an FMDV O serotype using the baculovirus expression vector system. The process illustrated by this method may be effectively used for the development of vaccine candidate and diagnostics against other burdensome viral diseases such as PCV2, PRRS, or Avian influenza.
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This review has been promoted and supported by Felix Trust and The Department of Biotechnology, Government of India.
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Maity, H.K., Deb, R., Lyons, S., Jones, I.M. (2022). Production of Virus-like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis. In: Deb, R., Yadav, A.K., Rajkhowa, S., Malik, Y.S. (eds) Protocols for the Diagnosis of Pig Viral Diseases. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2043-4_25
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DOI: https://doi.org/10.1007/978-1-0716-2043-4_25
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