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Cross-reactivity of monoclonal antibodies and sera directed against lipid A and lipopolysaccharides

Kreuzreaktion monoklonaler Antikörper und Antisera gegen Lipid A und Lipopolysaccharid (LPS)

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Summary

The cross-reactive capacity of monoclonal and polyclonal lipid A antibodies was tested with rough and smooth lipopolysaccharides (LPS). The antibodies represented different specificities recognizing epitopes in the hydrophilic lipid A backbone. In none of the various assay systems applied did the antibodies react with complete rough or smooth-form LPS. Cross-reactions, in general, were only detected with the most rudimentary rough LPS tested, i.e. Re-LPS. A variety of reactivities with other LPS was shown not to be related to lipid A antibodies; such reactivities were present in rabbit sera as well as in crude ascites. These results underline the need for careful checks on the origin of reactivities observed. In addition, rabbit antisera raised with R- and S-LPS were screened for lipid A reactvity using synthetic lipid A and partial structures as antigens. No cross-reactivity of LPS antibodies with lipid A was detected in these sera.

Zusammenfassung

Die Kreuzreaktion monoklonaler und polyklonaler Lipid A-Antikörper mit R- und S-Form LPS wurde getestet. Eingesetzt wurden Antikörper unterschiedlicher Spezifitäten, die Epitope im hydrophilen Bereich von Lipid A erkennen. In keinem der verschiedenen Testsysteme reagierten die Antikörper mit komplettem R- oder mit S-LPS. Kreuzreaktionen traten im allgemeinen nur mit der kleinsten Rauhform auf, dem Re-LPS. Eine Reihe von Reaktionen mit anderen LPS wurde nicht durch die Lipid A-Antikörper sondern durch andere Faktoren verursacht: derartige Reaktionen wurden sowohl mit Kaninchenseren als auch mit ungereinigtem Ascites beobachtet. Die Ergebnisse zeigen die Bedeutung sorgfältiger Überprüfung der Herkunft von beobachteten Reaktionen. Weiterhin wurden Kaninchenseren gegen R- und S-LPS mit synthetischen Lipid A-Antigenen auf Lipid A-Reaktivität untersucht; es wurde keine Kreuzreaktion der LPS- Antikörper mit Lipid A beobachtet.

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  1. c/o L. Brade, Div. of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Parkallee 22, W-2061, Borstel, Germany

    Hella-Monika Kuhn

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Kuhn, HM. Cross-reactivity of monoclonal antibodies and sera directed against lipid A and lipopolysaccharides. Infection 21, 179–186 (1993). https://doi.org/10.1007/BF01710544

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