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The microinjection of adenosine triphosphate into the protoplasm of skeletal muscle fibers

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Summary

  1. 1.

    The average shortening of frog muscle fiber produced by the microinjection of 0.001 M ATP, pH 7.0 as compared with a sodium ion control was about 13%.

  2. 2.

    The microinjection of 0.003 M ATP containing 0.5 M phosphate ion was insignificant in shortening muscle protoplasm at ranges of sodium ion from 0.1 to 0.4 M.

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Bibliography

  • Abdon, N. O., 1942: Dissert. Lund, cit. Buchthal and Falkow (1944).

  • Acierno, L., F. Burno, F. Burstein, and J. R. DiPalma, 1952: Actions of Adenosine Triphosphate on the Isolated Cat Atrium and their Antagonism by Acetyldioline. J. Pharmacol. (Am.)104, 264–268.

    Google Scholar 

  • Babsky, E. B., and P. F. Minajev, 1946: Combined action upon muscle of Adenosine Triphosphatase, Acetyldioline, and Potassium, Calcium and Magnesium ions. Nature158, 238.

    Google Scholar 

  • Beznak, A. B. L., 1950: ATP and ACH in the contraction of the striated frog muscle. XVIII. International Physiological Congress103.

  • Buchthal, F., A. Deutsch, and G. C. Knappeis, 1944: Release of Contraction and Changes in Birefringence Caused by Adenosine Triphosphate in Isolated Cross Striated Muscle Fibers. Acta physiol. scand.8, 271–287.

    Google Scholar 

  • —, and B. Falkow, 1944: Close Arterial Injection of Adenosine Triphosphate and Inorganic Triphosphate into Frog Muscle. Acta physiol. scand.8, 312–316.

    Google Scholar 

  • Chambers, R., and M. J. Kopac, 1950: Handbook of Microscopical Technique. C. E. McClung, editor. 3rd Edition, New York.

  • Chambers, R., M. J. Kopac, and P. Resnikoff, 1926: The action of the chlorides of Na, K, Ca and Mg on the protoplasm ofAmoeba proteus. J. gen. Physiol. (Am.)8, 369–402.

    Google Scholar 

  • Cohn, W. E., and C. E. Carter, 1950: The separation of adenosine polyphosphates by ion exchange and. paper chromatography. Amer. chem. Soc.72, 4273.

    Google Scholar 

  • Falk, A., and R. W. Gerard, 1954: Effect of microinjected salts and ATP on the membrane potential and mechanical response of muscle. J. cellul. a. comp. Physiol. (Am.)43, 393–403.

    Google Scholar 

  • Fiske, C. H., and Y. Subbarow, 1925: The colorimetric determination of phosphorus. J. biol. Chem. (Am.)66, 375–400.

    Google Scholar 

  • — —, 1927: Nature of “inorganic phosphate” in voluntary muscle. Science65, 401–403.

    Google Scholar 

  • Fleckenstein, A., J. Janke, R. E. Davies, and H. A. Krebs, 1954: Contraction of Muscle without fission of Adenosine Triphosphate or Creatine Phosphate. Nature174, 1081–1083.

    Google Scholar 

  • Heilbrunn, L. V., and F. J. Wiercinski, 1947: The action of various cations on muscle protoplasm. J. of cellul. a. comp. Physiol. (Am.)29, 15–32.

    Google Scholar 

  • - 1952: An Outline of General Physiology, 3rd Edition. Philadelphia.

  • Le Page, G. A., and V. A. Potter, 1949: Effect of Trace Impurities in ATP. J. biol. Chem. (Am.)179, 1229–1233.

    Google Scholar 

  • Mommaerts, W. F. H. M., 1954: Is Adenosine Triphosphate broken down during a single muscle twitch? Nature174, 1083–1084.

    Google Scholar 

  • Morell, S. A., J. H. Lipton, and A. Frieden, 1951: Stability of Disodium Adenosine Triphosphate. Science114, 333.

    Google Scholar 

  • Ranney, R. E., 1954: Dissociation and Contractile Reactions of Actomyosin with Uridine Triphosphate. Amer. J. Physiol.178, 517–520.

    Google Scholar 

  • Robb, J. S., M. Grübb and H. Braunfelds, 1955: Shortening of Actomyosin Threads not induced by Adenosine Triphosphate. Amer. J. Physiol.181, 39–42.

    Google Scholar 

  • Szent-Györgyi, A., 1947: Chemistry of Muscular Contraction. 1st Edition. New York.

  • - 1951: Chemistry of Muscular Contraction, 2nd Edition. New York.

  • - 1953: Chemical Physiology of Contraction, in Body and Heart Muscle. New York.

  • Szent-Györgyi, A. G., 1950: Comparison of actomyosin formation in different muscle preparations. Enzymologia14, 246–251.

    Google Scholar 

  • Treloar, A. E., 1935: An Outline of Biometric Analysis. Minneapolis, Burgess, 1935.

  • Wiercinski, F. J., and B. A. Cookson, 1951: Experiments on the Microinjection of Adenosine Triphosphate into Skeletal Muscle Fibers. Fed. Proc. Part 1,10, 146.

    Google Scholar 

  • —, 1953: Microinjection of solutions into the isolated muscle fiber of the frog. Fed. Proc.12, No. 1, 155.

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Hahnemann Medical College, Philadelphia, Pa.

    F. J. Wiercinski

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  1. F. J. Wiercinski
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The author is grateful to Dr. Abel Lajtha of the Department of Pharmacology, New York State Psychiatric Institute for an analysis of the ATP preparations.

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Wiercinski, F.J. The microinjection of adenosine triphosphate into the protoplasm of skeletal muscle fibers. Protoplasma 47, 298–304 (1956). https://doi.org/10.1007/BF01705389

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  • DOI: https://doi.org/10.1007/BF01705389

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