Abstract
Recombinant baculoviruses were generated harboring the entire coding region of the S segment cDNA of Hantavirus strain CG 18–20 that belongs to the Puumala serotype. The recombinant nucleocapsid protein was expressed in Sf9 cells and shown to be antigenically identical with the authentic viral nucleocapsid protein by means of immunoblot analysis. Acute-phase and convalescent sera from European HFRS patients recognized the recombinant nucleocapsid protein in Western blots and the recombinant Baculovirus in indirect immunofluorescence assays. Insect cells infected with the recombinant Baculoviruses proved to be a suitable noninfectious substitute for Hantavirus-infected Vero E6 cells as an antigen source for immunodiagnostic assays allowing the detection of antibodies in HFRS patients.
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Lee H.W. and Lee P.W., Kor J Med19 371–383, 1976.
Baek L.J., Yanagihara R., Gibbs C.J.j., Miyazaki M., and Gajdusek D.C., J Gen Virol69 3129–3132, 1989.
Lee P.W., Gibbs C.J., Gajdusek D., and Yanagihara R., J Clin Microbiol22 940–944, 1985.
Gligic A., Dimkovic N., Yiao S.-Y., Buckle G.J., Jovanovic D., Velimirovic D., Stojanovic R., Obradovic M., Diglisic G., Micic J., Asher D.M., LeDuc J.W., Yanagihara R., and Gajdusek D.C., J Infect Dis166 113–120, 1992.
Gligic A., Obradovic M., Stojanovic R., Vujosevic N., Ovcaric A., Frusic M., Gibbs C.J.j., Calisher C.H., and Gajdusek D.C., Am J Trop Med Hyg41 102–108, 1989.
Niklasson B. and Leduc J., Lancet1 1012–1013, 1984.
Zöller L., Scholz J., Stohwasser R., Giebel L.B., Sethi K.K., Bautz E.K.F., and Darai G., J Med Virol27 231–237, 1989.
Niklasson B., Tkachenko E., Ivanov A.P., van der Groen G., Wiger D., Andersen H.K., LeDuc J., Kjellson T., and Nyström K., Res Virol141 637–648, 1990.
Schmaljohn C.S., Hasty S.E., Dalrymple J.M., Leduc J.W., Lee H.W., von Bonsdorff C.H., Brummer-Korvenkontio M., Vaheri A., Tsai T.F., Regnery H.L., Goldgaber D., and Lee P.W., Science227 1041–1044, 1985.
Schmaljohn C.S., Jennings G.B., Hay J., and Dalrymple J.M., Virology155 633–643, 1986.
Stohwasser R., Giebel L.B., Zöller L., Bautz E.K.F., and Darai G., Virology174 79–86, 1990.
Arikawa J., Lapenotiere H.F., Iacono-Connors L., Wang M., and Schmaljohn C.S., Virology176 114–125, 1990.
Parrington M.A. and Kang C.Y., Virology175 167–175, 1990.
Zöller L., Yang S., Gött P., Bautz E.K.F., and Darai G., J Med Virol39 200–207, 1993.
Groen J., Dalrymple J., Fisher-Hoch S., Jordans H.G.M., Clement J.P., and Osterhaus A.D.M.E., J Med Virol.37 283–287, 1992.
Schmaljohn C.S., Sugiyama K., Schmaljohn A.L., and Bishop D.H., J Gen Virol69 777–786, 1988.
Schmaljohn C.S., Chu Y.K., Schmaljohn A.L., and Dalrymple J.M., J Virol64 3162–3170, 1990.
Summers M.D. and Smith G.E., Texas Agricultural Experiment Station Bulletin no. 1555, 1987.
Luckow V.A. and Summers M.D., Bio/Technology6 47–55, 1988.
Chen E.Y. and Seeburg P.H., DNA4 165–170, 1985.
Pen J., Welling G.W., and Welling-Wester S., Nucleic Acids Res17 451, 1989.
Laemmli U.K., Nature227 680–685, 1970.
Kyse-Andersen J., J Biochem Biophys Methods10 203–209, 1984.
Zöller L., Yang S., Gött P., Bautz E.K.F., and Darai G., J Clin Microbiol31 1194–1199, 1993.
Matsuura Y., Possee R.D., Overton H.A., and Bishop D.H.L., J Gen Virol68 1233–1250, 1987.
Antic D., Kang C.Y., Spik K., Schmaljohn C., Vapalahti O., and Vaheri A., Virus Res24 35–46, 1992.
Barber G.N., Clegg J.C.S., and Lloyd G., J Gen Virol71 19–28, 1990.
Brown C.S., van Bussel M.J., Wassenaar A.L.M., van Elsacker-Niele A.M.W., Weiland H.T., and Salismans M., J Virol Meth29 53–62, 1990.
Rota P.A., Black R.A., De B.K., Harmon M.W., and Kendal A.P., J Gen Virol71 1545–1554, 1990.
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Schuldt, C., Zöller, L., Bautz, E.K.F. et al. Baculovirus expression of the nucleocapsid protein of a Puumala serotype Hantavirus. Virus Genes 8, 143–149 (1994). https://doi.org/10.1007/BF01703071
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DOI: https://doi.org/10.1007/BF01703071