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Clonal heterogeneity, distribution, and pathogenicity of methicillin-resistantStaphylococcus aureus

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Abstract

Four thousand eighty-eightStaphylococcus aureus isolates obtained from patients hospitalised in a university clinic and four community hospitals over a period of one year were screened for methicillin resistance. A resistance rate of 5% was detected among initial isolates. Distribution of methicillin-resistantStaphylococcus aureus (MRSA) and methicillin-sensitiveStaphylococcus aureus showed an increased prevalence of MRSA in clinically significant specimens such as blood, central venous catheter tips, bronchial secretions, and wound secretions. Typing of 110 MRSA strains (initial isolates) by macrorestriction analysis of chromosomal DNA revealed 26 different genotypes that could be divided into five epidemic and 21 sporadic strains. More than 50% of all isolates belonged to one type that was confirmed to be closely related to the “southern-German” epidemic strain. Production of virulence factors such as enterotoxin A-D and toxic shock syndrome-toxin 1 among MRSA strains (initial isolates) occurred in ten of 26 different MRSA types. A strong correlation between genotype and toxin production was demonstrated.

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References

  1. Jevons MP: “Celbenin”-resistant staphylococci. British Medical Journal 1961, 1: 124–125.

    Google Scholar 

  2. Wenzel RP: The emergence of methicillin-resistantStaphylococcus aureus. Annals of Internal Medicine 1982, 97: 440–442.

    PubMed  Google Scholar 

  3. Aparicio P, Richardson J, Martin S, Vindel A, Marples RR, Cookson BD: An epidemic methicillin-resistant strain ofStaphylococcus aureus in Spain. Epidemiology and Infection 1992, 108: 287–298.

    PubMed  Google Scholar 

  4. Richardson JF, Reith S: Characterization of a strain of methicillin-resistantStaphylococcus aureus (EMRSA-15) by conventional and molecular methods. Journal of Hospital Infection 1993, 25: 45–52.

    PubMed  Google Scholar 

  5. Witte W, Cuny C, Braulke C, Hueck D: Clonal dissemination of two MRSA strains in Germany. Epidemiology and Infection 1994, 113: 67–73.

    PubMed  Google Scholar 

  6. Duckworth GJ: Diagnosis and management of methicillin-resistantStaphylococcus aureus infection. British Medical Journal 1993, 307: 1049–1052.

    PubMed  Google Scholar 

  7. Hartmann BJ, Tomasz A: Low-affinity penicillin-binding protein associated with β-lactam resistance inStaphylococcus aureus. Journal of Bacteriology 1984, 158: 513–516.

    PubMed  Google Scholar 

  8. Archer GL, Niemeyer DM: Origin and evolution of DNA associated with resistance to methicillin in staphylococci. Trends in Microbiology 1994, 2: 343–347.

    PubMed  Google Scholar 

  9. Lencastre H, Longe BLM, Matthews PR, Tomasz A: Molecular aspects of methicillin resistance inStaphylococcus aureus. Journal of Antimicrobial Chemotherapy 1994, 33: 7–24.

    PubMed  Google Scholar 

  10. Voss A, Milatovic D, Wallrauch-Schwarz C, Rosdahl VT, Braveny I: Methicillin-resistantStaphylococcus aureus in Europe. European Journal of Clinical Microbiology & Infectious Diseases 1994, 13: 50–55.

    Google Scholar 

  11. Panlilio AL, Culver DH, Gaynes RP, Banerjee S, Henderson TS, Toison JS, Martone WJ: Methicillin-resistantStaphylococcus aureus in U.S. hospitals, 1975–1991. Infection Control and Hospital Epidemiology 1992, 13: 582–586.

    PubMed  Google Scholar 

  12. Kimura A, Igarashi H, Ushioda H, Okuzuki K, Kobayashi H, Otsuka T: Epidemiological study ofStaphylococcus aureus isolated from Japanese national university and medical college hospitals. Kansenshogaku-Zasshi 1992, 66: 1543–1549.

    PubMed  Google Scholar 

  13. Jordens JZ, Duckworth GJ, Williams RJ: Production of “virulence factors” by “epidemic” methicillin-resistantStaphylococcus aureus in vitro. Journal of Medical Microbiology 1989, 30: 245–252.

    PubMed  Google Scholar 

  14. Schmitz FJ, Geisel R, Wagner S, Lenz W, Idel H, Hadding U, Heinz HP: Bildung von Enterotoxin und Toxic Shock-Syndrom-Toxin 1 bei Methicillin-resistenten und -sensiblenStaphylococcus aureus-Stämmen. Laboratoriumsmedizin 1996, 20: 342–350.

    Google Scholar 

  15. Coja JE, Browning L, Haines L, Birkbeck TH, Platt DJ: Comparison of enterotoxins and haemolysins produced by methicillin-resistant (MRSA) and sensitive (MSSA)Staphylococcus aureus. Journal of Medical Microbiology 1992, 36: 164–171.

    PubMed  Google Scholar 

  16. Duckworth GJ, Oppenheim BA: Enterotoxin production in epidemic methicillin-resistantStaphylococcus aureus. Lancet 1986, i: 565–566.

    Google Scholar 

  17. Kaffka A: Erfahrungen mit einfachen Untersuchungsmethoden zum Nachweis von Hyaluronidase, Fibrinolysin und Phosphatase bei Staphylokokken. Zentralblatt für Bakteriologie 1957, 168: 381–385.

    Google Scholar 

  18. Schmalreck AF, Friedrich W, Kottmann I, Reiser A, Ruffer U, Schlenk R, Tränkle P, Vanca E, Wildfeuer A: Resistenzmuster von Staphylokokken und Nachweis des mecA-Gens. Chemotherapie Journal 1996, 5: 195–202.

    Google Scholar 

  19. Schneider C, Weindel M, Brade V: Frequency, clonal heterogeneity, and antibiotic resistance of methicillin-resistantStaphylococcus aureus (MRSA) isolated in 1992–1994. Zentralblatt für Bakteriologie 1996, 283: 529–542.

    Google Scholar 

  20. Schwarzkopf A, Cuny C, Witte W: Bestimmung der Fragmentmuster der genomischen DNA mittels Pulsfeld-Gelelektrophorese beiStaphylococcus aureus. Bundesgesundheitsblatt 1995, 6: 215–219.

    Google Scholar 

  21. Claus H, Cuny C, Pasemann B, Witte W: Ein Datenbank-system für Fragmentmuster der genomischen DNS vonStaphylococcus aureus. Bundesgesundheitsblatt 1996, 2: 68–74.

    Google Scholar 

  22. Voss A, Machka K, Lenz W, Milatovic D: Vorkommen, Häufigkeit und Resistenzverhalten von Methicillin-Oxacillin-resistentenStaphylococcus aureus-Stämmen in Deutschland. Deutsche Medizinische Wochenschrift 1992, 117: 1907–1912.

    PubMed  Google Scholar 

  23. Keane CT, Coleman CD, Cafferkey MT: Methicillin-resistantStaphylococcus aureus — a reappraisal. Journal of Hospital Infection 1991, 19: 147–152.

    PubMed  Google Scholar 

  24. Humphreys H: Comparison of infections caused by methicillin-sensitive and methicillin-resistantStaphylococcus aureus. In: Cafferkey MT (ed.): Methicillin-resistantStaphylococcus aureus. Marcel Dekker, New York, 1992, p. 77–90.

    Google Scholar 

  25. French GL, Cheng AFB, Ling JML, Mo P, Donnan S: Hong Kong strains of methicillin-resistantStaphylococcus aureus have similar virulence. Journal of Hospital Infection 1990, 15: 117–125.

    PubMed  Google Scholar 

  26. Lewis E, Saravolatz D: Comparison of methicillin-resistant and methicillin-sensitiveStaphylococcus aureus bacteremia. American Journal of Infection Control 1985, 13: 109–114.

    PubMed  Google Scholar 

  27. Coleman DC: Methicillin-resistantStaphylococcus aureus: molecular epidemiology and expression of virulence determinants. In: Cafferkey MT (ed.): Methicillin-resistantStaphylococcus aureus. Marcel Dekker, New York, 1992, p. 37–56.

    Google Scholar 

  28. Mizobuchi S, Minami J, Jin F, Matsushita O, Okabe A: Comparison of the virulence of methicillin-resistant and methicillin-sensitiveStaphylococcus aureus. Medical Microbiology and Immunology 1994, 38: 599–605.

    Google Scholar 

  29. Romero-Vivas J, Rubio M, Fernandez C, Picazo JJ: Mortality associated with nosocomial bacteremia due to methicillin-resistantStaphylococcus aureus. Clinical Infectious Diseases 1995, 21: 1417–1423.

    PubMed  Google Scholar 

  30. Pujol M, Pena C, Pallares R, Ariza J, Ayats J, Dominguez MA, Gudiol F: NosocomialStaphylococcus aureus bacteremia among nasal carriers of methicillin-resistant and methicillin-susceptible strains. American Journal of Medicine 1996, 100: 509–516.

    PubMed  Google Scholar 

  31. Lehn N, Schaller E, Wagner H, Kranke M: Frequency of toxic shock syndrome toxin and enterotoxin producing clinical isolates ofStaphylococcus aureus. European Journal of Clinical Microbiology & Infectious Diseases 1995, 14: 43–46.

    Google Scholar 

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Authors and Affiliations

  1. Department of Medical Microbiology, University Hospital of Frankfurt, Paul-Ehrlich-Str. 40, 60596, Frankfurt am Main, Germany

    T. A. Wichelhaus, J. Schulze, K. P. Hunfeld, V. Schäfer & V. Brade

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  1. T. A. Wichelhaus
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  2. J. Schulze
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  3. K. P. Hunfeld
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  4. V. Schäfer
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  5. V. Brade
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Wichelhaus, T.A., Schulze, J., Hunfeld, K.P. et al. Clonal heterogeneity, distribution, and pathogenicity of methicillin-resistantStaphylococcus aureus . Eur. J. Clin. Microbiol. Infect. Dis. 16, 893–897 (1997). https://doi.org/10.1007/BF01700555

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