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Genetically engineered resistance to potato virus X in four commercial potato cultivars

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Abstract

The genes for the capsid protein (CP) and the 8K movement protein of PVX were introduced into potato (Solanum tuberosum L.) and expressed under the control of CaMV 35S promoter using a binary vector andAgrobacterium tumefaciens. Four commercial potato cultivars (Russet Burbank, Shepody, Desirée and Bintje) have been efficiently transformed. Eleven independent transgenic clones, with CP expression levels higher than 0.05% of the soluble leaf proteins, were analyzed for resistance to inoculation with PVX (5 and 50µg/ml). The resistance of the transgenic plants to PVX was observed with the lower titer of virus inoculation (5 µg/ml) but not with higher titer (50 µg/ml). A significant reduction in the accumulation of virus in the inoculated transgenic potato plants has been observed under greenhouse and field conditions. Furthermore, the CP gene is very stable and is transferred to new plants originated from stem cuttings or from tubers. The transgenic plants appeared to be phenotypically identical to the nontransformed controls.

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Abbreviations

BAP:

benzyl-aminopurine

BCIP:

5-bromo-4-chloro-3-indolylphosphate p-Toluidine salt

CaMV:

cauliflower mosaic virus

CP:

capsid protein

GA3 :

gibberellic acid

Kbp:

kilobase pair

NAA:

naphthalene acetic acid

NBT:

nitroblue tetrazolium chloride

NOS:

nopaline synthase

NPT II:

neomycin phosphotransferase II

PMSF:

phenyl methyl sulfonyl fluoride

PVX:

potato virus X

PVY:

potato virus Y

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Authors and Affiliations

  1. Department of Botany, University of Toronto, 25 Willcocks St., M5S 3B2, Toronto, Ontario, Canada

    Huimin Xu, Houri Khalilian, Mohamed Eweida & Mounir G. Abouhaidar

  2. Ontario Ministry of Agriculture, Food and Rural Affairs, 449 Dunlop St., L4N 1C3, Barrie, Ontario, Canada

    Sam Squire

Authors
  1. Huimin Xu
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  2. Houri Khalilian
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  3. Mohamed Eweida
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  4. Sam Squire
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  5. Mounir G. Abouhaidar
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Communicated by W. A. Parrott

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Xu, H., Khalilian, H., Eweida, M. et al. Genetically engineered resistance to potato virus X in four commercial potato cultivars. Plant Cell Reports 15, 91–96 (1995). https://doi.org/10.1007/BF01690261

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  • DOI: https://doi.org/10.1007/BF01690261

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