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Binding of trichodermin to mammalian ribosomes and its inhibition by other 12,13-epoxytrichothecenes

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Summary

Trichodermin binds to ribosomes from rabbit reticulocyte polysomes with an apparent Ka of 9.2 × 105 and with a maximum binding ratio of 0.44 molecules per ribosome. Trichodermin also binds firmly to HeLa cell polysomes under conditions where it is inhibiting protein synthesis. Purified HeLa 60S ribosomal subunits showed only a slight ability to bind trichodermin, and no binding was found with 40S subunits. Seventeen 12,13-epoxytrichothecenes were tested for their ability to interfere with [3H] trichodermin binding to reticulocyte ribosomes. All compounds known to inhibit ribosomal functions required for elongation-termination or initiation processes exhibited significant positive interference with the binding of trichodermin.

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References

  1. J. R. Bamburg and F. M. Strong, Microbial Toxins, S. Kadis, A. Cirgler, and S. J. Ajl, (Academic Press, (New York), Vol. VII, p. 207–292 (1971).

    Google Scholar 

  2. M. E. Stafford and C. S. McLaughlin, J. Cell Physiol. 82, 121–128 (1973).

    PubMed  Google Scholar 

  3. C. Wei and C. S. McLaughlin, Fed. Proc. 32, 494 Abs. (1973).

    Google Scholar 

  4. C. Wei, B. S. Hansen, M. H. Vaughan and C. S. McLaughlin, Proc. Natl. Acad. Sci. USA, in press. (1974).

  5. B. S. Hansen and M. H. Vaughan, Fed. Proc. 32, 494 Abs. (1973).

    Google Scholar 

  6. L. Carrasco, M. Barbacid and D. Vazquez, Biochim. Biophys. Acta 312, 368–376 (1973).

    PubMed  Google Scholar 

  7. W. P. Tate and C. T. Caskey, J. Biol. Chem. 248, 7970–7972 (1973).

    PubMed  Google Scholar 

  8. K. Ohtsubo, P. Kaden and C. Mittermayer, Biochim. Biophys. Acta 287, 520–525 (1972).

    PubMed  Google Scholar 

  9. E. Cundliffe, M. Cannon and J. Davies, Proc. Natl. Acad. Sci. USA, in press (1974).

  10. C. Wei and C. S. McLaughlin, Biochem. Biophys. Res. Commun., in press (1974).

  11. W. O. Godtfredson and S. Vangedal, Acta Chem. Scand. 19, 1088–1102 (1965).

    PubMed  Google Scholar 

  12. M. H. Schreir and T. Staehelin, J. Mol. Biol. 73, 329–349 (1973).

    PubMed  Google Scholar 

  13. M. H. Vaughan, J. R. Warner and J. E. Darnell, J. Mol. Biol. 25, 235–251 (1967).

    PubMed  Google Scholar 

  14. M. Reichman and S. Penman, Proc. Natl. Acad. Sci. USA 70, 2678–2682 (1973).

    PubMed  Google Scholar 

  15. H. Paulus, Anal. Biochem. 32, 91–100 (1969).

    PubMed  Google Scholar 

  16. M. Girard, H. Latham, S. Penman and J. E. Darnell, J. Mol. Biol. 11, 187–201 (1965).

    Google Scholar 

  17. D. H. Lockwood, U. Maitra, N. Brot and H. Weissbach, J. Biol. Chem., in press (1974).

  18. N. Brot, W. P. Tate, C. T. Caskey and H. Weissbach, Proc. Natl. Acad. Sci. USA, in press (1974).

  19. G. Sander, R. C. Marsh and A. Parmeggiani, Biochem. Biophys. Res. Commun. 47, 866–873 (1972).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Dept. of Molecular Biology and Biochemistry, Univ. of California, Irvine, 92664, Irvine, California, USA

    Cha-Mer Wei & Calvin S. McLaughlin

  2. Dept. of Biochem., Faculty of Arts and Sciences, Univ. of Pittsburgh, 15261, Pittsburgh, Pennsylvania, USA

    Iain M. Campbell & Maurice H. Vaughan

Authors
  1. Cha-Mer Wei
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  2. Iain M. Campbell
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  3. Calvin S. McLaughlin
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  4. Maurice H. Vaughan
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Wei, CM., Campbell, I.M., McLaughlin, C.S. et al. Binding of trichodermin to mammalian ribosomes and its inhibition by other 12,13-epoxytrichothecenes. Mol Cell Biochem 3, 215–219 (1974). https://doi.org/10.1007/BF01686646

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  • DOI: https://doi.org/10.1007/BF01686646

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