Summary
Immunocytochemical and histochemical properties of macrophages present in the subcutaneous chronic inflammatory responses surrounding adultOnchocerca volvulus (nodules) in human tissues were examined. Macrophages with strong non-specific esterase (NSE) and acid phosphatase (AcPase) activities but weak adenosine triphosphatase (ATPase) activity and HLA-DR expression (NSE+++, AcPase+++, ATPase−/+, HLA-DR−/+) were present in the centre of nodules. Many of the cells adhering to the surface of worms were NSE+++, AcPase+++, ATPase−, HLA-DR+++. The inner zone of the fibrous capsule of nodules contained macrophages with the profile NSE+++, AcPase−, ATPase−/+, HLA-DR−/+. A fourth type, NSE+++, AcPase−/+, ATPase−/+, HLA-DR+++, was located in the outer zone of the capsule, frequently within perivascular accumulations of macrophages, lymphocytes and plasma cells. Active fibroblasts were identified at the inner edge of the fibrous capsule by alkaline phosphatase staining. A feature of all nodules examined was the presence of lipid-filled macrophages, demonstrated by Oil Red O stain; these cells were usually situated in zones adjacent to the centre of nodules, and were of the NSE++, AcPase++, ATPase−/+, HLA-DR−/+ type. Lipid accumulation was not found to be related to the clinical status of the patients studied. The origin and functional significance of this lipid is unknown.
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References
ANDERSON, J. & FUGLSANG, H. (1977) Ocular onchocerciasis.Trop. Dis. Bull. 74 252–72.
BANCROFT, J. D. (1982) Enzyme histochemistry. InTheory and Practice of Histological Techniques (edited by BANCROFT, J. D. & STEVENS, A.), 2nd edn. Edinburgh, London, New York: Churchill Livingstone.
BAYLISS-HIGH, O. B. (1982) Lipids. InTheory and Practice of Histological Techniques (edited by BANCROFT, J. D. & STEVENS, A.), 2nd edn. Edinburgh, London, New York: Churchill Livingstone.
BYRAM, J. E. & LICHTENBERG, F. VON (1980) Experimental infection withSchistosoma mekongi in laboratory animals: parasitological and pathological findings.Malacological Review (edited by BRUCE, J. I., SORNMAN, S., ASCH, H. L. & CRAWFORD, K. A.), Suppl. 2, pp. 125–59. Michigan.
COMLEY, J. C. W. & JAFFE, J. J. (1981) Isoprenoid biosynthesis in adultBrugia pahangi andDirofilaria immitis.J. Parasitol. 67 609–16.
DRURY, R. & WALLINGTON, E. (1980) Lipids. InCarleton's Histological Technique, 5th edn. Oxford University Press.
ELLEDER, M., HRODEK, J. & CIHULA, J. (1983) Neimann-Pick disease: Lipid storage in bone marrow macrophages.Histochem. J. 15 1065–77.
GHALIB, H. W., MACKENZIE, C. D., WILLIAMS, J. F., EL SHEIK, H., EL KHALIFA, M. Y. & KRON, M. A. (1987) Severe onchocercal dermatitis in the Ethiopian border region of Sudan.Ann. Trop. Med. Parasitol. (in press).
GIBSON, D. W., HEGGIE, C. & CONNOR, D. H. (1980) Clinical and pathological aspects of onchocerciasis.Path. An. 15 195–240.
GUDERIAN, R. H., MOLEA, J., SWANSON, D., PROANO, S., CARRILLO, D. & SWANSON, W. L. (1983) Onchocerciasis in Ecuador. 1. Prevalence and distribution in the province of Esmeraldas.Tropenmed. Parasitol. 34 143–8.
HAQUE, A. & CAPRON, A. (1982) Transplacental transfer of rodent microfilariae induces antigen-specific tolerance in rats.Nature 299 361.
JANOSSY, G., BOFILL, M. & POULTER, L. W. (1986) Twocolour immunofluorescence: analysis of the lymphoid system with monoclonal antibodies. InImmunocytochemistry, 2nd edn (edited by POLAK, J. M. & VAN NOORDEN, S.). Bristol: Wright.
KWA, B. H. & MAK, J. W. (1980) Specific depression of cell mediated immunity in Malayan filariasis.Trans. R. Soc. Trop. Med. Hyg. 74 522–7.
MACKENZIE, C. D. (1980) Eosinophil leucocytes in filarial infections.Trans. R. Soc. Trop. Med. Hyg. 74 (Suppl.) 51–8.
MACKENZIE, C. D., JUNGERY, M., TAYLOR, P. M. & OGILVIE, B. M. (1981) Thein vitro interaction of eosinophils, neutrophils, macrophages and mast cells with nematode surfaces in the presence of complement or antibodies.J. Pathol. 133 161–75.
MACKENZIE, C. D., OXENHAM, S. L., GATRILL, A., ANDREW, S., GRENNAN, D. & DENHAM, D. A. (1985a) Mononuclear and multinuclear macrophages in filarial infections.Immunol. Lett. 11 239–46.
MACKENZIE, C. D., WILLIAMS, J. F. & GUDERIAN, R. H. (1986) Clinical responses in human onchocerciasis: parasitological and immunological implications. Ciba Foundation Symposium No. 127. Singapore, April 1986.Filariasis, 46–67.
MACKENZIE, C. D., WILLIAMS, J. F. & GUDERIAN, R. H. (1987) The onchocercal nodule: Its structural and cellular composition. InChemotherapy of Onchocerciasis (edited by CONDER, G. C. Kalamazoo: Upjohn (in press).
MACKENZIE, C. D., WILLIAMS, J. F., SISLEY, B. M., STEWARD, M. W. & O'DAY, J. (1985b) Variations in host immune responses in the pathogenesis of human onchocerciasis.Rev. Inf. Dis. 7 802–8.
MASON, D. Y., ABDULAZIZ, Z., FALINI, B. & STEIN, H. (1983) Double immunoenzymatic labeling. InImmunocytochemistry (edited by POLAK, J. M. & VAN NOORDEN, S.) Bristol: Wright.
MCMAHON, J. E., DAVIES, J. B., WHITE, M. D., GODDARD, J. M., BEECH-GARWOOD, P. A. & KIRKWOOD, B. R. (1986) Onchocerciasis in Sierra Leone 1. Studies on the prevalence and transmission at Gbaima village.Trans. R. Soc. Trop. Med. Hyg. 80 802–9.
PARKHOUSE, R. M. E., BOFILL, M., GOMEZ PRIEGO, A. & JANOSSY, G. (1985) Human macrophages and T-lymphyocyte subsets infiltrating nodules ofOnchocerca volvulus.Clin. Exp. Immunol. 62 13–18.
PIESSENS, W. F., PARTONO, F., HOFFMAN, S. L., RATIWAYANTO, S., PIESSENS, P. W., PALMIERI, J. R., KOIMAN, I., DENNIS, D. T. & CARNEY, W. P. (1982) Antigen-specific suppressor T-lymphocytes in human lymphatic filariasisNew Eng.J. Med. 307 144–8.
POULTER, L. (1983) Antigen presenting cellsin situ: their identification and involvement in immunopathology.Clin. Exp. Immunol. 53 513–20.
SELBY, W. S., POULTER, L. W., HOBBS, S., JEWELL, D. P. & JANOSSY, G. (1983) Heterogeneity of HLA-DR-positive histiocytes in human intestinal lamina propria: a combined histochemical and immunohistological analysis.J. Clin. Pathol. 36 379–84.
STEVENS, A. (1982) Pigments and minerals. InTheory and Practice of Histological Techniques (edited by BANCROFT, J. D. & STEVENS, A.), 2nd edn. Edinburgh, London, New York: Churchill Livingstone.
WILLIAMS, J. F., GHALIB, H. W., MACKENZIE, C. D., EL KHALIFA, M. Y., AYUYA, J. M. & KRON, M. A. (1987) Cell adherence to microfilariae ofOnchocerca volvulus: a comparative study. Ciba Foundation Symposium No. 127, Singapore, 1986.Filariasis, 46–67.
YIN FOO, D., NOWAK, M., COPEMAN, B. & MCCABE, M. (1983) A low molecular weight immunosuppressive factor produced byOnchocerca gibsoni.Vet. Immunol. Immunopathol. 4 445–51.
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Gatrill, A.J., Mackenzie, C.D., McMahon, J.E. et al. A histocytochemical study of the macrophages present in tissue responses to adultOnchocerca volvulus . Histochem J 19, 509–519 (1987). https://doi.org/10.1007/BF01675422
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DOI: https://doi.org/10.1007/BF01675422