Summary
Pineal protein synthesis in female rats, estimated from the incorporation of labeled amino acids into proteinsin vitro, exhibited significant changes as a function of the stage of the estrous cycle. These changes were restricted to the proestrous and estrous days; pineal protein synthesis attained its maximum on the morning of proestrus declining abruptly by 53% during the evening, at the time of the expected gonadotrophin and prolactin release. Pineal serotonin-N-acetyltransferase activity increased by 10 to 15 times during night-time on every day of cycle; no appreciable modification of its daily rhythm was detected along the estrous cycle. Spayed rats treated for 2 days with progesterone showed a dose-dependent decrease of amino acid incorporation into pineal proteins, regardless of whether estradiol was simultaneously administered or not. Pineal protein synthesis in spayed rats administered with estradiol for 2 days and killed at 11 a.m. and 5 p.m. on the third day, did not show differences as a function of time of sacrifice. When progesterone was injected on the morning of the third day a significant decline in protein synthesis was observed at 5 p.m. Only in the latter group serum LH levels showed significantly greater values at 5 p.m. Pineal serotonin content of estradiol-treated rats increased significantly at evening, an effect which was obliterated by the administration of progesterone; progesterone alone did not affect pineal serotonin content. Radioactivity uptake by pineal glands incubated with labeled progesterone did not show changes along the estrous cycle. These data argue in favour of the involvement of progesterone in the changes of pineal protein synthesis observed during the “critical period” for gonadotrophin and prolactin release.
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References
Axelrod, J. The pineal gland: a neurochemical transducer. Science184, 1341–1348 (1974).
Baulieu, E.-E., Atger, M., Best-Belpomme, M., Corvol, P., Courvalin, J.-C., Mester, J., Milgrom, E., Robel, P., Rochefort, H., De Catalogne, D. Steroid hormonereceptors. Vitam. Horm.33, 649–736 (1975).
Cardinali, D. P. Melatonin and the endocrine role of the pineal organ. In: Current Topics in Experimental Endocrinology, Vol. 2 (James, V. H. T., Martini, L., eds.), pp. 107–128. New York: Academic Press. 1974.
Cardinali, D. P. Nuclear receptor estrogen complex in the pineal gland. Modulation by sympathetic nerves. Neuroendocrinology24, 333–346 (1977).
Cardinali, D. P., Gomez, E., Rosner, J. M. Changes in3H-leucine incorporation into pineal proteins following estradiol or testosterone administration: Involvement of the sympathetic superior cervical ganglion. Endocrinology98, 849–858 (1976 a).
Cardinali, D. P., Nagle, C. A., Rosner, J. M. Metabolic fate of androgens in the pineal organ. Uptake, binding to cytoplasmic proteins and conversion of testosterone into 5α-reduced metabolites. Endocrinology95, 179–187 (1974 a).
Cardinali, D. P., Nagle, C. A., Rosner, J. M. Changes in the pineal indole metabolism and plasma progesterone levels during the estrous cycle in ewes. Steroids Lipids Res.5, 308–315 (1974 b).
Cardinali, D. P., Nagle, C. A., Rosner, J. M. Gonadal steroids as modulators of the function of the pineal gland. Gen. Comp. Endocr.26, 50–58 (1975 a).
Cardinali, D. P., Nagle, C. A., Rosner, J. M. Control of estrogen and androgen receptors in the rat pineal gland by catecholamine transmitter. Life Sci.16, 93–106 (1975 b).
Cardinali, D. P., Nagle, C. A., Rosner, J. M. Gonadotrophin- and prolactin-induced increase of rat pineal hydroxyindole-O-methyltransferase. Involvement of the sympathetic nervous system. J. Endocrinol.68, 341–342 (1976 b).
David, G. F. X., Umberkoman, B., Kumar, K., Anand Kumar, T. C. Neuroendocrine significance of the pineal. In: Brain-Endocrine interaction II. Second Int. Symp. Shizuoka 1974 (Knigge, K., Scott, D., Kobashi, H., Ishii, S., eds.), pp. 365–375. Basel: Karger. 1975.
Deguchi, T., Axelrod, J. Sensitive assay for serotonin-N-acetyltransferase activity in rat pineal. Anal. Biochem.50, 174–179 (1972).
Gorski, J., Gannon, F. Current models of steroid hormone action: a critique. Ann. Rev. Physiol.38, 425–450 (1976).
Hanukoglu, I., Karavolas, H. J., Goy, R. W. Progesterone metabolism in the pineal, brain stem, thalamus, and corpus callosum of the female rat. Brain Res.125, 313–324 (1977).
Houssay, A. B., Barcelo, A. C. Effect of estrogens and progesterone upon the biosynthesis of melatonin by the pineal gland. Experientia28, 478–479 (1972).
Hyyppä, M. T., Cardinali, D. P., Baumgarten, H. G., Wurtman, R. J. Rapid accumulation of3H-serotonin in brains of rats receiving3H-tryptophan: Effects of 5, 6-dihydroxytryptamine or female sex hormones. J. Neural Transmission34, 111–124 (1973).
Illnerova, H. Effect of estradiol on the activity of serotonin N-acetyltransferase in the rat epiphysis. Endocrinol. Exp. (Bratisl.)9, 141–148 (1975).
Klein, D. C., Weller, J. L. Indole metabolism in the pineal gland: A circadian rhythm in N-acetyltransferase. Science169, 1093–1095 (1970).
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J. Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
Luttge, W. G., Wallis, C. G. In vitro accumulation and saturation of3H-progestins in selected brain regions and in the adenohypophysis, uterus and pineal of the female rat. Steroids22, 493–502 (1973).
Lynch, H. J., Ho, M., Wurtman, R. J. The adrenal medulla may mediate the increase in pineal melatonin synthesis induced by stress but not that caused by exposure to darkness. J. Neural Transmission40, 87–98 (1977).
Marks, B. H., Wu, T. K., Goldman, H. Soluble estrogen binding protein in the rat pineal gland. Res. Comm. Chem. Path. Pharmac.3, 595–599 (1972).
Morin, L. P., Fitzgerald, K. M., Zucker, I. Estradiol shortens the period of hamster circadian rhythms. Science196, 305–307 (1977).
Nagle, C. A., Neuspiller, N., Cardinali, D. P., Rosner, J. M. Uptake and effects of 17β-estradiol on pineal hydroxyindole-O-methyl-transferase (HIOMT) activity. Life Sci.11 (II), 1109–1116 (1972).
Neill, J. D., Smith, M. S. Pituitary-ovarian interrelationships in the rat. In: Currect Topics in Experimental Endocrinology (James, V. H. T., Martini, L., eds.), pp. 73–106. New York: Academic Press. 1974.
Niswender, G. D., Midgley, A. R., Jr., Monroe, S. E., Reichert, L. D., Jr. Radioimmunoassay for rat luteinizing hormone with antiovine LH serum and ovine LH131I. Proc. Soc. Exp. Biol. Med.128, 807–811 (1968).
Pavel, S., Dumitru, I., Klepsch, I. A gonadotrophin inhibiting principle in the pineal of human fetuses. Evidence for its identity with arginine vasotocin. Neuroendocrinology13, 41–46 (1973–1974).
Preslock, J. P. Regulation of pineal enzymes by photoperiod, gonadal hormones and melatonin in Coturnix quail. Horm. Res.7, 108–117 (1976).
Quay, W. B. Circadian rhythm in rat pineal serotonin and its modifications by estrous cycle and photoperiod. Gen. comp. Endocr.3, 473–479 (1963).
Relkin, R. The Pineal. Montreal: Eden Press. 1976.
Stumpf, W. E., Sar, M., Keefer, D. A., Martinez-Vargas, M. C. The anatomical substrate of neuroendocrine regulation as defined by autoradiography with3H-estradiol,3H-testosterone,3H-dihydrotestosterone and3H-progesterone. In: Neuroendocrine Regulation of Fertility (Anand Kumar, T. C., ed.), pp. 46–56. Basel: Karger. 1976.
Trentini, G. P., Gaetani, C. F., Barbieri Palmieri, F. The role of sympathetic innervation in pineal-pituitary feedback. Blocking of pineal changes induced in the rat by experimental hypergonadotrophinaemia by chronic treatment with bretylium or guanethidine. Ann. Endocrinol.34, 261–270 (1973).
Wallen, E. P., Yochim, J. M. Rhythmic function of pineal hydroxyindole-O-methyltransferase during the estrous cycle: an analysis. Biol. Reprod.10, 461–466 (1974).
Wurtman, R. J., Axelrod, J., Snyder, S. H. Changes in the enzymatic synthesis of melatonin in the pineal during the estrous cycle. Endocrinology76, 798–800 (1965).
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Cardinali, D.P., Irene Vacas, M. Progesterone-induced decrease of pineal protein synthesis in rats. Possible participation in estrous-related changes of Pineal function. J. Neural Transmission 42, 193–205 (1978). https://doi.org/10.1007/BF01675310
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DOI: https://doi.org/10.1007/BF01675310