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Distribution of (Na++K+)ATPase and sodium channels in skeletal muscle and electroplax

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Journal of Neurocytology

Summary

The distributions of (Na+ + K+)ATPase and sodium channels in skeletal muscle fibres and electrocytes were determined by immunofluorescent and immunoelectron microscopic techniques using antibodies against rat and eel (Na+ + K+)ATPase and the eel electric organ sodium channel. The extrajunctional sarcolemma of skeletal muscle was uniformly stained by polyclonal antibodies against (Na+ + K+)ATPase and the sodium channel. The T-tubule system of skeletal muscle was also labelled heavily for both (Na+ + K+)ATPase and the sodium channel. The terminal cisternae of the sarcoplasmic reticulum was stained for (Na+ + K+)ATPase but not sodium channels. At the motor endplate, (Na+ + K+)ATPase-like immunoreactivity was present along the plasmalemma of motor nerve terminals but not along the postsynaptic junctional sarcolemma. Paradoxically, a monoclonal antibody that binds to the α form of the catalytic subunit of (Na+ + K+)ATPase from rat hepatocytes and renal tubule cells did not label the enzyme in rat skeletal muscle. In electrocytes, (Na+ + K+)ATPase-like irnmunoreactivity was concentrated primarily along the plasmalemma and calveolae of the non-innervated face. In contrast, sodium channel-like immunoreactivity was concentrated along the plasmalemma of the innervated face except in the clefts of the postsynaptic membrane. Thus, we conclude that at endplates both the (Na+ + K+)ATPase of rat skeletal muscle and sodium channels of eel electrocytes are not concentrated in the juxtaneuronal postsynaptic membrane. We also interpret the failure of the monoclonal anti-α (Na+ + K+)ATPase antibodies to bind to the enzyme in muscle to indicate that the catalytic subunit of skeletal muscle (Na+ + K+)ATPase displays different epitopes than does the a subunit of kidney and liver.

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Authors and Affiliations

  1. Laboratory for Neurocytology, Department of Neurosciences, School of Medicine, University of California, 92093, San Diego, La Jolla, California, USA

    R. G. Ariyasu, T. J. Deerinck & M. H. Ellisman

  2. Department of Physiology, University of Colorado Health Services Center, 80262, Denver, Colorado, USA

    S. R. Levinson

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  1. R. G. Ariyasu
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  2. T. J. Deerinck
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  3. S. R. Levinson
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  4. M. H. Ellisman
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Ariyasu, R.G., Deerinck, T.J., Levinson, S.R. et al. Distribution of (Na++K+)ATPase and sodium channels in skeletal muscle and electroplax. J Neurocytol 16, 511–522 (1987). https://doi.org/10.1007/BF01668505

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  • DOI: https://doi.org/10.1007/BF01668505

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