Summary
A Ficoll-gradient method to recover separately lymphocytes and sedimented cells from uterotubal flush fluid of cattle is described. Lymphocytes could be cocultured for virus isolation or used for histopathological studies, or both. Epithelioid cells, most likely of oviductal origin, were grown in monolayer cultures to initiate cell lines and isolate viruses. This technique could be applied to other situations that require the growth of bovine oviduct epithelial cells in vitro.
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Bouillant, A. M. P.; Ruckerbauer, G. M.; Eaglesome, M. D.; Samagh, B. S.; Singh, E. L.; Hare, W. C. D.; Randall, G. C. B. Attempts to isolate bovine leukemia and bovine syncytial viruses from blood, uterine flush fluid, unfertilized ova and embryos from infected donor cattle. Ann. Rec. Vet. 12: 385–395; 1981.
Betteridge, K. J. Techniques and results in cattle: Superovulation. Betteridge, K. J. ed. Embryo transfer in farm animals. Canada Department of Agriculture. Monograph 16; 1977; 1–5.
Betteridge, K. J.; Eaglesome, M. E.; Randall, G. C. B.; Mitchell, D. Collection, description and transfer of embryos from cattle 10–16 days after oestrus. J. Reprod. Fertil. 59: 205–216; 1980.
Van der Maaten, M. J.; Miller, J. M. Use of a continuous feline cell line for virologic and serologic investigations of bovine leukemia virus infections. Am. J. Vet. Res. 41: 1785–1788; 1980.
Shannon, J. E.; Macy, M. L. Freezing, storage and recovery of cell stocks. Kruse, P. F.; Patterson, M. K., Jr. eds. Tissue culture. methods and application. New York: Academic Press; 1973: 712–718.
Van Der Maaten, M. J.; Malmquist, W. A. Elimination ofTrypanosoma theileri from cell cultures by amphotericin b treatment. Am. J. Vet. Res. 30: 2013–2015; 1969.
Bouillant, A. M. P.; Genest, P.; Greig, A. S. Non tumoral, benign and malignant stages of transformation of a diploid pig cell line. A review. Can. J. Comp. Med. 45: 279–290; 1981.
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Bouillant, A.M.P., Miller, S.A. & Vineer, W.L.J. Recovery of lymphocytes and epithelioid cells from fluids used to flush the uteri and oviducts of superovulated cattle and preparation of monolayer cultures from epithelial cells of oviduct and uterus. Journal of Tissue Culture Methods 7, 59–62 (1982). https://doi.org/10.1007/BF01665910
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DOI: https://doi.org/10.1007/BF01665910