Summary
Epithelial-cell enriched primary cultures were established from canine prostate by a collagenase digestion and selective attachment procedure. The epithelial cells in primary culture retained differentiated structure and function. The epithelial cells were attached to one another by tight junctions and desmosomes to form “lumenlike structures” that resemble the acini of the intact organ and appeared to contain typical protein synthetic organelles (1,2). The cultures contained significant levels of acid phosphatase and ornithine decarboxylase (2) and retained the ability to metabolize testosterone to dihydrotestosterone and other 5α-reduced metabolites (2–4).
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Douglas, W. H. J.; Terracio, L.; Pennachio, D.; Ofner, P. Primary cultures of canine prostatic epithelial cells. In Vitro 16: 213; 1980.
Terracio, L.; Douglas, W. H. J.; Pennachio, D.; Vena, R. L.; Ofner, P. Primary epithelial cell cultures derived from canine prostate: Isolation, culture and characterization. Am. J. Anat. 164: 311–332; 1982.
Ofner, P.; Terracio, L.; Vena, R.; Douglas, W. H. J. C19-steroid metabolism by epithelial-cell cultures of rat ventral and canine prostate (abstr.). Sixth International Congress of Endocrinology; 1979: 534.
Ofner, P.; Terracio, L.; Vena, R. L.; Douglas, W. H. J. Comparative C19-steroid metabolism by fibroblast and epithelial-cell cultures derived from rat ventral prostate and canine prostate (a bstr.). 67th Society of Endocrinology. 1980: 258.
Rivenson, A.; Silberman, J. The prostatic carcinoma in laboratory animals: A bibliographic survey from 1900 to 1977. Invest. Urology, 16: 458–472; 1979.
Leav, I.; Ling, G. Adenocarcinoma of the canine prostate. Cancer 22: 1329–1334; 1968.
Chevalier, S.; Bleau, G.; Roberts, K. D.; Chapdelaine, A. Characterization of canine prostatic cells from normal and hyperplastic glands. Mol. Cell. Endocrinol. 20: 59–70; 1980.
Webber, M. M. Normal and benign human prostatic epithelium in culture. I. Isolation. In Vitro 15: 967–982; 1979.
Douglas, W. H. J.; Terracio, L.; Glass, H. Isolation, culture and characterization of epithelial cells derived from rat ventral prostate. Anat. Rec. 197: 239–256; 1980.
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Terracio, L., Douglas, W.H.J. Primary culture of canine prostate epithelial cells. Journal of Tissue Culture Methods 7, 51–54 (1982). https://doi.org/10.1007/BF01665908
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DOI: https://doi.org/10.1007/BF01665908