Abstract
This chapter focuses on primary cultures of the human malignant prostate. Current abilities to isolate and culture stem cells, transit-amplifying cells, and secretory luminal cells are described. Advantages and limitations of this model system are also discussed.
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References
Ellis LM, Fidler IJ (2010) Finding the tumor copycat. Therapy fails, patients don’t. Nat Med 16:974–975
Peehl DM, Wong ST, Stamey TA (1988) Clonal growth characteristics of adult human prostatic epithelial cells. In Vitro 24:530–536
Hudson DL, O’Hare M, Watt FM, Masters JRW (2000) Proliferative heterogeneity in the human prostate: evidence for epithelial stem cells. Lab Investig 80:1243–1250
Collins AT, Habib FK, Maitland NJ, Neal DE (2001) Identification and isolation of human prostate epithelial stem cells based on α2β1-integrin expression. J Cell Sci 114:3865–3872
Birnie R, Bryce SD, Roome C et al (2008) Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions. Genome Biol 9:R83. https://doi.org/10.1186/gb-2008-9-5-r83
Robinson EJ, Neal DE, Collins AT (1998) Basal cells are progenitors of luminal cells in primary cultures of differentiating human prostatic epithelium. Prostate 37:149–160
Richardson GD, Robson CN, Lang SH, Neal DE, Maitland NJ, Collins AT (2004) CD133, a novel marker for human prostatic epithelial stem cells. J Cell Sci 117:3539–3545
Brawer MK, Peehl DM, Stamey TA, Bostwick DG (1985) Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res 45:3663–3667
Wernert N, Seitz G, Achstätter T (1987) Immunohistochemical investigation of different cytokeratins and vimentin in the prostate from the fetal period up to adulthood and in prostate carcinoma. Pathol Res Pract 182:617–626
Tomlins SA, Rhodes DR, Perner S et al (2005) Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science 310:644–648
Lang SH, Stark M, Collins A, Paul AB, Stower MJ, Maitland NJ (2001) Experimental prostate epithelial morphogenesis in response to stroma and three dimensional matrigel culture. Cell Growth Differ 12:631–640
Collins AT, Berry PA, Hyde C, Stower MJ, Maitland NJ (2005) Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res 65:10946–10951
Cunha GR, Bigsby RM, Cooke PS, Sugimura Y (1985) Stromal-epithelial interactions in adult organs. Cell Differ 17:137–148
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Collins, A.T. (2018). Methodologies Applied to Establish Cell Cultures in Prostate Cancer. In: Culig, Z. (eds) Prostate Cancer. Methods in Molecular Biology, vol 1786. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7845-8_3
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DOI: https://doi.org/10.1007/978-1-4939-7845-8_3
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7845-8
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