Summary
Rat liver chromatin and the DNA derived from it have been transcribed by both pureE. coli RNA polymerase and the chromatin homologous polymerase II. The RNA transcribed from chromatin by the bacterial polymerase hybridizes to 5% of DNA, while that transcribed from DNA hybridizes to 10% of DNA. The sequences transcribed are largely similar as determined by hybridization competition experiments in which largely repetitive sequences are studied.
The RNA transcribed from chromatin by liver polymerase II contains a larger proportion of middle repetitive sequences than that transcribed by bacterial polymerase. The RNA transcribed from DNA by homologous polymerase also contains a higher proportion of repetitive sequences than that transcribed by pureE. coli polymerase. The similarity in the repetitive sequences transcribed by bacterial polymerase from, respectively, chromatin and DNA, makes it impossible to draw conclusions in this system regarding fidelity of chromatin reconstitution and similar matters.
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Dupras, M., Bonner, J. Transcription of rat liver chromatin and DNA by homologous and E. coli RNA polymerase. Mol Cell Biochem 3, 27–33 (1974). https://doi.org/10.1007/BF01660074
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DOI: https://doi.org/10.1007/BF01660074