Summary
-
1.
The characterization and separation of two enoyl-CoA reductases from rat liver with different catalytic properties are described.
-
2.
Studies on the subcellular location revealed both enzymes to be constituents of a mitochondrial and microsomal fatty acid chain elongation system, respectively.
-
3.
The microsomal enzyme shows optimal activity with hexenoyl-CoA, the mitochondrial enoyl-CoA reductase with decenoyl-CoA. The implications of this observation regarding a preference for γ-linolenic acid and linolenic acid, respectively, as the physiological substrates of microsomal and mitochondrial enoyl-CoA reductase will be discussed.
-
4.
The mitochondrial enoyl-CoA reductases from rat liver and heart exhibit different pyridine nucleotide specificities. The implications of these differences concerning important roles in gluconeogenesis or nitrogen metabolism and in conservation of energy, respectively, is discussed.
-
5.
A possible relationship between mitochondrial fatty acid chain elongation in the aorta and the pathogenesis of atherosclerosis is discussed.
Similar content being viewed by others
References
G. R. Langdon, J. Am. Chem. Soc. 77, 5190 (1955).
W. Seubert, G. Greull, F. Lynen, Angew. Chem. 69, 359 (1957).
W. Seubert, I. Lamberts, R. Kramer, B. Ohly, Biochim. Biophys. Acta 164, 498 (1968).
E. R. Podack, W. Seubert, Biochim. Biophys. Acta 280, 235 (1972).
S. J. Wakil, L. W. McLain, J. B. Warshaw, J. Biol. Chem. 235, PC31 (1960).
S. J. Wakil, J. Lipid Res. 2, 1 (1961).
W. R. Harlan, S. J. Wakil, Biochem. Biophys. Res. Comm. 8, 131 (1962).
W. R. Harlan, S. J. Wakil, J. Biol. Chem. 238, 3216 (1963).
P. W. Holloway, S. J. Wakil, J. Biol. Chem. 239, 2489 (1964).
S. C. Boone, S. J. Wakil, Biochemistry 9, 1470 (1970).
E. J. Barron, Biochim. Biophys. Acta 116, 425 (1966).
L. A. Mooney, E. J. Barron, Biochem. 9, 2138 (1970).
E. J. Barron, L. A. Mooney, Biochem. 9, 2143 (1970).
W. Colli, P. C. Hinkle, M. E. Pullman, J. Biol. Chem. 214, 6432 (1969).
W. E. Donaldson, E. M. Wit-Peeters, H. R. Scholte, Biochim. Biophys. Acta 202, 35 (1970).
C. F. Howard, Biochim. Biophys. Acta 164, 448 (1968).
E. Quagliariello, C. Landriscina, P. Coratelli, Biochim. Biophs. Acta 161, 12 (1968).
A. F. Whereat, F. E. Hull, M. W. Orishimo, J. L. Rabinowitz, J. Biol. Chem. 242, 4013 (1967).
F. E. Hull, A. F. Whereat, J. Biol. Chem. 242, 4023 (1967).
A. F. Wherat, M. W. Orishimo, J. Nelson, S. J. Phillips, J. Biol. Chem. 244, 6498 (1969).
A. F. Whereat in R. Paoletti and D. Kritchevsky, Adv. in Lipid Research, Vol. 9, Academic Press, New York and London 1971, p. 119.
E. M. Wit-Peeters, H. R. Scholte, H. L. Elenbaas, Biochim. Biophys. Acta. 210, 360 (1970).
W. C. Hülsmann, Biochim. Biophys. Acta 45, 623 (1960).
W. C. Hülsmann, Biochim. Biophys. Acta 58, 417 (1962).
W. C. Hülsmann, D. S. Dow, Biochim. Biophys. Acta 84, 486 (1964).
W. S. Hülsmann, Biochim. Biophys. Acta 125, 397 (1966).
E. J. Christ, W. C. Hülsmann, Biochim. Biophys. Acta 60, 72 (1962).
E. J. V. J. Christ, Biochim. Biophys. Acta 152, 50 (1968).
J. V. Dahlen, J. W. Porter, Arch. Biochem. Biophys. 127, 207 (1968).
J. Ghosal, T. Witworth, J. G. Coniglio, Biochim. Biophys. Acta 187, 576 (1969).
E. M. Wit-Peeters, Biochim. Biophys. Acta 176, 453 (1969).
E. Aeberhard, J. H. Menkes, J. Biol. Chem. 243, 3843 (1968).
C. F. Howard, J. Lipid Res. 9, 254 (1968).
J. Filipovic, E. Buddecke, Eur. J. Biochem. 20, 587 (1971).
A. F. Whereat, J. Lipid Res. 7, 671 (1966).
J. Filipovic, M. Rutemöller, E. Buddecke, Z. Physiol. Chem. 353, 1512 (1972).
W. Stoffel, Hoppe Seyler's Z. Physiol. Chem. 333, 71 (1963).
W. Stoffel, K. L. Ach, Hoppe Seyler's Z. Physiol. Chem. 337, 123 (1964).
W. Stoffel, E. Schütte, Lipoide, 16. Colloquium der Gesellschaft für Physiologische Chemie am 28./30. April 1965 in Mosbach/Baden, Springer Verlag, Berlin, Heidelberg, New York, 1966, p. 64.
D. H. Nugteren, Biochem. J. 89, 28P (1963).
D. H. Nugteren, Biochim. Biophys. Acta 106, 280 (1965).
R. B. Guchhait, G. R. Putz, J. W. Porter, Arch. Biochem. Biophys. 117, 541 (1966).
S. Abraham, E. Lorch, I. L. Chaikoff, Biochem. Biophys. Res. Comm. 7, 190 (1962).
S. Abraham, I. L. Chaikoff, W. Bortz, H. P. Klein, H. Den, Nature 192, 1287 (1961).
E. Lorch, S. Abraham, I. L. Chaikoff, Biochim. Biophys. Acta 70, 627 (1963).
H. Mohrhauer, K. Christiansen, M. V. Gan, M. Denbig, R. T. Holmann, J. Biol. Chem. 242, 4507 (1967).
K. Christiansen, Y. Marcel, M. N. Gan, H. Mohrhauer, R. T. Holmann, J. Biol. Chem. 243, 2969 (1968).
R. R. Brenner, R. O. Peluffo, Biochim. Biophys. Acta 176, 471 (1969).
C. Landriscina, G. V. Gnoni, E. Quagliariello, Biochim. Biophys. Acta 202, 405 (1970).
E. R. Podack, Doctoral Thesis, Medical Faculty, Göttingen 1971.
E. R. Podack, G. Freyer, W. Seubert: in preparation.
P. B. Garland, D. Shephard, D. W. Yates, Biochem. J. 97, 587 (1965).
E. Klenk, H. Mohrhauer, Z. Physiol. Chem. 370, 218 (1960).
J. F. Mead, Fed. Proc. 20, 921 (1961).
B. Chance, J. R. Williams, Adv. Enzymol. 17, 65 (1956).
W. Seubert, B. Ohly-Müller: unpublished data.
E. R. Podack, H. P. Schröder, W. Seubert: unpublished data.
F. E. Hull, R. A. Waugh, M. Malone, G. Albers-Schönberg, J. Smith, Archives Biochem. Biophys. 149, 69 (1972).
H. Kanoh, D. B. Lindsay, Biochem. J. 128, 847 (1972).
M. Klingenberg, W. Glenczka, Biochem. Z. 331, 486 (1959).
M. Klingenberg, D. Pette, Biochem. Biophys. Res. Commun. 7, 430 (1962).
J. M. Rager, S. Papa, Biochim. Biophys. Acta 99, 570 (1965).
S. Papa, J. M. Tager, A. Francavilla, E. J. De Haan, E. Quagliariello, Biochim. Biophys. Acta 131, 14 (1967).
R. W. St. Clair, H. B. Lafland, T. B. Carlson, J. Lipid Res. 9, 739 (1968).
G. H. Rothblat, D. Kritchevsky, Biochim. Biophys. Acta 144, 423 (1967).
W. Kaschka, W. Seubert: unpublished results.
Author information
Authors and Affiliations
Additional information
Dedicated to ProfessorTheodor Wieland on occasion of his sixtieth birthday.
an invited article
Rights and permissions
About this article
Cite this article
Seubert, W., Podack, E.R. Mechanisms and physiological roles of fatty acid chain elongation in microsomes and mitochondria. Mol Cell Biochem 1, 29–40 (1973). https://doi.org/10.1007/BF01659936
Issue Date:
DOI: https://doi.org/10.1007/BF01659936