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The role of mitochondria in modifying the cellular inoc environment: Studies of the kinetic accumulation of calcium by rat liver mitochondria

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Abstract

  1. 1.

    The affinity of ATP-supported Ca2+ accumulation for both Ca2+ and ATP was determined from initial rate studies employing isolated rat liver mitochondria. TheK m values for “free” Ca2+ and ATP were calculated to be of the order of 2 μM and 100 μM, respectively. TheK m for ATP decreased as the Ca2+ concentration was increased.

  2. 2.

    The curve relating initial rates of Ca2+ accumulation to Ca2+ concentration was singmoidal in shape; values obtained for the Hill coefficient were in the range 1.5–1.9.

  3. 3.

    Concomitant with the ATP-stimulated accumulation of Ca2+, ATP translocation was itselt increased in the presence of Ca2+. This stimulation took place independently of Ca2+ accumulation.

  4. 4.

    Decreasing the pH of the incubation medium decreased the rate of Ca2+ accumulation. This inhibition was competitive in that the affinity of mitochondrial for Ca2+ could be altered. The maximal rate of accumulation did not change with change in pH.

  5. 5.

    The permeant anions inorganic phosphate and acetate stimulated the accumulation of Ca2+ in a non-competitive manner. Both theV max and Km varied when either of the anions were present.

  6. 6.

    The data are discussed in relation to the role that mitochondria play in controlling the cellular ionic environment.

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Abbreviations

K m :

the substrate concentration at 1/2Vimmax

n :

the Hill coefficient, is the negative slope of a plot of logV max/v-1 versus longs, and substrate concentration at velocityv

HEPES:

N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Austrilian National University, Camberra, A.C.T., Australia

    Terry Spencer & Fyfe L. Bygrave

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  1. Terry Spencer
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  2. Fyfe L. Bygrave
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Spencer, T., Bygrave, F.L. The role of mitochondria in modifying the cellular inoc environment: Studies of the kinetic accumulation of calcium by rat liver mitochondria. J Bioenerg Biomembr 4, 347–362 (1973). https://doi.org/10.1007/BF01648977

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  • DOI: https://doi.org/10.1007/BF01648977

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