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Aggravation by morphine and D-aspartic acid of pyelonephritis induced by i. v. inoculation of staphylococcus aureus in rats

Aggravierung der durch Staphylococcus aureus bei Ratten ausgelösten Pyelonephritis durch Morphin und D-Aspartinsäure

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Summary

Experimental pyelonephritis was induced by intravenous inoculation ofStaphylococcus aureus in homozygous Brattleboro diabetes insipidus (Hom Brattleboro DI), heterozygous Brattleboro (Het Brattleboro) and Wistar rats. One group of rats from each strain was implanted with morphine-containing pellet three days before inoculation. Another series of groups received D-aspartic acid (D-ASP) intraperitoneally, starting three days before inoculation throughout the experiments. Owing to the inhibition by morphine or D-ASP of food intake, another control group from each strain was subjected to food restriction. Pyelonephritis development on the tenth day of inoculation was evaluated by the determination of viable bacteria in urine and total kidney tissue, and pathomorphological lesions in kidney. Hom Brattleboro DI rats appeared more resistant. Morphine or D-ASP significantly increased the findings in three strains of rat.

Zusammenfassung

Durch intravenöse Inokulation vonStaphylococcus aureus wurde bei homozygoten Brattleboro Diabetes insipidus (Hom Brattleboro DI)-Ratten, heterozygoten Brattleboro (Het Brattleboro)- und bei Wistar-Ratten eine experimentelle Pyelonephritis ausgelöst. Einer Gruppe von Ratten von jedem Stamm wurde drei Tage vor der Inokulation eine Morphin-gefüllte Kapsel implantiert. Eine weitere Reihe von Gruppen erhielt vom dritten Tag vor der Inokulation an während der gesamten Experimente D-Aspartinsäure intraperitoneal verabreicht. Da durch Morphin oder D-Aspartinsäure die Nahrungsaufnahme beeinträchtigt wird, wurde eine weitere Kontrollgruppe von jedem Stamm auf reduzierte Nahrungszufuhr gesetzt. Die Entwicklung der Pyelonephritis am zehnten Tag nach der Inokulation wurde durch Bestimmung der Zahl lebensfähiger Bakterien im Urin und im gesamten Nierengewebe und der pathologischen Herde in der Niere bestimmt. Hom-Brattleboro-DI-Ratten erschienen resistenter. Unter dem Einfluß von Morphin oder D-ASP fanden sich in allen drei untersuchten Rattenstämmen signifikant stärker ausgeprägte pathologische Befunde.

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Authors and Affiliations

  1. Department of Pharmacology, Istanbul Medical Faculty, Çapa, Istanbul, Turkey

    H. Koyuncuoğlu,  M. Güngör &  H. Sağduyu

  2. Department of Microbiology, Istanbul Medical Faculty, Çapa, Istanbul, Turkey

    Ö. Anğ

  3. Department of Pathology, Istanbul Medical Faculty, Çapa, Istanbul, Turkey

    V. Uysal

  4. Department of Microbiology, Istanbul Faculty of Dentistry, Çapa, Istanbul, Turkey

    D. Inanç &  M. Anğ-Küçüker

Authors
  1. H. Koyuncuoğlu
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  2. M. Güngör
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  3. H. Sağduyu
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  4. Ö. Anğ
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  5. V. Uysal
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  6. D. Inanç
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  7. M. Anğ-Küçüker
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Koyuncuoğlu, H., Güngör, M., Sağduyu, H. et al. Aggravation by morphine and D-aspartic acid of pyelonephritis induced by i. v. inoculation of staphylococcus aureus in rats. Infection 16, 42–45 (1988). https://doi.org/10.1007/BF01646931

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  • DOI: https://doi.org/10.1007/BF01646931

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