Summary
The structural basis for the cross-reactivity between theEscherichia coli K13, K20 and K23 capsular polysaccharides is the →)-β-ribofuranosyl-(1→7)-β-2-keto-3-deoxyoctonate polymer. Monoclonal anti-bodies againstE. coli K13 which require O-acetyl-2-keto-3-deoxyoctonate for binding were further investigated. Such antibodies, of both the IgG and the IgM isotype, opsonizedE. coli K13in vitro and protected against intraperitoneal infection in mice as well as ascending pyelonephritis in rats. A monoclonal IgG1 anti-idiotype, specific for the K13 polysaccharide combining site of a protective IgM idiotype, primed for protection against intraperitoneal infection with liveE. coli K13 following K13 injections at four as well as 12 weeks of age. the K13 polysaccharide alone did not immunize and protect. The monoclonal anti-K13 idiotype only primed for protection at four weeks of age. These findings suggest a strong effect of a single idiotype on the outcome of a bacterial infection.
Zusammenfassung
Das →)-β-ribofuranosyl-(1→7)-β-2-keto-3-deoxyoctonat-Polymer stellt die strukturelle Basis für die Kreuzreaktivität zwischen denEscherichia coli K13-, K20- und K23-Kapselpolysacchariden dar. Es wurden Untersuchungen an monoklonalen Antikörpern gegenE. coli K13 durchgeführt, die für die Bindung O-acetyl-2-keto-3-deoxyoctonat benötigen. Solche Antikörper, sowohl vom IgG- wie vom IgM-Isotyp, opsoniertenE. coli K 13in vitro und besaβen protektive Witkung gegen die intraperitoneale Infektion bei Mäusen und die aszendierende Pyelonephritis bei Ratten. Ein monoklonaler IgG1 anti-Idiotyp mit Spezifität gegen die K13-Polysaccharid-Bindungsstelle eines protektiven IgM-Idiotypen prägte eine Protektion gegen die intraperitoneale Infektion mit lebendenE. coli K13 nach K13-Injektion im Alter von vierundvon 12Lebenswochen. Das K13-Polysaccharid allein führte nicht zur Immunisierung und Protektion. Der monoklonale Anti-K13-Idiotyp prägte nur im Alter von vier Lebenswochen eine Protektion. Aus diesen Befunden läßt sich ableiten, daß ein einzelner Idiotyp den Ausgang einer bakteriellen Infektion wesentlich beeinflußt.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature
Kaijser, B., Hanson, L. Å., Jodal, U., Lidin-Janson, G., Robbins, J. B. Frequency ofE. coli K antigens in urinary tract infections in children. Lancet I (1977) 663–664.
Robbins, J. B. Vaccines for the prevention of encapsulated bacterial diseases: current status, problems and prospects for the future. Immunochemistry 15 (1978) 839–854.
Jann, K., Jann, B. The K antigens ofEscherichia coli. In:Hanson, L. Å., Kallós, P., Westphal, O. (eds.): Host parasite relationships in gram-negative infections. Progress in Allergy 33, Karger, Basel 1983, pp. 53–79.
Vann, W. F., Söderström, T., Egan, W., Tsui, F.-P., Schneerson, R., Ørskov, I., Ørskov, F. Serological, chemical and structural analysis of theEscherichia coli cross-reactive capsular polysaccharides K13, K20 and K23. Infect. Immun. 39 (1983) 623–629.
Ørskov, I., Ørskov, F., Jann, B., Jann, K. Serology, chemistry, and genetics of O and K antigens ofEscherichia coli. Bacteriol. Rev. 41 (1977) 667–710.
Söderström, T., Stein, K., Brinton, Jr., C. C., Hosea, S., Burch, C., Hansson, H. A., Karpas, A., Scheerson, R., Sutton, A., Vann, W. J., Hanson, L. Å. Serological and functional properties of monoclonal antibodies toEscherichia coli type 1 pilus and capsular antigens. In:Hanson, L. Å., Kallós, P., Westphal, O. (eds.): Host parasite relationships in gram-negative infections. Progress in Allergy 33, Karger, Basel 1983, pp. 259–274.
Engvall, E., Perlmann, P. Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immuno-globulin in antigen-coated tubes. J. Immunol. 109 (1972) 129–135.
Stein, K. E., Bona, C., Lieberman, R., Chien, C. C., Paul, W. Regulation of the anti-inulin antibody response by a non allotypelinked gene. J. Exp. Med. 151 (1980) 1088–1102.
Weeke, E. Crossed immunoelectrophoresis. Scand. J. Immunol. 2 Suppl. (1973) 47–56.
Söderström, T., Cole, M. F., Dahlgren, U., Hou, S. D., Schneerson, R., Sutton, A., Hanson, L. Å.: The murine serum and secretory immune response againstEscherichia coli capsular polysaccharide and type 1 pili. (Submitted for publication).
Söderström, T., Öhman, L.: The effect of monoclonal antibodies againstEscherichia coli type 1 pili and capsular polysaccharides on the interaction between bacteria and human granulocytes. Scand. J. Immunol. (in print).
Old, D. C. Inhibition of the interaction between fimbrial hemagglutination and erythrocytes by D-mannose and other carbohydrates. J. Gen. Microbiol. 71 (1972) 149–157.
Öhman, L., Magnusson, K.-E., Stendahl, O. The mannose-specific lectin activity ofEscherichia coli type 1 fimbriae, assayed by agglutination of glycolipid-containing liposomes, erythrocytes, and yeast cells and hydrophobic interaction chromatography. FEMS Microbiol. Lett. 14 (1982) 149–153.
Öhman, L., Hed, J., Stendahl, O. Interaction between human polymorphonuclear leucocytes and two different strains of type 1 fimbriae-bearingEscherichia coli. J. Infect. Dis. 146 (1982) 751–757.
Kaijser, B., Ahlstedt, S. Protective capacity of antibodies againstEscherichia coli O and K antigens. Infect. Immun. 17 (1977) 286–289.
Söderström, T., Stein, K. E.: Neonatal administration of idiotype or anti-idiotype primes for protection againstE. coli K13 infection in mice. J. Exp. Med. (in print).
Söderström, T., Brinton, Jr., C. C., Fusco, P., Karpas, A., Ahlstedt, S., Stein, K., Sutton, A., Hosea, S., Schneerson, R., Hanson, L. Å. In:Schlessinger, D. (ed.): Microbiology 1982. American Society for Microbiology, Washington, D.C. 1982, pp. 305–307.
Kaijser, B., Larsson, P., Nimmich, W., Söderström, T. Antibodies toEscherichia coli K and O antigens in protection against acute pyelonephritis. In:Hanson, L. Å., Kallós, P., Westphal, O. (eds.): Host parasite relationships in gram-negative infections. Progress in Allergy 33, Karger, Basel 1983, pp. 275–288.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Söderström, T., Hanson, L., Öhman, L. et al. Studies on immunity against escherichia coli K13 with monoclonal Anti-K13 and Anti-anti-K13. Infection 13 (Suppl 2), S256–S259 (1985). https://doi.org/10.1007/BF01644440
Issue Date:
DOI: https://doi.org/10.1007/BF01644440