Skip to main content

Advertisement

SpringerLink
Log in

Protection of immunosuppressed mice against infection with Pseudomonas aeruginosa by monoclonal antibodies to outer membrane protein OprI

Protektion immunsupprimierter Mäuse gegen Pseudomonas aeruginosa durch monoklonale Antikörper gegen äußeres Membranprotein I (Opr I)

  • Originalia
  • Published:
Infection Aims and scope Submit manuscript

Summary

Two monoclonal antibodies (mAbs) designated 2A1 and 6A4 which had been shown to be directed againstPseudomonas aeruginosa outer membrane lipoprotein I were tested in cyclophosphamide treated mice for their protective ability againstP. aeruginosa infection. Pretreatment of mice with either 2 mg of 2A1 or 4 mg of 6A4 in combination with lethally irradiated human leucocytes reduced the mortality after subsequent subcutaneous injection of 100 livingP. aeruginosa organisms to 50% of the controls. Without leucocytes only mAb 2A1 (isotype IgG2b) showed protection (47%). Irradiated leucocytes alone without mAb did not protect the mice significantly from fatalP. aeruginosa infection.

Zusammenfassung

Zwei monoklonale Antikörper (2A1, 6A4) gegen Lipoprotein I vonPseudomonas aeruginosa wurden auf ihre protektive Wirkung gegenP. aeruginosa-Infektionen in mit Cyclophosphamid behandelten Mäusen getestet. Die Gabe von 2 mg 2A1 oder 4 mg 6A4 zusammen mit bestrahlten (25 Gy) humanen Leukozyten reduzierte die Mortalität der Mäuse nach Injektion von 100 lebendenP. aeruginosa-Keimen auf 50% der Kontrollen. Ohne Leukozyten zeigte nur der Antikörper 2A1 (Isotyp IgG2b) eine protektive Wirkung (47%). Mit 25 Gy bestrahlte Leukozyten alleine zeigten keine protektive Wirkung.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Masur, H., Cheigh, J. S., Stubenbord, W. T. Infection following renal transplantation: a changing pattern. Rev. Infect. Dis. 4 (1982) 1208–1219.

    Google Scholar 

  2. Young, L. S. Management of infections in leukemia and lymphoma. In:Rubin, R. H., Young, L. S., (eds): Clinical approach to infection in the compromised host. Plenum Medical Book Company, New York and London 1988. pp. 467–501.

    Google Scholar 

  3. Gottlieb, M. S. Acquired immunodeficiency syndrome. In:Rubin, R. H., Young, L. S. (eds.): Clinical approach to infection in the compromised host. Plenum Medical Book Company, New York and London 1988, pp. 381–407.

    Google Scholar 

  4. Hancock, R. E. W. Intrinsic antibiotic resistance ofPseudomonas aeruginosa. J. Antimicrob. Chemother. 18 (1986) 653–659.

    Google Scholar 

  5. Zak, O. Antibiotics andPseudomonas aeruginosa. In:Sabath, L. D. (ed.):Pseudomonas aeruginosa, International Symposium, Huber Bern 1980, pp. 133–159.

    Google Scholar 

  6. Mutharia, L. M., Nicas, T. I., Hancock, R. E. W. Outer membrane proteins ofPseudomonas aeruginosa serotype strains. J. Infect. Dis. 146 (1982) 770–779.

    Google Scholar 

  7. Duchêne, M., Schweizer, A., Lottspeich, F., Krauss, G., Marget, M., Vogel, K., v. Specht, B.-U., Domdey, H. Sequence and transcriptional start site of thePseudomonas aeruginosa outer membrane porin protein F gene. J. Bacteriol. 170 (1988) 155–162.

    Google Scholar 

  8. Duchêne, M., Barron, C., Schweizer, A., v. Specht, B.-U., Domdey, H. Pseudomonas aeruginosa outer membrane lipoprotein I gene: molecular cloning, sequence, and expression inEscherichia coli. J. Bacteriol. 171 (1989) 4130–4137.

    Google Scholar 

  9. v. Specht, B.-U., Strigl, G., Ehret, W., Brendel, W. Protective effect of an outer membrane vaccine againstPseudomonas aeruginosa infection. Infection 15 (1987) 408–412.

    Google Scholar 

  10. Fisher, M: W. A polyvalent human γ-globulin toPseudomonas aeruginosa: passive protection of mice against lethal infection. J. Infect. Dis. 136 (1977) 111–115.

    Google Scholar 

  11. Hancock, R. E. W., Mutharia, L. M., Muvat, E. C. A. Immunotherapeutic potential of monoclonal antibodies againstPseudomonas aeruginosa protein F. Eur. J. Clin. Microbiol. 4 (1985) 224–227.

    Google Scholar 

  12. Jones, C. E., Alexander, W., Fisher, M. Clinical evaluation ofPseudomonas hyperimmune globulin. J. Surg. Res. 14 (1973) 87–96.

    Google Scholar 

  13. Pennington, J. E., Small, G. T., Lostrom, M. E., Pier, G. B. Polyclonal and monoclonal antibody therapy for experimentalPseudomonas aeruginosa pneumonia. Infect. Immun. 56 (1986) 239–244.

    Google Scholar 

  14. Sawada, S., Kawamura, T., Masuho, Y. Immunoprotective human monoclonal antibodies against five major serotypes ofPseudomonas aeruginosa. J. Gen. Microbiol. 133 (1987) 3581–3590.

    Google Scholar 

  15. Sawada, S., Suzuki, M., Kawamura, T., Fujinaga, S., Masuho, Y., Tomibe, K. Protection against infection withPseudomonas aeruginosa by passive transfer of monoclonal antibodies to lipopolysaccharides and outer membrane proteins. J. Infect. Dis. 150 (1984) 570–576.

    Google Scholar 

  16. Zweerink, H. J., Gammon, M. C., Hutchison, C. F., Jackson, J. J., Lombardo, D., Miner, K. M., Puckett, J. M., Sewell, T. J., Sigal, N. H. Human monoclonal antibodies that protect mice against challenge withPseudomonas aeruginosa. Infect. Immun. 56 (1988) 1873–1879.

    Google Scholar 

  17. Heiss, M. M., v. Specht, B.-U., Beckert, A., Ehret, W., Brendel, W. Biological and potential therapeutic properties of monoclonal antibodies againstPseudomonas aeruginosa. In:Schriefers, K. H. (ed.): Chirurgisches Forum '88 f. experim. u. klin. Forschung, Springer Berlin, Heidelberg, 1988, pp. 317–322.

    Google Scholar 

  18. Cryz, J. R., Fürer, S. C. E., Germanier, R. Passive protection againstPseudomonas aeruginosa infection in an experimental leucopenic mouse model. Infect. Immun. 40 (1983) 659–664.

    Google Scholar 

  19. Marget, M., Eckhardt, A., Ehret, W., v. Specht, B.-U., Duchêne, M., Domdey, H. Cloning and characterization of heavy and light chain cDNAs fromPseudomonas aeruginosa outer membrane protein I specific monoloncal antibody. Gene 74 (1989) 335–345.

    Google Scholar 

  20. Kaplan, E. L., Meier, P. Nonparametric estimation from incomplete observations. J. Am. Statist. Assoc. 53 (1958) 457–481.

    Google Scholar 

  21. Peto, R., Peto, J. Asymptotically efficient rank invariant test procedures (with discussion). J. R. Statist. Soc. A (1972) 135–206.

    Google Scholar 

  22. Tegtmeier, R. B., Andersen, B. R. Mechanisms of lipopolysaccharide-induced protection againstPseudomonas sepsis in granulocytopenic mice. Rev. Infect. Dis. 5 (1983) 963–970.

    Google Scholar 

  23. Harvath, L., Andersen, B. R., Amirault, H. J. Passive immunity againstPseudomonas sepsis during granulocytopenia. Infect. Immun. 14 (1976) 1151–1154.

    Google Scholar 

  24. Trautmann, M., Brückner, O., Hahn, H. Protective effect ofPseudomonas-specific immunoglobulin from the rabbit and of active immunization in experimentalPseudomonas septicemia in mice. Zbl. Bakt. Hyg. 252 (1982) 370–383.

    Google Scholar 

  25. Gilleland, Jr. H. E., Parker, M. G., Matthews, M., Berg, R. D. Use of a purified outer membrane protein F (porin) preparation ofPseudomonas aeruginosa as a protective vaccine in mice. Infect. Immun. 44 (1984) 49–54.

    Google Scholar 

  26. Alavi, J. B., Root, R. K., Djerassi, L., Evans, A. E., Gluckman, S. J., McGregor, R. R., Du Pont Guerry, Schreiber, A. D., Shaw, J. M., Koch, P., Cooper, R. A. A randomized clinical trial of granulocyte transfusions for infection in acute leukemia. N. Engl. J. Med. 296 (1977) 706–711.

    Google Scholar 

  27. Dale, D. C., Reynolds, H. Y., Pennington, J. E., Elin, R. J., Herzig, G. P. ExperimentalPseudomonas pneumonia in leucopenic dogs: comparison of therapy with antibiotics and granulocyte transfusions. Blood 47 (1976) 869–876.

    Google Scholar 

  28. Herzig, R. H., Herzig, G. P., Graw, R. G., Bull, M. I., Ray, K. K. Successful granulocyte transfusion therapy for gram-negative septicaemia. N. Engl. J. Med. 296 (1977) 701–705.

    Google Scholar 

  29. Herlin, D., Koprowski, H. IgG2a monoclonal antibodies inhibit human tumor growth through interaction with effector cells. Proc. Natl. Acad. Sci. USA 79 (1982) 4761–4765.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chirurgische Forschung, Chirurgische Universitäts-Klinik, Hugstetterstr. 55, D-7800, Freiburg i. Breisgau

    R. Rahner,  A. Eckhardt &  B. -U. von Specht

  2. Laboratorium für Molekulare Biologie, Genzentrum der Ludwig-Maximilians-Universität München, D-8033, Martinsried, FR Germany

    M. Duchêne &  H. Domdey

Authors
  1. R. Rahner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Eckhardt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. -U. von Specht
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Duchêne
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Domdey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rahner, R., Eckhardt, A., von Specht, B.U. et al. Protection of immunosuppressed mice against infection with Pseudomonas aeruginosa by monoclonal antibodies to outer membrane protein OprI. Infection 18, 242–245 (1990). https://doi.org/10.1007/BF01643398

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01643398

Keywords

Search

Navigation