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Efficacy of intravenous immunoglobulin preparations against viral and bacterial infections in mouse protection tests

Wirksamkeit von intravenösen Immunglobulin-Präparationen gegen virale und bakterielle Infektionen im Mäuseschutzversuch

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Summary

Our investigation indicates that pretreatment of human immunoglobulin for the elimination of the anticomplementary activity is associated with a loss of activity, the extent of which depends on the type of treatment applied. Laboratory preparations of human IgG were tested in a mouse protection assay using influenza A2-Taiwan virus, tetanus toxin andSalmonella typhimurium as the challenge. There was a 7–28% reduction in efficacy in an intravenous 7S preparation in comparison with an untreated 7S IgG. F(ab′)2 fragments showed a 24–65% and Fab fragments an 80–100% reduction in efficacy. Two commercial human 7S products showed approximately 90% efficacy in the Salmonella assay; a commercial, pepsin-treated preparation showed 65–74% efficacy when compared with untreated 7S IgG.

Zusammenfassung

Die hier dargelegten Untersuchungen zeigen, daß die Vorbehandlung humanen Immunglobulins zur Elimination der antikomplementären Aktivität mit einem Wirkungsverlust verbunden ist, dessen Ausmaß von der Art der Vorbehandlung abhängig ist. In Mäuseschutzversuchen wurde gefunden, daß die Wirksamkeit von Laborpräparationen humanen, i. v.-verträglichen Immunglobulins gegen Influenza A2-Taiwan Virus, Tetanustoxin undSalmonella typhimurium um ca. 7–28% bei 7S-Immunglobulin im Vergleich zu unbehandeltem Standard-IgG reduziert wurde. F(ab′)2-Fragmente zeigten 24–65%, Fab-Fragmente 80–100% Verlust der Wirksamkeit. Zwei käufliche, humane 7S IgG-Präparationen für intravenöse Anwendung zeigten im Salmonella-Assay ca. 90% Wirksamheit, ein käufliches, pepsin-behandeltes IgG-Präparat ca. 65–74% der Wirksamkeit eines unbehandelten 7S IgG-Präparates.

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  1. Biotest-Serum-Institut GmbH, Flughafenstraße 4, D-6000, Frankfurt/Main

    W. Stephan &  H. Dichtelmüller

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  1. W. Stephan
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Stephan, W., Dichtelmüller, H. Efficacy of intravenous immunoglobulin preparations against viral and bacterial infections in mouse protection tests. Infection 11, 227–231 (1983). https://doi.org/10.1007/BF01641203

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