Summary
Platelet aggregation by various inductors was studied in citrated and heparinized plasma of the following groups of subjects: Normal, hemophilia A, combined factor V and factor VIII deficiency, v. Willebrand's disease and congenital afibrinogenemia. The results may be summarized as follows:
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A
platelet aggregation incitrated plasma
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1)
platelet aggregation bycommon inductors ADP, adrenalin andcollagen was normal in all groups of subjects but for the patients with congenital afibrinogenemia in whom adrenalin induced aggregation was absent or markedly reduced whereas ADP and collagen gave slightly reduced or near normal aggregation curves.
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2)
platelet aggregation byristocetin was normal in all groups of subjects but for v. Willebrand's disease in which it was absent.
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B
platelet aggregation inheparinized plasma
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1)
platelet aggregation bycommon inductors resulted to be normal in all groups of subjects except in congenital afibrinogenemia. In this latter case the pattern was still mildly defective but here was an increased aggregation as compared to citrated plasma. These findings have been interpreted to indicate a potentiating effect of heparin with regard to common inductors.
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2)
platelet aggregation byristocetin resulted to be absent in all groups of subjects investigated. The possible mechanism of action of the inhibitory effect exercised by heparin with regard to ristocetin is discussed.
Zusammenfassung
Verfasser berichten über die Beziehung zwischen Plättchenfunktion und Störung des plasmatischen Gerinnungssystems. Die Untersuchung wurde im Zitratblut und heparinisiertem Blut mit den Induktoren ADP, Adrenalin, Kollagen einerseits und Ristocetin andererseits an folgenden Krankengruppen durchgeführt: Hämophilie A, kombinierter Faktor V- und Faktor VIII-Mangel, v. Willebrand'sche Krankheit, Kongenitale Afibrinogenämie und normale Personen.
BeiZitratblut war die Plättchenaggregation mit ADP, Adrenalin und Kollagen in allen Gruppen normal (Ausnahme Kongenitale Afibrinogenämie). Mit Ristocetin als Induktor war in dieser Reihe die Plättchenaggregation in allen Gruppen normal (Ausnahme v.-Willebrand-Krankheit).
BeiHeparinblut war bei den Induktoren ADP, Adrenalin und Kollagen die Plättchenaggregation in allen Krankheitsgruppen normal (Ausnahme kongenitale Afibrinogenämie).
Mit Ristocetin im Heparinplasma war keine Plättchenaggregation in allen Gruppen festzustellen. Der mögliche Aktionsmechanismus durch das Heparin wird besprochen.
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This study was supported in part by a grant from the C.N.R. (grant) CT74.00189.04.
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Girolami, A., De Marco, L., Virgolini, L. et al. The effect of heparin on platelet aggregation by common inductors and by ristocetin in congenital bleeding disorders due to factor VIII or fibrinogen defects. Blut 31, 219–227 (1975). https://doi.org/10.1007/BF01633505
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DOI: https://doi.org/10.1007/BF01633505