Summary
Cultures ofChilomonas paramecium in sodium acetate and inorganic salt solutions were examined for contaminating bacteria by cultural and microscopic methods. No bacteria could be found. Qualitative tests for nitrite and nitrate and quantitative estimation of the former indicated thatChilomonas cannot oxidize ammonia to nitrite or nitrate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hall, R. P., andLoefer, J. B., 1936, On the supposed utilization of inorganic nitrogen by the colorless crytomonad flagellate,Chilomonas paramecium. Protoplasma26, pp. 321–330.
Loefer, J. B., 1934, The trophic nature ofChlorogonium andChilomonas. Biol. Bull.66, pp. 1–6.
—, 1935, Effect of certain nitrogen compounds on growth ofChlorogonium andChilomonas. Arch. f. Protist.85, S. 74–86.
— andHall, R. P., 1935, Further attempts to growChilomonas paramecium in inorganic medium. Science81, p. 486.
Mast, S. O., andPace, D. M., 1932, Synthesis of carbohydrates, lipids and proteins from inorganic salts, carbon dioxide and water in the absence of chlorophyll and light. Amer. J. Phys.,101, p. 75.
— —, 1932 a, Synthesis of protoplasm from inorganic compounds in the colorless animal,Chilomonas paramecium. Anat. Rec.54, pp. 101–102.
— —, 1933, Synthesis from inorganic compounds of starch, fats, proteins and protoplasm in the colorless animal,Chilomonas paramecium. Protoplasma20, S. 326–358.
— —, 1936, Why have some investigators been unable to growChilomonas paramecium in inorganic or simple organic solutions ? Science83, pp. 18–19.
Pringsheim, E. G., 1931, Zur Physiologie der saprophytischen Flagellaten. Beiträge z. allg. Bot.2, S. 88–137.
Author information
Authors and Affiliations
Additional information
The writer was fortunate in being able to spend some months in the biological laboratories of theJohns Hopkins University and undertake the work reported here with the assistance of Dr. S. O.Mast and Dr. D. M.Pace. He wishes to take this opportunity to acknowledge his indebtedness to Dr.Mast and Dr.Pace without whose help it would have been impossible to carry on this work.