Skip to main content

Advertisement

SpringerLink
Log in

Lack of effect of ovariectomy on divalent cation regulation of skeletal adenylate cyclase

  • Original Article
  • Published:
Osteoporosis International Aims and scope Submit manuscript

Abstract

Both estrogen and androgen have been reported to attenuate cyclic AMP responses to parathyroid hormone stimulation in cultured bone cells. The present study examines the effect of in vivo estrogen deficiency on skeletal adenylate cyclase (AC) activity. The AC activity was compared in bone membranes prepared from normal female guinea pigs and from age-matched guinea pigs 3 weeks after ovariectomy. Histomorphometric analysis of femoral specimens from the ovariectomized guinea pigs demonstrated significant decreases in percentage bone volume, the percentage eroded surfaces and osteoclast numbers, and increased osteoid thickness, compared with the normal controls. No differences were found in basal AC activity, the ability of bone AC to be stimulated by parathyroid hormone (bPTH(1–34)) or isoproterenol, or in the regulation of AC activity by calcium and magnesium. We conclude that bone AC is not a direct target for estrogen effects on bone cells and that the reported effects of sex steroids on cAMP levels in bone cells probably act via an indirect mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lindsay R, MacLean A, Kraszewski A, Hart DM, Clark AC, Garwood J. Bone response to termination of estrogen treatment. Lancet 1978;1:1325–7.

    Google Scholar 

  2. Ettinger B, Genant HK, Cann CE. Postmenopausal bone loss is prevented by treatment with low-dosage estrogen with calcium. Ann Intern Med 1987;106:40–5.

    Google Scholar 

  3. Ericksen EF, Colvard DS, Berg NJ et al. Evidence of estrogen receptors in normal human osteoblast-like cells. Science 1988;241:84–6.

    Google Scholar 

  4. Orimo HO, Fujita T, Yoshikawa M. Increased sensitivity of bone parathyroid hormone in ovariectomized rats. Endocrinology 1972;90:760.

    Google Scholar 

  5. Gallagher JC, Nordin BEC. Treatment with estrogens of primary hyperthyroidism in postmenopausal women. Lancet 1973;1:503–7.

    Google Scholar 

  6. Heaney RP. A unified concept of osteoporosis. Am J Med 1965;39:877–80.

    Google Scholar 

  7. Fukayama S, Tashjian AH, Jr. Direct modulation by estradiol of the response of human bone cells (SaOS-2) to human parathyroid hormone (PTH) and PTH-related protein. Endocrinology 1989;124:397–401.

    Google Scholar 

  8. Fukayama S, Tashjian AH, Jr. Direct modulation by androgens of the response of human bone cells (SaOS-2) to human parathyroid hormone (PTH) and PTH-related protein. Endocrinology 1989;125:1789–94.

    Google Scholar 

  9. Ernst M, Heath JK, Rodan GA. Estradiol effects on proliferation, messenger ribonucleic acid for collagen and insulin-like growth factor-I, and parathyroid hormone-stimulated adenylate cyclase activity in osteoblastic cells from calvariae and long bones. Endocrinology 1989;125:825–33.

    Google Scholar 

  10. Rude RK. Skeletal adenylate cyclase: effect of Mg2+, Ca2+, and PTH. Calif Tissue Int 1985;37:318–23.

    Google Scholar 

  11. Cech SV, Broaddus WC, Maguire ME. Adenylate cyclase: the role of magnesium and other divalent cations. Mol Cell Biochem 1980; 33:67–92.

    Google Scholar 

  12. Gruber HE, Marshall GJ, Nolasco LM et al. Alkaline and acid phosphatase demonstration in human bone and cartilage: effects of fixation intervals and methacrylate embedments. Stain Technol 1988;63:299–306.

    Google Scholar 

  13. Chase LR, Auerbach GD. The effect of parathyroid hormone on the concentration of adenosine 3′,5′-monophosphate in skeletal tissue in vitro. J Biol Chem 1970;245:1520–6.

    Google Scholar 

  14. Lowik CWGM, Van Leeuwen JPTM, Van Der Meer JM, Van Zeeland JK, Scheven BAA, Herrmann-Erlee MPM. A two-receptor model for the action of parathyroid hormone on osteoblasts: a role for intracellular free calcium and cAMP. Cell Calcium 1985;6:311–26.

    Google Scholar 

  15. Gray TK. Estrogens and the skeleton: cellular and molecular mechanisms. J Steroid Biochem 1989;34:285–287.

    Google Scholar 

  16. Takano YT, Roda GA. Direct effect of 17-beta-estradiol on trabecular bone in ovariectomized rats. Proc Natl Acad Sci USA 1990;87:2172–6.

    Google Scholar 

  17. Boland CJ, Fried RM, Tashjian AH. Measurement of cytosolic-free Ca2+ concentration in human and rat osteosarcoma cells: actions of bone resorption-stimulating hormones. Endocrinology 1986;118:980–9.

    Google Scholar 

  18. Yamaguchi DT, Hahn TJ, Akiko IK, Kleeman CR, Muallem S. Parathyroid hormone-activated calcium channels in an osteoblast-like clonal osteosarcoma cell line. J Biol Chem 1987;262:7711–8.

    Google Scholar 

  19. Fukayama S, Tashjian AH, Jr. Stimulation by parathyroid hormone of45Ca2+ uptake in osteoblast-like cells: possible involvement of alkaline phosphatase. Endocrinology 1990;126:1941–9.

    Google Scholar 

  20. Scholf C, Cuthbertson KSR, Gallagher JA et al. Measurement of intracellular Ca2+ in single aequorin-injected and suspension of fura-2-loaded ROS 17/2.8 cells and normal human osteoblasts. Biochem J 1991;274:15–20.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Medicine, Orthopaedic Hospital Bone and Connective Tissue Research Laboratories, and Skeletal Dysplasia Morphology Laboratory, University of Southern California, Los Angeles, California, USA

    S. B. Oldham, H. E. Gruber & R. K. Rude MD

Authors
  1. S. B. Oldham
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. E. Gruber
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. K. Rude MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oldham, S.B., Gruber, H.E. & Rude, R.K. Lack of effect of ovariectomy on divalent cation regulation of skeletal adenylate cyclase. Osteoporosis Int 2, 174–179 (1992). https://doi.org/10.1007/BF01623923

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01623923

Keywords

Search

Navigation