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Nitroblau-Tetrazolium-Reduktionskapazität von neutrophilen Granulocyten

Capacity of nitroblue tetrazolium-reduction of neutrophilic granulocytes

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Summary

A modification of the NBT-reaction is described which allows a differentiation of the NBT uptake and NBT-reduction. By means of this method it has been shown that the capacity of NBT-reduction of neutrophil granulocytes taken from hematologically healthy persons is independent of the probands' age and, moreover, of the presence of acute infections and their etiology. It has been shown, that the increase in the NBT-reduction of neutrophilic granulocytes of newborns and persons with acute bacterial infections results from an increased NBT-ingestion. To give an example of a strongly reduced capacity of NBT-reduction of the neutrophilic granulocytes 2 patients suffering from fatal granulomatous disease in childhood have been examined.

Zusammenfassung

Es wird eine Modifikation des NBT-Testes beschrieben, die eine quantitative Differenzierung der NBT-Aufnahme und NBT-Reduktion erlaubt. Mit dieser Methode wurde gezeigt, daß die NBT-Reduktionskapazität neutrophiler Granulocyten hämatologisch gesunder Personen unabhängig vom Alter der Probanden und unabhängig von akuten Infektionen und deren Ätiologie ist. Es wurde gezeigt, daß die Erhöhung der NBT-Reduktion der neutrophilen Granulocyten von Neugeborenen und Patienten mitakutenbakteriellen Infektionendurcheineerhöhte NBT-Ingestion bedingt ist. Als Beispiel verminderter NBT-Reduktionskapazität der neutrophilen Granulocyten wurden 2 Patienten mit Progressiver Septischer Granulomatose untersucht.

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Literatur

  1. Arrowsmith, D., Morin, R.J.: Oral contraceptives and the NBT-Test. Lancet 1973/I, 148–149

  2. Azimi, P.H., Bodenbender, J.G., Hintz, R.L., Kontras, S.B.: Chronic Granulomatous Disease in Three Female Siblings. JAMA206, 2865–2870 (1968)

    Google Scholar 

  3. Baehner, R.L., Johnston, R.B., Jr., Nathan, D.G.: Comparative Study of the Metabolic and Bactericidal Characteristics of Severaly Glucose-6-Phosphate Dehydrogenase-Deficient Polymorphonuclear Leukocytes and Leukocytes from Children with Chronic Granulomatous Disease. RES12, 150–169 (1972)

    Google Scholar 

  4. Bachner, R.L., Nathan, D.G.: Deficient Glucose Oxidation in the Intakt Leukocytes of Chronic Granulomatous Disease. Blood28, 10.10–1011, 1966

    Google Scholar 

  5. Baehner, R.L., Nathan, D.G.: Leukocyte oxidase: Defective activity in chronic granulomatous disease. Science155, 835–836, 1967

    Google Scholar 

  6. Baehner, R.L., Nathan, D.G.: Quantitative nitroblue tetrazolium test in chronic granulomatous disease. New Engl. J. Med.278, Nr. 18, 971–976, 1968

    Google Scholar 

  7. Bellanti, J.A., Cantz, B.E., Yang, M.C., Thadden, H. v., Schlegel, R.J.: Leukocyte Maturation: G-6-PD Activity and NBT Dye Reduction. Pediat. Res.4, 462 (1970)

    Google Scholar 

  8. Bittner, S.J., Kieff, E., Windhorst, D., Meier, P.: The use of the unstimulated nitroblue tetrazolium test as a routine screening test for bacterial infection in an adult population. Amer. J. Clin. Pathol.60, 843–853, 1973

    Google Scholar 

  9. McCall, C.E., Cooper, M.R., DeChatelet, L.R.: Nitroblue Tetrazolium test. J. Pediat.79, 170–71, 1971

    Google Scholar 

  10. Campos, M.E., Rabinovich, S., Smith, I.M.: The nitroblue tetrazolium test. Arch. Internal. Med. 133, 432–436, 1974

    Google Scholar 

  11. Chandra, R.K., Cope, W.A., Soothill, J.F.: Chronic Granulomatous Disease. Evidence for an Autosomal Mode of Inheritance. Lancet 1969, II, 71–74

  12. Cocchi, P., Mori, S., Becattini, A.: Il test al nitroblu tetrazolium nel prematuro. Minerva Pediatrica23, 185–187 (1971)

    Google Scholar 

  13. Cocchi, P., Mori, S., Becattini, A.: Nitriblue-Tetrazolium Reduction by Neutrophils of Newborn Infants in In-Vitrc Phagocytosis Test. Acta Paediat. Scand.60, 475–478 (1971)

    Google Scholar 

  14. Dejesus, M., Jr., Fikrig, S., Detwiler, T.: Phagocytosis-stimulated nitroblue tetrazolium reduction by platelets. J. Lab. Clin. Med. 80, 117–124 (1972)

    Google Scholar 

  15. Feigin, R.D.: NBT Test in the Diagnosis of Febrile Patients. N. Engl. J. Med.285, 347–348 (1971)

    Google Scholar 

  16. Feigin, R.D., Shackelford, P.G., Choi, S.C., Flake, K.K., Franklin, F.A., Jr., Eisenberg, C.S.: Nitroblue tetrazolium dye test as an aid in the differentail diagnosis of febrile disorders. J. Pediatrics78, 230–237 (1971)

    Google Scholar 

  17. Fikrig, S.M., Berkowich, S., Emmet, S.M., Gordon, C.: Nitroblue Tetrazolium dye test and differential diagnosis of meningitis. J. Pediat.82, 855–857, 1973

    Google Scholar 

  18. Freeman, R., King, B.: Infective Complications of Indwelling Intravenous Catheters and the Monitorin of Infections by the Nitroblue-Tetrazolium Test. Lancet 1972/I, 992–993

  19. Freeman, R., King, B.: N.B.T.-Test in Acute Appendicitis. Lancet 1972/II, 1198

  20. Gifford, R.H., Malawista, S.E.: A simple rapid micromethod for detecting chronic granulomatous disease of childhood. J. Lab. Clin. Med.75, 511–519, 1970

    Google Scholar 

  21. Glyn-Jones, R.: NBT-test. Lancet 1973/II, 161–162

  22. Goel, K.M., Vowels, M.R.: Leukocyte function in normal and preterm infants Acta Paediat. Scand. 63, 122–124, 1974

    Google Scholar 

  23. Grush, O.C., Mauer, A.M.: Neutrophil Function and N.B.T. Dye Reduction. Lancet 1969/II, 383

  24. Hellum, K.B., Solberg, C.O.: Positive NBT-Test in acute viral hepatitis. Lancet 1973/I, 1181

  25. Humbert, J.R., Kurtz, M.L., Hathaway, W.E.: Increased Reduction of Nitroblue Tetrazolium by Neutrophils of Newborn Infants. Pediatrics45, 125–128, 1970

    Google Scholar 

  26. Humbert, J.R., Marks, M.I., Hathaway, W.E., Thoren, C.H.: The Histochemical Nitroblue Tetrazolium Reduction Test in the Differential Diagnosis of Acute Infections. Pediatrics48, 259–267 (1971)

    Google Scholar 

  27. Johnston, R.B., Jr.: Screening Test for the Diagnosis of Chronic Granulomatous Disease. Pediatrics43, 122–123, 1969

    Google Scholar 

  28. Kalpaktsoglou, P.K., Padiatellis, C.P., Sofatzis, J.A., Metaxas, C.B.: Evaluation of Nitroblue Tetrazolium test in low birth-weight infants. J. Pediat.84, 441–443, 1974

    Google Scholar 

  29. Kim, S.K., Monz, E., Wehinger, H.: NBT-Test. Ein neues Hilfsmittel für die Diagnostik bakterieller Infektionen. Klin. Pädiat.185, 141–146 (1973)

    Google Scholar 

  30. Magliulo, E., Scevola, D., Bertotto, A., Concia, E., Paga, A.: Differential NBT-Test. Lancet 1974/I, 809–810

  31. Matula, G., Paterson, P.Y.: Spontaneous in vitro Nitro Blue Tetrazolium reduction: a discriminatory test for bacterial infection. J. clin. Invest.49, 62a, 1970

  32. Miescher, P.A.: Problematik und klinische Bedeutung der Leukocyten-Funktionstestung. Klin. Wschr.50, 359–360 (1973)

    Google Scholar 

  33. Nathan, D.G., Baehner, R.L., Weaver, D.K.: Failure of Nitro Blue Tetrazolium Reduction in the Phagocytic Vacuoles of Leukocytes in Chronic Granulomatous Disease. J. Clin. Invest.48, 1895–1904, 1969

    Google Scholar 

  34. Norden, C.W., Reese, R.: Oral Contraceptives and NBT Test. New Engl. J. Med.287, 254 (1972)

    Google Scholar 

  35. Nydegger, U.E., Miescher, A., Anner, R.M., Creighton, D.W., Lambert, P.H., Miescher, P.A.: Serum and Cellular Factor Involvement in Nitroblue Tetrazolium (NBT) Reduction by Human Neutrophils. Klin. Wschr.51, 377–382 (1973)

    Google Scholar 

  36. Ochs, H.D., Igo, R.P.: The NBT slide test: A simple screening method for detecting chronic granulomatous disease and female carriers. J. Pediat.83, 77–82 (1973)

    Google Scholar 

  37. Park, B.H.: The use and limitations of the nitroblue tetrazolium test as a diagnostic aid. J. Pediat.78, 376–378 (1971)

    Google Scholar 

  38. Park, B.H., Fikrig, S.M., Smithwick, E.M.: Infection and nitroblue-tetrazolium reduction by neutrophils. Lancet 1967/I, 532–534

  39. Park, B.H., Good, R.A.: N.B.T. Test Stimulated. Lancet 1970/II, 616

  40. Park, B.H., Holmes, B.M., Rodey, G.E., Good, R.A.: Nitroblue-Tetrazolium Test in Children With Fatal Granulomatous Disease and Newborn Infants. Lancet 1969/I, 157

  41. Pilgrim, U., Gindrat, J.-J., Ruckli, B., Hitzig, W.H.: Nitroblue-Tetrazolium (NBT)-Reduktion in Granulocyten von Kindern mit akuter Leukämie. Schweiz. med. Wschr.104, 147 (1974)

    Google Scholar 

  42. Preisig, E., Hitzig, W.H.: Nitroblue-Tetrazolium Test for the Detection of Chronic Granulomatous Disease — Technical Modification. Europ. J. clin. Invest. 1, 409–412 (1971)

    Google Scholar 

  43. Ramsdale, E.H., Mowbray, J.F.: Positive NBT-Test in pregnancy. Lancet 1973/I, 1245–1247 (1973)

  44. Renner, R.: Untersuchungen über die NADH-Oxydase-Aktivität der neutrophilen Leukocyten. Helvet. paediat. Acta24, 633–643 (1969)

    Google Scholar 

  45. Rubinstein, A., Pelet, B.: False-negative NBT-tests due to transient malfunction of neutrophils. Lancet 1973/I, 382

  46. Seebach, G., Eschenbach, C.: Zur Entstehung cytoplasmatischer Vacuolen von Leukocyten im in-vitro-Versuch. I. Bildung cytoplasmatischer Vacuolen in Abhängigkeit von Entzündungsprozessen. Klin. Wschr.52, 678–687 (1974)

    Google Scholar 

  47. Segal, A.W., Trustey, S.F., Levy, A.J.: Re-Evaluation of Nitroblue-Tetrazolium Test. Lancet 1973/II, 879–883

  48. Shafar, J., Behr, G., Rusius, J.: The nitroblue-tetrazolium test following acute myocardial infarction. J. clin. Path.27, 399–401 (1974)

    Google Scholar 

  49. Shaffer, J.M., Kucera, C.J., Spink, W.W.: The Protection of Intracellular Brucella against Therapeutic Agents and the Bactericidal Action of Serum. J. Exp. Med.97, 77–89 (1953)

    Google Scholar 

  50. Steigbügel, R.T., Johnson, P.K., Remington, J.S.: The nitroblue tetrazolium reduction test versus conventional hematology in the diagnosis of bacterial infection. New Engl. J. Med.290, 235–238 (1974)

    Google Scholar 

  51. Stossel, T.: Evaluation of opsonic and leukocyte function with a spectrometric test in patients with infection and with phagocytic disorders. Blood42, 121–130 (1973)

    Google Scholar 

  52. Thompson, E.N., Chandra, R.K., Cope, W.A., Soothill, J.F.: Leukocyte Abnormality in Both Parents of a Patient with Chronic Granulomatous Disease. Lancet 1969/I, 799–801

  53. Vaucher, A., Wyss, M., Thévoz, F., Knöpfel, M., Miescher, P.A.: La réduction du nitrobleu de tétrazolium par les granulocytes dans divers états cliniques. Schweiz. med. Wschr.100, 2248–2251 (1970)

    Google Scholar 

  54. Wehinger, H., Kim, S.K., Pringsheim, W.: Modified N.B.T. Test in Premature Infants. Lancet 1972/I, 1294

  55. Winchester, J.F., Gordon, A.M., Rowan, A.M., Lindsay, R.M., Black, D.A.: Interpretation of the Nitroblue-Tetrazolium Test in regularly dialysed patients. Lancet 1973/II, 192–293

  56. Windhorst, D.B., Holmes, B., Good, R.: A newly defined x-linked trait in man with demonstration of the lyon effectin carrier females. Lancet 1967/I, 737–739

  57. Wollman, M.R., David, D.S., Brennan, B.L., Lewy, J.E., Stenzel, K.H., Rubin, A.L., Miller, D.R.: The Nitroblue-Tetrazolium Test Usefulness in Detecting Bacterial Infections in Uraemic and Immunosuppressed Renal Transplant Patients. Lancet 1972/I, 289–292

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  1. Univ.-Kinderklinik Marburg a.d. Lahn, Germany

    C. Eschenbach, G. Seebach & St. Müller-Lissner

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Eschenbach, C., Seebach, G. & Müller-Lissner, S. Nitroblau-Tetrazolium-Reduktionskapazität von neutrophilen Granulocyten. Klin Wochenschr 53, 1049–1056 (1975). https://doi.org/10.1007/BF01614380

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